Experiment: KB with Acriflavine 7.75 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0052 and Psyr_0053 are separated by 46 nucleotides Psyr_0053 and Psyr_0054 overlap by 4 nucleotides Psyr_0054 and Psyr_0055 are separated by 216 nucleotides Psyr_0055 and Psyr_0056 are separated by 77 nucleotides
Psyr_0052: Psyr_0052 - ABC transporter, at 52,475 to 54,385
_0052
Psyr_0053: Psyr_0053 - conserved hypothetical protein, at 54,432 to 54,908
_0053
Psyr_0054: Psyr_0054 - alginate regulatory protein AlgR3, at 54,905 to 55,921
_0054
Psyr_0055: Psyr_0055 - FKBP-type peptidyl-prolyl isomerase, N-terminal:Peptidylprolyl isomerase, FKBP-type, at 56,138 to 56,815
_0055
Psyr_0056: Psyr_0056 - Regulator of RNA polymerase sigma(70) subunit, Rsd/AlgQ, at 56,893 to 57,393
_0056
Position (kb) 54
55
56 Strain fitness (log2 ratio) -2
-1
0
1
2 at 54.018 kb on + strand, within Psyr_0052 at 54.018 kb on + strand, within Psyr_0052 at 54.104 kb on + strand, within Psyr_0052 at 54.138 kb on + strand, within Psyr_0052 at 54.139 kb on - strand, within Psyr_0052 at 54.139 kb on - strand, within Psyr_0052 at 54.190 kb on + strand, within Psyr_0052 at 54.269 kb on - strand at 54.301 kb on + strand at 54.302 kb on - strand at 54.335 kb on + strand at 54.335 kb on + strand at 54.359 kb on + strand at 54.360 kb on - strand at 54.364 kb on + strand at 54.365 kb on - strand at 54.365 kb on - strand at 54.385 kb on + strand at 54.385 kb on + strand at 54.385 kb on + strand at 54.385 kb on + strand at 54.431 kb on + strand at 54.432 kb on - strand at 54.432 kb on - strand at 54.432 kb on - strand at 54.468 kb on + strand at 54.598 kb on + strand, within Psyr_0053 at 54.598 kb on + strand, within Psyr_0053 at 54.598 kb on + strand, within Psyr_0053 at 54.598 kb on + strand, within Psyr_0053 at 54.598 kb on + strand, within Psyr_0053 at 54.599 kb on - strand, within Psyr_0053 at 54.906 kb on + strand at 54.906 kb on + strand at 54.906 kb on + strand at 54.907 kb on - strand at 54.907 kb on - strand at 54.907 kb on - strand at 55.240 kb on + strand, within Psyr_0054 at 55.241 kb on - strand, within Psyr_0054 at 55.438 kb on + strand, within Psyr_0054 at 55.529 kb on - strand, within Psyr_0054 at 55.620 kb on + strand, within Psyr_0054 at 55.620 kb on + strand, within Psyr_0054 at 55.620 kb on + strand, within Psyr_0054 at 55.620 kb on + strand, within Psyr_0054 at 55.620 kb on + strand, within Psyr_0054 at 55.620 kb on + strand, within Psyr_0054 at 55.620 kb on + strand, within Psyr_0054 at 55.620 kb on + strand, within Psyr_0054 at 55.620 kb on + strand, within Psyr_0054 at 55.620 kb on + strand, within Psyr_0054 at 55.620 kb on + strand, within Psyr_0054 at 55.620 kb on + strand, within Psyr_0054 at 55.620 kb on + strand, within Psyr_0054 at 55.621 kb on - strand, within Psyr_0054 at 55.621 kb on - strand, within Psyr_0054 at 55.621 kb on - strand, within Psyr_0054 at 55.621 kb on - strand, within Psyr_0054 at 55.621 kb on - strand, within Psyr_0054 at 55.621 kb on - strand, within Psyr_0054 at 55.621 kb on - strand, within Psyr_0054 at 55.621 kb on - strand, within Psyr_0054 at 55.621 kb on - strand, within Psyr_0054 at 55.621 kb on - strand, within Psyr_0054 at 55.621 kb on - strand, within Psyr_0054 at 55.621 kb on - strand, within Psyr_0054 at 55.621 kb on - strand, within Psyr_0054 at 55.621 kb on - strand, within Psyr_0054 at 55.894 kb on + strand at 55.894 kb on + strand at 55.894 kb on + strand at 55.895 kb on - strand at 55.895 kb on - strand at 55.895 kb on - strand at 55.895 kb on - strand at 55.899 kb on - strand at 55.899 kb on - strand at 55.926 kb on + strand at 55.926 kb on + strand at 55.926 kb on + strand at 55.926 kb on + strand at 55.926 kb on + strand at 55.927 kb on - strand at 55.927 kb on - strand at 55.927 kb on - strand at 55.927 kb on - strand at 55.955 kb on + strand at 55.955 kb on + strand at 55.955 kb on + strand at 55.955 kb on + strand at 55.955 kb on + strand at 55.955 kb on + strand at 55.955 kb on + strand at 55.955 kb on + strand at 55.955 kb on + strand at 55.956 kb on - strand at 55.956 kb on - strand at 55.956 kb on - strand at 55.956 kb on - strand at 55.956 kb on - strand at 55.956 kb on - strand at 55.958 kb on + strand at 55.958 kb on + strand at 55.958 kb on + strand at 55.958 kb on + strand at 55.958 kb on + strand at 55.959 kb on - strand at 55.959 kb on - strand at 56.019 kb on - strand at 56.137 kb on - strand at 56.137 kb on - strand at 56.148 kb on - strand at 56.304 kb on + strand, within Psyr_0055 at 56.305 kb on - strand, within Psyr_0055 at 56.579 kb on + strand, within Psyr_0055 at 56.580 kb on - strand, within Psyr_0055 at 56.580 kb on - strand, within Psyr_0055 at 56.580 kb on - strand, within Psyr_0055 at 56.732 kb on + strand, within Psyr_0055 at 56.732 kb on + strand, within Psyr_0055 at 56.732 kb on + strand, within Psyr_0055 at 56.733 kb on - strand, within Psyr_0055 at 56.771 kb on - strand at 56.782 kb on - strand at 56.782 kb on - strand at 56.813 kb on + strand at 56.813 kb on + strand at 56.813 kb on + strand at 56.814 kb on - strand at 56.814 kb on - strand at 56.814 kb on - strand at 56.856 kb on + strand at 56.856 kb on + strand at 56.856 kb on + strand at 56.856 kb on + strand at 56.857 kb on - strand at 56.857 kb on - strand at 56.857 kb on - strand at 56.857 kb on - strand at 56.873 kb on + strand at 56.873 kb on + strand at 56.873 kb on + strand at 56.873 kb on + strand at 56.874 kb on - strand at 56.874 kb on - strand at 56.874 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acriflavine 7.75 ug/ml remove 54,018 + Psyr_0052 0.81 +0.5 54,018 + Psyr_0052 0.81 +0.2 54,104 + Psyr_0052 0.85 +0.2 54,138 + Psyr_0052 0.87 +0.6 54,139 - Psyr_0052 0.87 +0.4 54,139 - Psyr_0052 0.87 -0.7 54,190 + Psyr_0052 0.90 +0.0 54,269 - +1.1 54,301 + +0.3 54,302 - -0.7 54,335 + +1.7 54,335 + -0.3 54,359 + +0.0 54,360 - -0.5 54,364 + +1.5 54,365 - -0.5 54,365 - +0.3 54,385 + +0.6 54,385 + +0.3 54,385 + -0.3 54,385 + -0.6 54,431 + +0.8 54,432 - +1.3 54,432 - +0.3 54,432 - -2.0 54,468 + +0.4 54,598 + Psyr_0053 0.35 +0.6 54,598 + Psyr_0053 0.35 +0.2 54,598 + Psyr_0053 0.35 -0.6 54,598 + Psyr_0053 0.35 -2.6 54,598 + Psyr_0053 0.35 +0.2 54,599 - Psyr_0053 0.35 +0.5 54,906 + +0.5 54,906 + +0.8 54,906 + +0.5 54,907 - -0.3 54,907 - -0.1 54,907 - -0.8 55,240 + Psyr_0054 0.33 +0.1 55,241 - Psyr_0054 0.33 -0.1 55,438 + Psyr_0054 0.52 +0.8 55,529 - Psyr_0054 0.61 +0.5 55,620 + Psyr_0054 0.70 +1.0 55,620 + Psyr_0054 0.70 +1.2 55,620 + Psyr_0054 0.70 -2.3 55,620 + Psyr_0054 0.70 +0.4 55,620 + Psyr_0054 0.70 +1.0 55,620 + Psyr_0054 0.70 +0.3 55,620 + Psyr_0054 0.70 +0.4 55,620 + Psyr_0054 0.70 -0.5 55,620 + Psyr_0054 0.70 -0.2 55,620 + Psyr_0054 0.70 +1.5 55,620 + Psyr_0054 0.70 +0.7 55,620 + Psyr_0054 0.70 +0.7 55,620 + Psyr_0054 0.70 -0.7 55,621 - Psyr_0054 0.70 +1.0 55,621 - Psyr_0054 0.70 -0.0 55,621 - Psyr_0054 0.70 -0.4 55,621 - Psyr_0054 0.70 -0.5 55,621 - Psyr_0054 0.70 +1.3 55,621 - Psyr_0054 0.70 -0.6 55,621 - Psyr_0054 0.70 +0.5 55,621 - Psyr_0054 0.70 -0.7 55,621 - Psyr_0054 0.70 +1.1 55,621 - Psyr_0054 0.70 +1.0 55,621 - Psyr_0054 0.70 +1.8 55,621 - Psyr_0054 0.70 -1.2 55,621 - Psyr_0054 0.70 +0.8 55,621 - Psyr_0054 0.70 -1.9 55,894 + +0.1 55,894 + +0.6 55,894 + +0.3 55,895 - +1.7 55,895 - -0.2 55,895 - -0.8 55,895 - +0.6 55,899 - -0.5 55,899 - +0.8 55,926 + -0.4 55,926 + -1.0 55,926 + +0.3 55,926 + +0.4 55,926 + -0.0 55,927 - -0.4 55,927 - -0.2 55,927 - +1.4 55,927 - -1.0 55,955 + -0.0 55,955 + -0.8 55,955 + -0.1 55,955 + +1.2 55,955 + -0.5 55,955 + +0.0 55,955 + +1.5 55,955 + -1.3 55,955 + -0.0 55,956 - -0.7 55,956 - +0.1 55,956 - +0.0 55,956 - -1.0 55,956 - -0.9 55,956 - +0.3 55,958 + +0.4 55,958 + +0.5 55,958 + +0.5 55,958 + +0.4 55,958 + -0.1 55,959 - +0.4 55,959 - +1.8 56,019 - +0.5 56,137 - +0.3 56,137 - +0.1 56,148 - -0.3 56,304 + Psyr_0055 0.24 +0.4 56,305 - Psyr_0055 0.25 +0.1 56,579 + Psyr_0055 0.65 -0.4 56,580 - Psyr_0055 0.65 -0.1 56,580 - Psyr_0055 0.65 -1.4 56,580 - Psyr_0055 0.65 +1.0 56,732 + Psyr_0055 0.88 -0.6 56,732 + Psyr_0055 0.88 +0.1 56,732 + Psyr_0055 0.88 +0.2 56,733 - Psyr_0055 0.88 -1.4 56,771 - -0.7 56,782 - -1.2 56,782 - -1.4 56,813 + -0.5 56,813 + -1.5 56,813 + -1.2 56,814 - +0.0 56,814 - +1.4 56,814 - +0.4 56,856 + +0.4 56,856 + -1.1 56,856 + -0.6 56,856 + -0.4 56,857 - -1.1 56,857 - -0.6 56,857 - -0.5 56,857 - +0.4 56,873 + +0.5 56,873 + +0.1 56,873 + +1.4 56,873 + +0.1 56,874 - -0.1 56,874 - +1.5 56,874 - +1.4
Or see this region's nucleotide sequence