Experiment: R2A with Paraquat dichloride 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS24215 and CA265_RS24220 are separated by 70 nucleotides CA265_RS24220 and CA265_RS24225 are separated by 41 nucleotides CA265_RS24225 and CA265_RS24230 are separated by 129 nucleotides
CA265_RS24215: CA265_RS24215 - hypothetical protein, at 5,898,748 to 5,899,419
_RS24215
CA265_RS24220: CA265_RS24220 - hypothetical protein, at 5,899,490 to 5,899,936
_RS24220
CA265_RS24225: CA265_RS24225 - UDP-N-acetylenolpyruvoylglucosamine reductase, at 5,899,978 to 5,900,994
_RS24225
CA265_RS24230: CA265_RS24230 - RNA polymerase sigma factor, at 5,901,124 to 5,901,654
_RS24230
Position (kb)
5899
5900
5901 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5898.987 kb on + strand, within CA265_RS24215 at 5899.012 kb on - strand, within CA265_RS24215 at 5899.015 kb on - strand, within CA265_RS24215 at 5899.028 kb on + strand, within CA265_RS24215 at 5899.058 kb on - strand, within CA265_RS24215 at 5899.152 kb on + strand, within CA265_RS24215 at 5899.200 kb on + strand, within CA265_RS24215 at 5899.217 kb on + strand, within CA265_RS24215 at 5899.217 kb on + strand, within CA265_RS24215 at 5899.283 kb on - strand, within CA265_RS24215 at 5899.283 kb on - strand, within CA265_RS24215 at 5899.284 kb on + strand, within CA265_RS24215 at 5899.285 kb on - strand, within CA265_RS24215 at 5899.338 kb on - strand, within CA265_RS24215 at 5899.340 kb on - strand, within CA265_RS24215 at 5899.340 kb on - strand, within CA265_RS24215 at 5899.354 kb on - strand at 5899.372 kb on - strand at 5899.427 kb on + strand at 5899.437 kb on + strand at 5899.437 kb on + strand at 5899.485 kb on - strand at 5899.506 kb on - strand at 5899.537 kb on + strand, within CA265_RS24220 at 5899.538 kb on - strand, within CA265_RS24220 at 5899.591 kb on + strand, within CA265_RS24220 at 5899.592 kb on - strand, within CA265_RS24220 at 5899.706 kb on - strand, within CA265_RS24220 at 5899.709 kb on - strand, within CA265_RS24220 at 5899.714 kb on - strand, within CA265_RS24220 at 5899.767 kb on + strand, within CA265_RS24220 at 5899.767 kb on + strand, within CA265_RS24220 at 5899.768 kb on - strand, within CA265_RS24220 at 5899.768 kb on - strand, within CA265_RS24220 at 5899.768 kb on - strand, within CA265_RS24220 at 5899.863 kb on + strand, within CA265_RS24220 at 5899.864 kb on - strand, within CA265_RS24220 at 5899.963 kb on + strand at 5901.065 kb on - strand at 5901.085 kb on + strand at 5901.110 kb on + strand at 5901.111 kb on - strand at 5901.234 kb on + strand, within CA265_RS24230 at 5901.260 kb on - strand, within CA265_RS24230 at 5901.265 kb on - strand, within CA265_RS24230 at 5901.331 kb on - strand, within CA265_RS24230 at 5901.362 kb on + strand, within CA265_RS24230 at 5901.367 kb on - strand, within CA265_RS24230 at 5901.382 kb on - strand, within CA265_RS24230 at 5901.406 kb on - strand, within CA265_RS24230 at 5901.406 kb on - strand, within CA265_RS24230 at 5901.406 kb on - strand, within CA265_RS24230 at 5901.406 kb on - strand, within CA265_RS24230 at 5901.406 kb on - strand, within CA265_RS24230 at 5901.406 kb on - strand, within CA265_RS24230 at 5901.406 kb on - strand, within CA265_RS24230 at 5901.415 kb on - strand, within CA265_RS24230 at 5901.433 kb on - strand, within CA265_RS24230 at 5901.474 kb on - strand, within CA265_RS24230 at 5901.487 kb on + strand, within CA265_RS24230 at 5901.487 kb on + strand, within CA265_RS24230 at 5901.488 kb on - strand, within CA265_RS24230 at 5901.488 kb on - strand, within CA265_RS24230 at 5901.517 kb on + strand, within CA265_RS24230 at 5901.520 kb on - strand, within CA265_RS24230 at 5901.520 kb on - strand, within CA265_RS24230 at 5901.540 kb on + strand, within CA265_RS24230 at 5901.541 kb on - strand, within CA265_RS24230 at 5901.549 kb on + strand, within CA265_RS24230 at 5901.549 kb on + strand, within CA265_RS24230 at 5901.657 kb on + strand at 5901.774 kb on - strand at 5901.828 kb on + strand at 5901.830 kb on + strand at 5901.849 kb on + strand at 5901.865 kb on - strand at 5901.903 kb on - strand at 5901.915 kb on - strand at 5901.915 kb on - strand at 5901.959 kb on + strand at 5901.960 kb on - strand at 5901.960 kb on - strand at 5901.967 kb on + strand at 5901.968 kb on - strand at 5901.972 kb on + strand at 5901.982 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.003 mg/ml remove 5,898,987 + CA265_RS24215 0.36 +0.3 5,899,012 - CA265_RS24215 0.39 +1.2 5,899,015 - CA265_RS24215 0.40 +0.5 5,899,028 + CA265_RS24215 0.42 +0.4 5,899,058 - CA265_RS24215 0.46 -1.7 5,899,152 + CA265_RS24215 0.60 -0.4 5,899,200 + CA265_RS24215 0.67 +1.1 5,899,217 + CA265_RS24215 0.70 +1.6 5,899,217 + CA265_RS24215 0.70 +1.2 5,899,283 - CA265_RS24215 0.80 -1.2 5,899,283 - CA265_RS24215 0.80 +0.3 5,899,284 + CA265_RS24215 0.80 -1.0 5,899,285 - CA265_RS24215 0.80 -1.1 5,899,338 - CA265_RS24215 0.88 +0.5 5,899,340 - CA265_RS24215 0.88 -0.1 5,899,340 - CA265_RS24215 0.88 +0.8 5,899,354 - -1.1 5,899,372 - -0.6 5,899,427 + -0.5 5,899,437 + -0.0 5,899,437 + -0.8 5,899,485 - -1.6 5,899,506 - +0.2 5,899,537 + CA265_RS24220 0.11 +1.0 5,899,538 - CA265_RS24220 0.11 +2.2 5,899,591 + CA265_RS24220 0.23 -0.2 5,899,592 - CA265_RS24220 0.23 +0.4 5,899,706 - CA265_RS24220 0.48 -1.1 5,899,709 - CA265_RS24220 0.49 -0.7 5,899,714 - CA265_RS24220 0.50 -1.2 5,899,767 + CA265_RS24220 0.62 +0.5 5,899,767 + CA265_RS24220 0.62 +0.0 5,899,768 - CA265_RS24220 0.62 -0.4 5,899,768 - CA265_RS24220 0.62 +1.2 5,899,768 - CA265_RS24220 0.62 +0.9 5,899,863 + CA265_RS24220 0.83 -0.1 5,899,864 - CA265_RS24220 0.84 -0.1 5,899,963 + +0.6 5,901,065 - +0.9 5,901,085 + -1.4 5,901,110 + -0.3 5,901,111 - +1.5 5,901,234 + CA265_RS24230 0.21 +1.2 5,901,260 - CA265_RS24230 0.26 +1.1 5,901,265 - CA265_RS24230 0.27 +0.6 5,901,331 - CA265_RS24230 0.39 +0.1 5,901,362 + CA265_RS24230 0.45 +0.8 5,901,367 - CA265_RS24230 0.46 -1.9 5,901,382 - CA265_RS24230 0.49 +0.5 5,901,406 - CA265_RS24230 0.53 +0.4 5,901,406 - CA265_RS24230 0.53 +0.4 5,901,406 - CA265_RS24230 0.53 +0.5 5,901,406 - CA265_RS24230 0.53 +0.5 5,901,406 - CA265_RS24230 0.53 +0.1 5,901,406 - CA265_RS24230 0.53 -0.9 5,901,406 - CA265_RS24230 0.53 +1.9 5,901,415 - CA265_RS24230 0.55 -0.0 5,901,433 - CA265_RS24230 0.58 +0.6 5,901,474 - CA265_RS24230 0.66 +0.3 5,901,487 + CA265_RS24230 0.68 +0.6 5,901,487 + CA265_RS24230 0.68 +0.9 5,901,488 - CA265_RS24230 0.69 -0.6 5,901,488 - CA265_RS24230 0.69 +2.9 5,901,517 + CA265_RS24230 0.74 -1.5 5,901,520 - CA265_RS24230 0.75 +0.5 5,901,520 - CA265_RS24230 0.75 +0.2 5,901,540 + CA265_RS24230 0.78 +0.9 5,901,541 - CA265_RS24230 0.79 +0.4 5,901,549 + CA265_RS24230 0.80 +1.2 5,901,549 + CA265_RS24230 0.80 +1.1 5,901,657 + +3.1 5,901,774 - +0.2 5,901,828 + -0.1 5,901,830 + +1.2 5,901,849 + +0.8 5,901,865 - +0.4 5,901,903 - +0.7 5,901,915 - +1.0 5,901,915 - +0.4 5,901,959 + +1.6 5,901,960 - +1.5 5,901,960 - +0.6 5,901,967 + +1.8 5,901,968 - +1.7 5,901,972 + +1.2 5,901,982 + +0.8
Or see this region's nucleotide sequence