Experiment: R2A with Paraquat dichloride 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS16520 and CA265_RS16525 are separated by 490 nucleotides CA265_RS16525 and CA265_RS16530 are separated by 0 nucleotides CA265_RS16530 and CA265_RS16535 overlap by 4 nucleotides
CA265_RS16520: CA265_RS16520 - sterol desaturase, at 4,155,870 to 4,157,009
_RS16520
CA265_RS16525: CA265_RS16525 - FAD-dependent oxidoreductase, at 4,157,500 to 4,158,630
_RS16525
CA265_RS16530: CA265_RS16530 - methyltransferase, at 4,158,631 to 4,159,344
_RS16530
CA265_RS16535: CA265_RS16535 - type III polyketide synthase, at 4,159,341 to 4,160,393
_RS16535
Position (kb)
4157
4158
4159 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4156.550 kb on - strand, within CA265_RS16520 at 4156.680 kb on + strand, within CA265_RS16520 at 4156.681 kb on - strand, within CA265_RS16520 at 4156.681 kb on - strand, within CA265_RS16520 at 4156.691 kb on + strand, within CA265_RS16520 at 4156.806 kb on + strand, within CA265_RS16520 at 4156.807 kb on - strand, within CA265_RS16520 at 4156.847 kb on + strand, within CA265_RS16520 at 4156.860 kb on - strand, within CA265_RS16520 at 4156.860 kb on - strand, within CA265_RS16520 at 4156.864 kb on - strand, within CA265_RS16520 at 4156.903 kb on + strand at 4156.947 kb on + strand at 4157.007 kb on + strand at 4157.008 kb on - strand at 4157.008 kb on - strand at 4157.042 kb on - strand at 4157.048 kb on - strand at 4157.048 kb on - strand at 4157.051 kb on + strand at 4157.051 kb on + strand at 4157.052 kb on - strand at 4157.055 kb on + strand at 4157.082 kb on - strand at 4157.084 kb on + strand at 4157.085 kb on - strand at 4157.086 kb on + strand at 4157.152 kb on - strand at 4157.366 kb on - strand at 4157.371 kb on + strand at 4157.372 kb on - strand at 4157.372 kb on - strand at 4157.373 kb on + strand at 4157.374 kb on - strand at 4157.499 kb on + strand at 4157.499 kb on + strand at 4157.583 kb on - strand at 4157.583 kb on - strand at 4157.607 kb on + strand at 4157.607 kb on + strand at 4157.607 kb on + strand at 4157.638 kb on + strand, within CA265_RS16525 at 4157.748 kb on + strand, within CA265_RS16525 at 4157.784 kb on - strand, within CA265_RS16525 at 4157.791 kb on + strand, within CA265_RS16525 at 4157.791 kb on + strand, within CA265_RS16525 at 4157.793 kb on + strand, within CA265_RS16525 at 4157.837 kb on + strand, within CA265_RS16525 at 4157.883 kb on + strand, within CA265_RS16525 at 4157.926 kb on + strand, within CA265_RS16525 at 4158.004 kb on + strand, within CA265_RS16525 at 4158.025 kb on + strand, within CA265_RS16525 at 4158.026 kb on - strand, within CA265_RS16525 at 4158.026 kb on - strand, within CA265_RS16525 at 4158.236 kb on + strand, within CA265_RS16525 at 4158.290 kb on + strand, within CA265_RS16525 at 4158.293 kb on - strand, within CA265_RS16525 at 4158.293 kb on - strand, within CA265_RS16525 at 4158.295 kb on - strand, within CA265_RS16525 at 4158.301 kb on - strand, within CA265_RS16525 at 4158.451 kb on + strand, within CA265_RS16525 at 4158.459 kb on - strand, within CA265_RS16525 at 4158.504 kb on - strand, within CA265_RS16525 at 4158.582 kb on + strand at 4158.628 kb on + strand at 4158.643 kb on - strand at 4158.649 kb on + strand at 4158.652 kb on - strand at 4158.652 kb on - strand at 4158.652 kb on - strand at 4158.673 kb on - strand at 4158.702 kb on - strand at 4158.757 kb on - strand, within CA265_RS16530 at 4158.762 kb on + strand, within CA265_RS16530 at 4158.814 kb on - strand, within CA265_RS16530 at 4158.814 kb on - strand, within CA265_RS16530 at 4158.815 kb on + strand, within CA265_RS16530 at 4158.849 kb on + strand, within CA265_RS16530 at 4158.849 kb on + strand, within CA265_RS16530 at 4158.849 kb on + strand, within CA265_RS16530 at 4158.849 kb on + strand, within CA265_RS16530 at 4158.850 kb on - strand, within CA265_RS16530 at 4158.876 kb on + strand, within CA265_RS16530 at 4158.890 kb on + strand, within CA265_RS16530 at 4158.891 kb on - strand, within CA265_RS16530 at 4158.891 kb on - strand, within CA265_RS16530 at 4158.891 kb on - strand, within CA265_RS16530 at 4158.911 kb on + strand, within CA265_RS16530 at 4158.977 kb on - strand, within CA265_RS16530 at 4158.977 kb on - strand, within CA265_RS16530 at 4158.990 kb on + strand, within CA265_RS16530 at 4158.992 kb on + strand, within CA265_RS16530 at 4159.004 kb on + strand, within CA265_RS16530 at 4159.029 kb on + strand, within CA265_RS16530 at 4159.038 kb on + strand, within CA265_RS16530 at 4159.039 kb on - strand, within CA265_RS16530 at 4159.050 kb on - strand, within CA265_RS16530 at 4159.074 kb on + strand, within CA265_RS16530 at 4159.112 kb on + strand, within CA265_RS16530 at 4159.112 kb on + strand, within CA265_RS16530 at 4159.153 kb on + strand, within CA265_RS16530 at 4159.154 kb on - strand, within CA265_RS16530 at 4159.157 kb on - strand, within CA265_RS16530 at 4159.164 kb on + strand, within CA265_RS16530 at 4159.177 kb on + strand, within CA265_RS16530 at 4159.197 kb on + strand, within CA265_RS16530 at 4159.218 kb on - strand, within CA265_RS16530 at 4159.218 kb on - strand, within CA265_RS16530 at 4159.274 kb on + strand at 4159.341 kb on - strand at 4159.354 kb on + strand at 4159.354 kb on + strand at 4159.363 kb on + strand at 4159.408 kb on + strand at 4159.564 kb on - strand, within CA265_RS16535 at 4159.568 kb on - strand, within CA265_RS16535 at 4159.579 kb on + strand, within CA265_RS16535 at 4159.593 kb on - strand, within CA265_RS16535
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.003 mg/ml remove 4,156,550 - CA265_RS16520 0.60 -2.1 4,156,680 + CA265_RS16520 0.71 -1.1 4,156,681 - CA265_RS16520 0.71 -1.7 4,156,681 - CA265_RS16520 0.71 +2.0 4,156,691 + CA265_RS16520 0.72 +0.4 4,156,806 + CA265_RS16520 0.82 +0.2 4,156,807 - CA265_RS16520 0.82 +0.7 4,156,847 + CA265_RS16520 0.86 -1.0 4,156,860 - CA265_RS16520 0.87 -0.6 4,156,860 - CA265_RS16520 0.87 +0.3 4,156,864 - CA265_RS16520 0.87 -0.9 4,156,903 + +0.3 4,156,947 + +0.3 4,157,007 + +0.7 4,157,008 - -1.8 4,157,008 - -0.6 4,157,042 - +0.1 4,157,048 - -0.3 4,157,048 - -0.3 4,157,051 + +0.2 4,157,051 + -1.0 4,157,052 - -0.4 4,157,055 + -0.3 4,157,082 - +0.4 4,157,084 + +0.5 4,157,085 - -0.9 4,157,086 + +0.2 4,157,152 - -0.3 4,157,366 - -2.5 4,157,371 + -1.3 4,157,372 - +0.1 4,157,372 - -0.6 4,157,373 + +1.5 4,157,374 - -1.4 4,157,499 + +0.9 4,157,499 + +1.0 4,157,583 - -1.0 4,157,583 - +0.6 4,157,607 + +0.9 4,157,607 + +0.3 4,157,607 + +1.2 4,157,638 + CA265_RS16525 0.12 +0.8 4,157,748 + CA265_RS16525 0.22 +1.4 4,157,784 - CA265_RS16525 0.25 -0.1 4,157,791 + CA265_RS16525 0.26 +0.9 4,157,791 + CA265_RS16525 0.26 +2.3 4,157,793 + CA265_RS16525 0.26 +0.8 4,157,837 + CA265_RS16525 0.30 +1.1 4,157,883 + CA265_RS16525 0.34 +0.2 4,157,926 + CA265_RS16525 0.38 -1.3 4,158,004 + CA265_RS16525 0.45 +1.3 4,158,025 + CA265_RS16525 0.46 -1.2 4,158,026 - CA265_RS16525 0.47 +0.0 4,158,026 - CA265_RS16525 0.47 -0.3 4,158,236 + CA265_RS16525 0.65 +2.8 4,158,290 + CA265_RS16525 0.70 +2.5 4,158,293 - CA265_RS16525 0.70 +0.5 4,158,293 - CA265_RS16525 0.70 -0.0 4,158,295 - CA265_RS16525 0.70 -0.8 4,158,301 - CA265_RS16525 0.71 +0.2 4,158,451 + CA265_RS16525 0.84 +2.4 4,158,459 - CA265_RS16525 0.85 -0.4 4,158,504 - CA265_RS16525 0.89 +0.1 4,158,582 + +1.9 4,158,628 + +0.6 4,158,643 - +1.5 4,158,649 + +0.2 4,158,652 - -0.2 4,158,652 - -0.2 4,158,652 - -0.1 4,158,673 - -0.9 4,158,702 - -0.7 4,158,757 - CA265_RS16530 0.18 -0.6 4,158,762 + CA265_RS16530 0.18 +1.6 4,158,814 - CA265_RS16530 0.26 -1.2 4,158,814 - CA265_RS16530 0.26 +0.1 4,158,815 + CA265_RS16530 0.26 +0.7 4,158,849 + CA265_RS16530 0.31 -0.2 4,158,849 + CA265_RS16530 0.31 +1.2 4,158,849 + CA265_RS16530 0.31 -0.4 4,158,849 + CA265_RS16530 0.31 -1.9 4,158,850 - CA265_RS16530 0.31 -0.6 4,158,876 + CA265_RS16530 0.34 -0.6 4,158,890 + CA265_RS16530 0.36 -0.0 4,158,891 - CA265_RS16530 0.36 +0.5 4,158,891 - CA265_RS16530 0.36 -0.8 4,158,891 - CA265_RS16530 0.36 -1.2 4,158,911 + CA265_RS16530 0.39 +0.5 4,158,977 - CA265_RS16530 0.48 -0.9 4,158,977 - CA265_RS16530 0.48 +0.6 4,158,990 + CA265_RS16530 0.50 +0.6 4,158,992 + CA265_RS16530 0.51 +0.7 4,159,004 + CA265_RS16530 0.52 +0.5 4,159,029 + CA265_RS16530 0.56 -0.0 4,159,038 + CA265_RS16530 0.57 +0.9 4,159,039 - CA265_RS16530 0.57 -0.2 4,159,050 - CA265_RS16530 0.59 -0.7 4,159,074 + CA265_RS16530 0.62 +1.0 4,159,112 + CA265_RS16530 0.67 +0.6 4,159,112 + CA265_RS16530 0.67 +1.3 4,159,153 + CA265_RS16530 0.73 -1.3 4,159,154 - CA265_RS16530 0.73 -0.6 4,159,157 - CA265_RS16530 0.74 -2.1 4,159,164 + CA265_RS16530 0.75 +0.7 4,159,177 + CA265_RS16530 0.76 +0.2 4,159,197 + CA265_RS16530 0.79 -0.3 4,159,218 - CA265_RS16530 0.82 -0.4 4,159,218 - CA265_RS16530 0.82 -0.6 4,159,274 + +0.5 4,159,341 - -1.3 4,159,354 + +1.7 4,159,354 + -1.2 4,159,363 + +0.7 4,159,408 + +0.7 4,159,564 - CA265_RS16535 0.21 +1.2 4,159,568 - CA265_RS16535 0.22 -0.2 4,159,579 + CA265_RS16535 0.23 +1.8 4,159,593 - CA265_RS16535 0.24 +0.3
Or see this region's nucleotide sequence