Experiment: R2A with Paraquat dichloride 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS22975 and CA265_RS22980 are separated by 65 nucleotides CA265_RS22980 and CA265_RS22985 are separated by 188 nucleotides CA265_RS22985 and CA265_RS22990 are separated by 280 nucleotides
CA265_RS22975: CA265_RS22975 - 3-keto-alpha-glucoside 1,2-lyase (from data) , at 5,622,528 to 5,623,310
_RS22975
CA265_RS22980: CA265_RS22980 - hypothetical protein, at 5,623,376 to 5,623,693
_RS22980
CA265_RS22985: CA265_RS22985 - helicase SNF2, at 5,623,882 to 5,626,773
_RS22985
CA265_RS22990: CA265_RS22990 - hypothetical protein, at 5,627,054 to 5,628,064
_RS22990
Position (kb)
5623
5624
5625
5626
5627 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 5622.924 kb on + strand, within CA265_RS22975 at 5622.987 kb on - strand, within CA265_RS22975 at 5623.086 kb on - strand, within CA265_RS22975 at 5623.086 kb on - strand, within CA265_RS22975 at 5623.097 kb on + strand, within CA265_RS22975 at 5623.098 kb on - strand, within CA265_RS22975 at 5623.248 kb on - strand at 5623.291 kb on - strand at 5623.293 kb on + strand at 5623.293 kb on + strand at 5623.299 kb on - strand at 5623.300 kb on + strand at 5623.301 kb on - strand at 5623.344 kb on + strand at 5623.415 kb on + strand, within CA265_RS22980 at 5623.466 kb on - strand, within CA265_RS22980 at 5623.496 kb on + strand, within CA265_RS22980 at 5623.576 kb on - strand, within CA265_RS22980 at 5623.576 kb on - strand, within CA265_RS22980 at 5623.576 kb on - strand, within CA265_RS22980 at 5623.664 kb on + strand at 5623.665 kb on - strand at 5623.713 kb on + strand at 5623.717 kb on + strand at 5623.719 kb on - strand at 5623.719 kb on - strand at 5623.719 kb on - strand at 5623.721 kb on - strand at 5623.807 kb on - strand at 5623.827 kb on + strand at 5623.872 kb on + strand at 5623.894 kb on - strand at 5623.902 kb on + strand at 5623.903 kb on - strand at 5623.949 kb on - strand at 5623.990 kb on + strand at 5623.991 kb on - strand at 5624.067 kb on + strand at 5624.250 kb on - strand, within CA265_RS22985 at 5624.331 kb on + strand, within CA265_RS22985 at 5624.332 kb on - strand, within CA265_RS22985 at 5624.416 kb on + strand, within CA265_RS22985 at 5624.543 kb on + strand, within CA265_RS22985 at 5624.546 kb on + strand, within CA265_RS22985 at 5624.602 kb on + strand, within CA265_RS22985 at 5624.662 kb on + strand, within CA265_RS22985 at 5624.662 kb on + strand, within CA265_RS22985 at 5624.712 kb on - strand, within CA265_RS22985 at 5624.712 kb on - strand, within CA265_RS22985 at 5624.736 kb on + strand, within CA265_RS22985 at 5624.756 kb on - strand, within CA265_RS22985 at 5624.824 kb on - strand, within CA265_RS22985 at 5624.824 kb on - strand, within CA265_RS22985 at 5624.878 kb on - strand, within CA265_RS22985 at 5624.901 kb on + strand, within CA265_RS22985 at 5624.901 kb on + strand, within CA265_RS22985 at 5624.936 kb on - strand, within CA265_RS22985 at 5624.957 kb on + strand, within CA265_RS22985 at 5624.958 kb on - strand, within CA265_RS22985 at 5624.965 kb on - strand, within CA265_RS22985 at 5624.971 kb on - strand, within CA265_RS22985 at 5624.971 kb on - strand, within CA265_RS22985 at 5624.979 kb on - strand, within CA265_RS22985 at 5625.004 kb on - strand, within CA265_RS22985 at 5625.013 kb on - strand, within CA265_RS22985 at 5625.022 kb on - strand, within CA265_RS22985 at 5625.031 kb on + strand, within CA265_RS22985 at 5625.057 kb on + strand, within CA265_RS22985 at 5625.078 kb on - strand, within CA265_RS22985 at 5625.078 kb on - strand, within CA265_RS22985 at 5625.103 kb on - strand, within CA265_RS22985 at 5625.164 kb on - strand, within CA265_RS22985 at 5625.221 kb on + strand, within CA265_RS22985 at 5625.246 kb on + strand, within CA265_RS22985 at 5625.247 kb on - strand, within CA265_RS22985 at 5625.249 kb on - strand, within CA265_RS22985 at 5625.272 kb on - strand, within CA265_RS22985 at 5625.360 kb on + strand, within CA265_RS22985 at 5625.391 kb on + strand, within CA265_RS22985 at 5625.458 kb on + strand, within CA265_RS22985 at 5625.465 kb on + strand, within CA265_RS22985 at 5625.483 kb on + strand, within CA265_RS22985 at 5625.534 kb on + strand, within CA265_RS22985 at 5625.535 kb on - strand, within CA265_RS22985 at 5625.711 kb on + strand, within CA265_RS22985 at 5625.730 kb on - strand, within CA265_RS22985 at 5625.741 kb on - strand, within CA265_RS22985 at 5625.901 kb on + strand, within CA265_RS22985 at 5625.902 kb on - strand, within CA265_RS22985 at 5625.902 kb on - strand, within CA265_RS22985 at 5625.902 kb on - strand, within CA265_RS22985 at 5625.937 kb on - strand, within CA265_RS22985 at 5625.952 kb on - strand, within CA265_RS22985 at 5626.052 kb on + strand, within CA265_RS22985 at 5626.053 kb on - strand, within CA265_RS22985 at 5626.053 kb on - strand, within CA265_RS22985 at 5626.098 kb on - strand, within CA265_RS22985 at 5626.101 kb on + strand, within CA265_RS22985 at 5626.129 kb on - strand, within CA265_RS22985 at 5626.137 kb on + strand, within CA265_RS22985 at 5626.170 kb on + strand, within CA265_RS22985 at 5626.192 kb on + strand, within CA265_RS22985 at 5626.192 kb on + strand, within CA265_RS22985 at 5626.193 kb on - strand, within CA265_RS22985 at 5626.264 kb on + strand, within CA265_RS22985 at 5626.394 kb on - strand, within CA265_RS22985 at 5626.397 kb on + strand, within CA265_RS22985 at 5626.397 kb on + strand, within CA265_RS22985 at 5626.532 kb on + strand at 5626.587 kb on - strand at 5626.594 kb on - strand at 5626.600 kb on + strand at 5626.712 kb on + strand at 5626.734 kb on - strand at 5626.744 kb on - strand at 5626.753 kb on - strand at 5626.758 kb on + strand at 5626.769 kb on - strand at 5626.800 kb on + strand at 5626.801 kb on - strand at 5626.824 kb on + strand at 5626.829 kb on + strand at 5626.840 kb on + strand at 5626.840 kb on + strand at 5626.909 kb on + strand at 5626.972 kb on - strand at 5626.973 kb on + strand at 5626.988 kb on + strand at 5627.005 kb on + strand at 5627.012 kb on - strand at 5627.052 kb on - strand at 5627.135 kb on + strand at 5627.138 kb on - strand at 5627.209 kb on - strand, within CA265_RS22990 at 5627.253 kb on - strand, within CA265_RS22990 at 5627.263 kb on - strand, within CA265_RS22990 at 5627.350 kb on - strand, within CA265_RS22990 at 5627.357 kb on + strand, within CA265_RS22990 at 5627.357 kb on + strand, within CA265_RS22990 at 5627.359 kb on + strand, within CA265_RS22990 at 5627.437 kb on - strand, within CA265_RS22990 at 5627.447 kb on + strand, within CA265_RS22990 at 5627.447 kb on + strand, within CA265_RS22990 at 5627.447 kb on + strand, within CA265_RS22990 at 5627.448 kb on - strand, within CA265_RS22990 at 5627.448 kb on - strand, within CA265_RS22990 at 5627.518 kb on + strand, within CA265_RS22990 at 5627.542 kb on - strand, within CA265_RS22990 at 5627.615 kb on - strand, within CA265_RS22990 at 5627.615 kb on - strand, within CA265_RS22990 at 5627.623 kb on - strand, within CA265_RS22990 at 5627.624 kb on + strand, within CA265_RS22990 at 5627.625 kb on - strand, within CA265_RS22990 at 5627.625 kb on - strand, within CA265_RS22990 at 5627.626 kb on + strand, within CA265_RS22990 at 5627.626 kb on + strand, within CA265_RS22990 at 5627.750 kb on - strand, within CA265_RS22990 at 5627.750 kb on - strand, within CA265_RS22990
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.003 mg/ml remove 5,622,924 + CA265_RS22975 0.51 +0.2 5,622,987 - CA265_RS22975 0.59 +1.3 5,623,086 - CA265_RS22975 0.71 +0.8 5,623,086 - CA265_RS22975 0.71 -0.1 5,623,097 + CA265_RS22975 0.73 -1.1 5,623,098 - CA265_RS22975 0.73 +0.2 5,623,248 - -0.3 5,623,291 - -0.7 5,623,293 + -0.8 5,623,293 + -0.6 5,623,299 - +1.0 5,623,300 + +0.1 5,623,301 - -0.5 5,623,344 + -0.9 5,623,415 + CA265_RS22980 0.12 +0.5 5,623,466 - CA265_RS22980 0.28 +1.9 5,623,496 + CA265_RS22980 0.38 -0.4 5,623,576 - CA265_RS22980 0.63 -0.1 5,623,576 - CA265_RS22980 0.63 -1.0 5,623,576 - CA265_RS22980 0.63 +1.6 5,623,664 + -1.4 5,623,665 - -0.4 5,623,713 + -2.2 5,623,717 + -0.7 5,623,719 - -0.8 5,623,719 - -0.7 5,623,719 - +0.5 5,623,721 - -0.5 5,623,807 - -0.5 5,623,827 + +0.2 5,623,872 + -3.4 5,623,894 - -3.5 5,623,902 + -1.9 5,623,903 - -3.3 5,623,949 - -1.5 5,623,990 + -0.1 5,623,991 - -0.1 5,624,067 + -2.3 5,624,250 - CA265_RS22985 0.13 -0.5 5,624,331 + CA265_RS22985 0.16 -2.4 5,624,332 - CA265_RS22985 0.16 -1.1 5,624,416 + CA265_RS22985 0.18 +3.1 5,624,543 + CA265_RS22985 0.23 -4.3 5,624,546 + CA265_RS22985 0.23 -2.1 5,624,602 + CA265_RS22985 0.25 -0.9 5,624,662 + CA265_RS22985 0.27 -0.4 5,624,662 + CA265_RS22985 0.27 -2.5 5,624,712 - CA265_RS22985 0.29 -2.8 5,624,712 - CA265_RS22985 0.29 -0.2 5,624,736 + CA265_RS22985 0.30 -1.9 5,624,756 - CA265_RS22985 0.30 +0.2 5,624,824 - CA265_RS22985 0.33 -3.3 5,624,824 - CA265_RS22985 0.33 -2.3 5,624,878 - CA265_RS22985 0.34 +0.7 5,624,901 + CA265_RS22985 0.35 -1.1 5,624,901 + CA265_RS22985 0.35 -2.4 5,624,936 - CA265_RS22985 0.36 -1.4 5,624,957 + CA265_RS22985 0.37 -2.9 5,624,958 - CA265_RS22985 0.37 -2.0 5,624,965 - CA265_RS22985 0.37 -1.5 5,624,971 - CA265_RS22985 0.38 -0.8 5,624,971 - CA265_RS22985 0.38 -2.1 5,624,979 - CA265_RS22985 0.38 -1.6 5,625,004 - CA265_RS22985 0.39 -1.6 5,625,013 - CA265_RS22985 0.39 -1.4 5,625,022 - CA265_RS22985 0.39 -0.7 5,625,031 + CA265_RS22985 0.40 -0.5 5,625,057 + CA265_RS22985 0.41 -0.5 5,625,078 - CA265_RS22985 0.41 -0.4 5,625,078 - CA265_RS22985 0.41 -0.9 5,625,103 - CA265_RS22985 0.42 -2.1 5,625,164 - CA265_RS22985 0.44 -2.0 5,625,221 + CA265_RS22985 0.46 -1.6 5,625,246 + CA265_RS22985 0.47 -1.1 5,625,247 - CA265_RS22985 0.47 -0.5 5,625,249 - CA265_RS22985 0.47 -2.8 5,625,272 - CA265_RS22985 0.48 -2.4 5,625,360 + CA265_RS22985 0.51 -0.3 5,625,391 + CA265_RS22985 0.52 -2.3 5,625,458 + CA265_RS22985 0.54 -1.8 5,625,465 + CA265_RS22985 0.55 -2.2 5,625,483 + CA265_RS22985 0.55 -2.3 5,625,534 + CA265_RS22985 0.57 +0.2 5,625,535 - CA265_RS22985 0.57 +0.1 5,625,711 + CA265_RS22985 0.63 -1.6 5,625,730 - CA265_RS22985 0.64 -1.2 5,625,741 - CA265_RS22985 0.64 -2.1 5,625,901 + CA265_RS22985 0.70 -1.2 5,625,902 - CA265_RS22985 0.70 -2.0 5,625,902 - CA265_RS22985 0.70 -2.3 5,625,902 - CA265_RS22985 0.70 -2.7 5,625,937 - CA265_RS22985 0.71 -1.0 5,625,952 - CA265_RS22985 0.72 -1.0 5,626,052 + CA265_RS22985 0.75 -0.8 5,626,053 - CA265_RS22985 0.75 -2.2 5,626,053 - CA265_RS22985 0.75 -1.0 5,626,098 - CA265_RS22985 0.77 -1.2 5,626,101 + CA265_RS22985 0.77 -1.0 5,626,129 - CA265_RS22985 0.78 -2.4 5,626,137 + CA265_RS22985 0.78 -0.8 5,626,170 + CA265_RS22985 0.79 -0.7 5,626,192 + CA265_RS22985 0.80 -1.5 5,626,192 + CA265_RS22985 0.80 -1.9 5,626,193 - CA265_RS22985 0.80 +0.3 5,626,264 + CA265_RS22985 0.82 -0.1 5,626,394 - CA265_RS22985 0.87 -1.8 5,626,397 + CA265_RS22985 0.87 -1.2 5,626,397 + CA265_RS22985 0.87 +0.3 5,626,532 + -0.5 5,626,587 - -1.8 5,626,594 - +0.3 5,626,600 + -0.6 5,626,712 + -1.9 5,626,734 - +0.6 5,626,744 - -0.7 5,626,753 - -1.8 5,626,758 + -1.7 5,626,769 - -1.1 5,626,800 + -2.4 5,626,801 - +0.7 5,626,824 + -1.5 5,626,829 + +0.0 5,626,840 + +0.3 5,626,840 + -1.4 5,626,909 + -0.3 5,626,972 - -1.2 5,626,973 + -1.1 5,626,988 + -0.4 5,627,005 + -0.8 5,627,012 - -0.0 5,627,052 - -0.9 5,627,135 + +0.0 5,627,138 - -1.7 5,627,209 - CA265_RS22990 0.15 +0.1 5,627,253 - CA265_RS22990 0.20 -0.3 5,627,263 - CA265_RS22990 0.21 +0.1 5,627,350 - CA265_RS22990 0.29 -0.4 5,627,357 + CA265_RS22990 0.30 +0.7 5,627,357 + CA265_RS22990 0.30 -0.9 5,627,359 + CA265_RS22990 0.30 +2.2 5,627,437 - CA265_RS22990 0.38 -1.3 5,627,447 + CA265_RS22990 0.39 -1.1 5,627,447 + CA265_RS22990 0.39 -0.3 5,627,447 + CA265_RS22990 0.39 -1.0 5,627,448 - CA265_RS22990 0.39 -0.5 5,627,448 - CA265_RS22990 0.39 +0.0 5,627,518 + CA265_RS22990 0.46 +1.3 5,627,542 - CA265_RS22990 0.48 -0.1 5,627,615 - CA265_RS22990 0.55 +0.7 5,627,615 - CA265_RS22990 0.55 -0.8 5,627,623 - CA265_RS22990 0.56 -1.1 5,627,624 + CA265_RS22990 0.56 -0.8 5,627,625 - CA265_RS22990 0.56 +0.1 5,627,625 - CA265_RS22990 0.56 -0.2 5,627,626 + CA265_RS22990 0.57 -0.2 5,627,626 + CA265_RS22990 0.57 -0.2 5,627,750 - CA265_RS22990 0.69 +1.2 5,627,750 - CA265_RS22990 0.69 +0.8
Or see this region's nucleotide sequence