Experiment: KB with Acriflavine 7.75 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0177 and Psyr_0178 are separated by 131 nucleotides Psyr_0178 and Psyr_0179 are separated by 134 nucleotides Psyr_0179 and Psyr_0180 are separated by 2 nucleotides Psyr_0180 and Psyr_0181 are separated by 238 nucleotides Psyr_0181 and Psyr_0182 are separated by 9 nucleotides
Psyr_0177: Psyr_0177 - adenylate cyclase, at 190,574 to 193,420
_0177
Psyr_0178: Psyr_0178 - regulator of nucleoside diphosphate kinase, at 193,552 to 193,959
_0178
Psyr_0179: Psyr_0179 - Protein of unknown function DUF1289, at 194,094 to 194,330
_0179
Psyr_0180: Psyr_0180 - Frataxin-like protein, at 194,333 to 194,665
_0180
Psyr_0181: Psyr_0181 - lipoprotein LppL, at 194,904 to 195,050
_0181
Psyr_0182: Psyr_0182 - diaminopimelate decarboxylase, at 195,060 to 196,307
_0182
Position (kb)
194
195 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 193.136 kb on + strand at 193.136 kb on + strand at 193.136 kb on + strand at 193.136 kb on + strand at 193.136 kb on + strand at 193.143 kb on + strand at 193.144 kb on - strand at 193.144 kb on - strand at 193.144 kb on - strand at 193.193 kb on + strand at 193.193 kb on + strand at 193.194 kb on - strand at 193.226 kb on + strand at 193.226 kb on + strand at 193.226 kb on + strand at 193.242 kb on - strand at 193.301 kb on + strand at 193.301 kb on + strand at 193.301 kb on + strand at 193.301 kb on + strand at 193.301 kb on + strand at 193.302 kb on - strand at 193.302 kb on - strand at 193.302 kb on - strand at 193.302 kb on - strand at 193.302 kb on - strand at 193.554 kb on - strand at 193.583 kb on + strand at 193.583 kb on + strand at 193.583 kb on + strand at 193.583 kb on + strand at 193.745 kb on + strand, within Psyr_0178 at 193.745 kb on + strand, within Psyr_0178 at 193.745 kb on + strand, within Psyr_0178 at 193.746 kb on - strand, within Psyr_0178 at 193.918 kb on + strand, within Psyr_0178 at 193.918 kb on + strand, within Psyr_0178 at 193.918 kb on + strand, within Psyr_0178 at 193.919 kb on - strand at 193.919 kb on - strand at 193.919 kb on - strand at 193.919 kb on - strand at 193.919 kb on - strand at 193.978 kb on + strand at 193.978 kb on + strand at 193.979 kb on - strand at 193.991 kb on + strand at 193.991 kb on + strand at 193.991 kb on + strand at 193.991 kb on + strand at 193.992 kb on - strand at 193.992 kb on - strand at 193.992 kb on - strand at 194.058 kb on + strand at 194.059 kb on - strand at 194.059 kb on - strand at 194.071 kb on + strand at 194.072 kb on - strand at 194.075 kb on + strand at 194.075 kb on + strand at 194.075 kb on + strand at 194.075 kb on + strand at 194.075 kb on + strand at 194.076 kb on - strand at 194.076 kb on - strand at 194.076 kb on - strand at 194.076 kb on - strand at 194.076 kb on - strand at 194.076 kb on - strand at 194.076 kb on - strand at 194.076 kb on - strand at 194.076 kb on - strand at 194.076 kb on - strand at 194.076 kb on - strand at 194.258 kb on + strand, within Psyr_0179 at 194.258 kb on + strand, within Psyr_0179 at 194.259 kb on - strand, within Psyr_0179 at 194.449 kb on - strand, within Psyr_0180 at 194.518 kb on + strand, within Psyr_0180 at 194.518 kb on + strand, within Psyr_0180 at 194.518 kb on + strand, within Psyr_0180 at 194.518 kb on + strand, within Psyr_0180 at 194.519 kb on - strand, within Psyr_0180 at 194.519 kb on - strand, within Psyr_0180 at 194.519 kb on - strand, within Psyr_0180 at 194.553 kb on - strand, within Psyr_0180 at 194.600 kb on + strand, within Psyr_0180 at 194.600 kb on + strand, within Psyr_0180 at 194.601 kb on - strand, within Psyr_0180 at 194.601 kb on - strand, within Psyr_0180 at 194.601 kb on - strand, within Psyr_0180 at 194.612 kb on - strand, within Psyr_0180 at 194.694 kb on + strand at 194.694 kb on + strand at 194.694 kb on + strand at 194.694 kb on + strand at 194.712 kb on + strand at 194.741 kb on + strand at 194.741 kb on + strand at 194.741 kb on + strand at 194.771 kb on - strand at 194.771 kb on - strand at 194.786 kb on + strand at 194.786 kb on + strand at 194.786 kb on + strand at 194.786 kb on + strand at 194.786 kb on + strand at 194.787 kb on - strand at 194.787 kb on - strand at 194.787 kb on - strand at 194.787 kb on - strand at 194.787 kb on - strand at 194.787 kb on - strand at 194.787 kb on - strand at 194.787 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acriflavine 7.75 ug/ml remove 193,136 + -0.5 193,136 + +2.4 193,136 + -0.7 193,136 + -0.9 193,136 + -2.0 193,143 + +0.0 193,144 - +1.1 193,144 - +1.0 193,144 - -0.5 193,193 + -0.6 193,193 + +0.7 193,194 - -0.5 193,226 + +0.8 193,226 + +0.1 193,226 + -0.1 193,242 - +1.1 193,301 + -3.1 193,301 + +1.1 193,301 + -0.8 193,301 + +0.2 193,301 + +0.7 193,302 - -0.7 193,302 - -1.0 193,302 - +0.2 193,302 - +0.8 193,302 - -0.8 193,554 - +0.8 193,583 + -0.1 193,583 + +0.2 193,583 + +1.6 193,583 + -0.7 193,745 + Psyr_0178 0.47 +0.7 193,745 + Psyr_0178 0.47 +0.1 193,745 + Psyr_0178 0.47 -2.0 193,746 - Psyr_0178 0.48 +1.1 193,918 + Psyr_0178 0.90 +1.6 193,918 + Psyr_0178 0.90 +0.3 193,918 + Psyr_0178 0.90 -0.3 193,919 - +0.1 193,919 - -0.9 193,919 - -1.3 193,919 - -0.8 193,919 - +1.0 193,978 + +1.0 193,978 + +0.5 193,979 - -1.0 193,991 + -0.4 193,991 + -0.5 193,991 + +0.7 193,991 + -0.4 193,992 - +0.0 193,992 - +0.3 193,992 - +0.5 194,058 + -0.7 194,059 - +0.1 194,059 - -0.2 194,071 + -0.1 194,072 - -0.0 194,075 + -0.8 194,075 + -0.4 194,075 + +0.7 194,075 + -0.4 194,075 + -1.0 194,076 - -0.4 194,076 - +0.8 194,076 - -1.0 194,076 - -2.4 194,076 - -0.2 194,076 - +0.8 194,076 - +0.8 194,076 - +0.2 194,076 - +0.0 194,076 - -1.2 194,076 - -0.2 194,258 + Psyr_0179 0.69 -1.2 194,258 + Psyr_0179 0.69 -0.4 194,259 - Psyr_0179 0.70 -0.4 194,449 - Psyr_0180 0.35 -0.0 194,518 + Psyr_0180 0.56 +0.6 194,518 + Psyr_0180 0.56 +0.6 194,518 + Psyr_0180 0.56 -0.4 194,518 + Psyr_0180 0.56 +2.1 194,519 - Psyr_0180 0.56 +0.0 194,519 - Psyr_0180 0.56 -0.5 194,519 - Psyr_0180 0.56 +1.3 194,553 - Psyr_0180 0.66 +0.7 194,600 + Psyr_0180 0.80 +1.6 194,600 + Psyr_0180 0.80 +0.3 194,601 - Psyr_0180 0.80 +1.0 194,601 - Psyr_0180 0.80 -0.5 194,601 - Psyr_0180 0.80 +1.3 194,612 - Psyr_0180 0.84 +1.6 194,694 + -0.0 194,694 + -1.5 194,694 + +1.3 194,694 + +0.9 194,712 + +0.3 194,741 + -0.6 194,741 + +0.5 194,741 + -2.3 194,771 - -0.3 194,771 - +1.6 194,786 + +1.6 194,786 + -1.9 194,786 + -1.5 194,786 + -0.0 194,786 + +0.5 194,787 - -0.7 194,787 - -0.7 194,787 - +0.3 194,787 - -1.0 194,787 - -0.3 194,787 - +0.7 194,787 - +1.0 194,787 - +2.0
Or see this region's nucleotide sequence