Experiment: KB with Acriflavine 7.75 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0016 and Psyr_0017 overlap by 4 nucleotides Psyr_0017 and Psyr_0018 overlap by 4 nucleotides Psyr_0018 and Psyr_0019 are separated by 53 nucleotides
Psyr_0016: Psyr_0016 - TrkA-N:TrkA-C, at 19,329 to 20,702
_0016
Psyr_0017: Psyr_0017 - 16S rRNA m(5)C-967 methyltransferase, at 20,699 to 22,045
_0017
Psyr_0018: Psyr_0018 - methionyl-tRNA formyltransferase, at 22,042 to 22,986
_0018
Psyr_0019: Psyr_0019 - peptide deformylase, at 23,040 to 23,546
_0019
Position (kb) 20
21
22
23 Strain fitness (log2 ratio) -2
-1
0
1
2 at 19.703 kb on - strand, within Psyr_0016 at 19.703 kb on - strand, within Psyr_0016 at 19.703 kb on - strand, within Psyr_0016 at 19.900 kb on + strand, within Psyr_0016 at 19.901 kb on - strand, within Psyr_0016 at 20.003 kb on + strand, within Psyr_0016 at 20.003 kb on + strand, within Psyr_0016 at 20.003 kb on + strand, within Psyr_0016 at 20.011 kb on + strand, within Psyr_0016 at 20.011 kb on + strand, within Psyr_0016 at 20.011 kb on + strand, within Psyr_0016 at 20.012 kb on - strand, within Psyr_0016 at 20.012 kb on - strand, within Psyr_0016 at 20.012 kb on - strand, within Psyr_0016 at 20.012 kb on - strand, within Psyr_0016 at 20.012 kb on - strand, within Psyr_0016 at 20.012 kb on - strand, within Psyr_0016 at 20.012 kb on - strand, within Psyr_0016 at 20.012 kb on - strand, within Psyr_0016 at 20.140 kb on + strand, within Psyr_0016 at 20.140 kb on + strand, within Psyr_0016 at 20.140 kb on + strand, within Psyr_0016 at 20.141 kb on - strand, within Psyr_0016 at 20.141 kb on - strand, within Psyr_0016 at 20.185 kb on - strand, within Psyr_0016 at 20.185 kb on - strand, within Psyr_0016 at 20.185 kb on - strand, within Psyr_0016 at 20.185 kb on - strand, within Psyr_0016 at 20.185 kb on - strand, within Psyr_0016 at 20.221 kb on + strand, within Psyr_0016 at 20.221 kb on + strand, within Psyr_0016 at 20.221 kb on + strand, within Psyr_0016 at 20.225 kb on - strand, within Psyr_0016 at 20.311 kb on + strand, within Psyr_0016 at 20.311 kb on + strand, within Psyr_0016 at 20.311 kb on + strand, within Psyr_0016 at 20.311 kb on + strand, within Psyr_0016 at 20.311 kb on + strand, within Psyr_0016 at 20.311 kb on + strand, within Psyr_0016 at 20.311 kb on + strand, within Psyr_0016 at 20.311 kb on + strand, within Psyr_0016 at 20.311 kb on + strand, within Psyr_0016 at 20.311 kb on + strand, within Psyr_0016 at 20.311 kb on + strand, within Psyr_0016 at 20.311 kb on + strand, within Psyr_0016 at 20.312 kb on - strand, within Psyr_0016 at 20.312 kb on - strand, within Psyr_0016 at 20.312 kb on - strand, within Psyr_0016 at 20.312 kb on - strand, within Psyr_0016 at 20.312 kb on - strand, within Psyr_0016 at 20.312 kb on - strand, within Psyr_0016 at 20.312 kb on - strand, within Psyr_0016 at 20.312 kb on - strand, within Psyr_0016 at 20.312 kb on - strand, within Psyr_0016 at 20.312 kb on - strand, within Psyr_0016 at 20.312 kb on - strand, within Psyr_0016 at 20.343 kb on + strand, within Psyr_0016 at 20.343 kb on + strand, within Psyr_0016 at 20.343 kb on + strand, within Psyr_0016 at 20.343 kb on + strand, within Psyr_0016 at 20.343 kb on + strand, within Psyr_0016 at 20.344 kb on - strand, within Psyr_0016 at 20.367 kb on - strand, within Psyr_0016 at 20.392 kb on + strand, within Psyr_0016 at 20.392 kb on + strand, within Psyr_0016 at 20.441 kb on + strand, within Psyr_0016 at 20.441 kb on + strand, within Psyr_0016 at 20.708 kb on + strand at 20.757 kb on + strand at 20.760 kb on + strand at 20.761 kb on - strand at 20.761 kb on - strand at 20.875 kb on + strand, within Psyr_0017 at 20.938 kb on - strand, within Psyr_0017 at 20.938 kb on - strand, within Psyr_0017 at 20.965 kb on + strand, within Psyr_0017 at 20.966 kb on - strand, within Psyr_0017 at 20.966 kb on - strand, within Psyr_0017 at 20.966 kb on - strand, within Psyr_0017 at 21.254 kb on + strand, within Psyr_0017 at 21.255 kb on - strand, within Psyr_0017 at 21.492 kb on + strand, within Psyr_0017 at 21.493 kb on - strand, within Psyr_0017 at 21.493 kb on - strand, within Psyr_0017 at 21.789 kb on + strand, within Psyr_0017 at 21.789 kb on + strand, within Psyr_0017 at 21.790 kb on - strand, within Psyr_0017 at 22.999 kb on - strand at 22.999 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acriflavine 7.75 ug/ml remove 19,703 - Psyr_0016 0.27 +0.3 19,703 - Psyr_0016 0.27 -0.3 19,703 - Psyr_0016 0.27 -0.3 19,900 + Psyr_0016 0.42 +0.8 19,901 - Psyr_0016 0.42 -0.2 20,003 + Psyr_0016 0.49 -2.0 20,003 + Psyr_0016 0.49 +0.2 20,003 + Psyr_0016 0.49 -0.7 20,011 + Psyr_0016 0.50 -0.5 20,011 + Psyr_0016 0.50 -1.5 20,011 + Psyr_0016 0.50 +0.8 20,012 - Psyr_0016 0.50 -0.0 20,012 - Psyr_0016 0.50 -1.7 20,012 - Psyr_0016 0.50 -1.0 20,012 - Psyr_0016 0.50 +0.1 20,012 - Psyr_0016 0.50 -0.5 20,012 - Psyr_0016 0.50 -0.2 20,012 - Psyr_0016 0.50 -2.3 20,012 - Psyr_0016 0.50 -1.6 20,140 + Psyr_0016 0.59 +0.3 20,140 + Psyr_0016 0.59 -1.7 20,140 + Psyr_0016 0.59 +0.7 20,141 - Psyr_0016 0.59 -0.3 20,141 - Psyr_0016 0.59 +0.1 20,185 - Psyr_0016 0.62 +0.1 20,185 - Psyr_0016 0.62 +0.4 20,185 - Psyr_0016 0.62 -0.4 20,185 - Psyr_0016 0.62 -0.2 20,185 - Psyr_0016 0.62 -0.1 20,221 + Psyr_0016 0.65 -0.0 20,221 + Psyr_0016 0.65 +2.2 20,221 + Psyr_0016 0.65 +0.2 20,225 - Psyr_0016 0.65 -1.0 20,311 + Psyr_0016 0.71 +1.4 20,311 + Psyr_0016 0.71 -0.2 20,311 + Psyr_0016 0.71 +0.8 20,311 + Psyr_0016 0.71 +1.4 20,311 + Psyr_0016 0.71 +0.6 20,311 + Psyr_0016 0.71 -0.0 20,311 + Psyr_0016 0.71 +1.2 20,311 + Psyr_0016 0.71 +0.6 20,311 + Psyr_0016 0.71 -1.6 20,311 + Psyr_0016 0.71 -0.2 20,311 + Psyr_0016 0.71 -0.2 20,311 + Psyr_0016 0.71 +0.5 20,312 - Psyr_0016 0.72 -0.5 20,312 - Psyr_0016 0.72 -0.4 20,312 - Psyr_0016 0.72 -0.4 20,312 - Psyr_0016 0.72 +0.8 20,312 - Psyr_0016 0.72 +0.4 20,312 - Psyr_0016 0.72 +0.1 20,312 - Psyr_0016 0.72 -0.7 20,312 - Psyr_0016 0.72 +0.4 20,312 - Psyr_0016 0.72 -0.2 20,312 - Psyr_0016 0.72 +1.8 20,312 - Psyr_0016 0.72 -2.2 20,343 + Psyr_0016 0.74 +0.5 20,343 + Psyr_0016 0.74 +0.5 20,343 + Psyr_0016 0.74 -0.2 20,343 + Psyr_0016 0.74 +2.2 20,343 + Psyr_0016 0.74 +0.1 20,344 - Psyr_0016 0.74 +1.8 20,367 - Psyr_0016 0.76 -0.2 20,392 + Psyr_0016 0.77 -0.4 20,392 + Psyr_0016 0.77 +0.1 20,441 + Psyr_0016 0.81 +0.0 20,441 + Psyr_0016 0.81 -0.1 20,708 + -1.0 20,757 + -1.7 20,760 + +0.7 20,761 - +0.3 20,761 - +0.4 20,875 + Psyr_0017 0.13 -1.7 20,938 - Psyr_0017 0.18 -0.3 20,938 - Psyr_0017 0.18 -1.2 20,965 + Psyr_0017 0.20 -0.3 20,966 - Psyr_0017 0.20 -1.3 20,966 - Psyr_0017 0.20 -1.6 20,966 - Psyr_0017 0.20 +1.4 21,254 + Psyr_0017 0.41 -0.2 21,255 - Psyr_0017 0.41 -1.3 21,492 + Psyr_0017 0.59 -1.3 21,493 - Psyr_0017 0.59 -1.2 21,493 - Psyr_0017 0.59 -0.7 21,789 + Psyr_0017 0.81 -2.5 21,789 + Psyr_0017 0.81 -0.5 21,790 - Psyr_0017 0.81 +1.0 22,999 - -0.6 22,999 - -1.6
Or see this region's nucleotide sequence