Experiment: R2A with Paraquat dichloride 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS08815 and CA265_RS08820 are separated by 53 nucleotides CA265_RS08820 and CA265_RS08825 overlap by 1 nucleotides CA265_RS08825 and CA265_RS08830 are separated by 119 nucleotides CA265_RS08830 and CA265_RS08835 are separated by 152 nucleotides
CA265_RS08815: CA265_RS08815 - hypothetical protein, at 2,206,541 to 2,206,903
_RS08815
CA265_RS08820: CA265_RS08820 - hypothetical protein, at 2,206,957 to 2,207,739
_RS08820
CA265_RS08825: CA265_RS08825 - tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA, at 2,207,739 to 2,208,668
_RS08825
CA265_RS08830: CA265_RS08830 - hypothetical protein, at 2,208,788 to 2,209,312
_RS08830
CA265_RS08835: CA265_RS08835 - hypothetical protein, at 2,209,465 to 2,210,016
_RS08835
Position (kb)
2207
2208
2209 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2206.748 kb on - strand, within CA265_RS08815 at 2206.775 kb on - strand, within CA265_RS08815 at 2206.780 kb on - strand, within CA265_RS08815 at 2206.857 kb on + strand, within CA265_RS08815 at 2206.874 kb on - strand at 2206.974 kb on + strand at 2207.001 kb on + strand at 2207.001 kb on + strand at 2207.002 kb on - strand at 2207.063 kb on + strand, within CA265_RS08820 at 2207.064 kb on - strand, within CA265_RS08820 at 2207.081 kb on + strand, within CA265_RS08820 at 2207.081 kb on + strand, within CA265_RS08820 at 2207.097 kb on - strand, within CA265_RS08820 at 2207.167 kb on - strand, within CA265_RS08820 at 2207.173 kb on - strand, within CA265_RS08820 at 2207.279 kb on + strand, within CA265_RS08820 at 2207.498 kb on + strand, within CA265_RS08820 at 2207.532 kb on + strand, within CA265_RS08820 at 2207.554 kb on + strand, within CA265_RS08820 at 2207.587 kb on + strand, within CA265_RS08820 at 2207.588 kb on - strand, within CA265_RS08820 at 2207.588 kb on - strand, within CA265_RS08820 at 2207.604 kb on + strand, within CA265_RS08820 at 2207.834 kb on + strand, within CA265_RS08825 at 2207.947 kb on + strand, within CA265_RS08825 at 2208.011 kb on + strand, within CA265_RS08825 at 2208.012 kb on - strand at 2208.060 kb on + strand, within CA265_RS08825 at 2208.060 kb on + strand, within CA265_RS08825 at 2208.061 kb on - strand, within CA265_RS08825 at 2208.069 kb on - strand at 2208.119 kb on - strand, within CA265_RS08825 at 2208.136 kb on + strand, within CA265_RS08825 at 2208.137 kb on - strand, within CA265_RS08825 at 2208.249 kb on + strand, within CA265_RS08825 at 2208.260 kb on + strand, within CA265_RS08825 at 2208.260 kb on + strand, within CA265_RS08825 at 2208.260 kb on + strand, within CA265_RS08825 at 2208.261 kb on - strand, within CA265_RS08825 at 2208.263 kb on + strand, within CA265_RS08825 at 2208.264 kb on - strand, within CA265_RS08825 at 2208.294 kb on - strand, within CA265_RS08825 at 2208.371 kb on + strand, within CA265_RS08825 at 2208.382 kb on + strand, within CA265_RS08825 at 2208.416 kb on + strand, within CA265_RS08825 at 2208.416 kb on + strand, within CA265_RS08825 at 2208.417 kb on - strand, within CA265_RS08825 at 2208.417 kb on - strand, within CA265_RS08825 at 2208.508 kb on + strand, within CA265_RS08825 at 2208.551 kb on + strand, within CA265_RS08825 at 2208.673 kb on + strand at 2208.758 kb on + strand at 2208.758 kb on + strand at 2208.814 kb on + strand at 2208.815 kb on - strand at 2208.848 kb on - strand, within CA265_RS08830 at 2208.892 kb on + strand, within CA265_RS08830 at 2208.892 kb on + strand, within CA265_RS08830 at 2208.893 kb on - strand, within CA265_RS08830 at 2208.902 kb on - strand, within CA265_RS08830 at 2208.904 kb on - strand, within CA265_RS08830 at 2208.907 kb on + strand, within CA265_RS08830 at 2208.910 kb on + strand, within CA265_RS08830 at 2208.910 kb on + strand, within CA265_RS08830 at 2208.920 kb on + strand, within CA265_RS08830 at 2208.927 kb on + strand, within CA265_RS08830 at 2208.927 kb on + strand, within CA265_RS08830 at 2208.946 kb on - strand, within CA265_RS08830 at 2208.975 kb on + strand, within CA265_RS08830 at 2208.976 kb on - strand, within CA265_RS08830 at 2209.001 kb on - strand, within CA265_RS08830 at 2209.021 kb on + strand, within CA265_RS08830 at 2209.021 kb on + strand, within CA265_RS08830 at 2209.054 kb on - strand, within CA265_RS08830 at 2209.064 kb on + strand, within CA265_RS08830 at 2209.153 kb on + strand, within CA265_RS08830 at 2209.249 kb on - strand, within CA265_RS08830 at 2209.275 kb on - strand at 2209.310 kb on + strand at 2209.310 kb on + strand at 2209.329 kb on + strand at 2209.444 kb on + strand at 2209.452 kb on + strand at 2209.664 kb on + strand, within CA265_RS08835
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.003 mg/ml remove 2,206,748 - CA265_RS08815 0.57 -0.2 2,206,775 - CA265_RS08815 0.64 +0.2 2,206,780 - CA265_RS08815 0.66 +1.0 2,206,857 + CA265_RS08815 0.87 -0.3 2,206,874 - +0.3 2,206,974 + -1.1 2,207,001 + -1.1 2,207,001 + -0.6 2,207,002 - -2.0 2,207,063 + CA265_RS08820 0.14 -1.3 2,207,064 - CA265_RS08820 0.14 -0.7 2,207,081 + CA265_RS08820 0.16 -1.7 2,207,081 + CA265_RS08820 0.16 -1.2 2,207,097 - CA265_RS08820 0.18 -0.9 2,207,167 - CA265_RS08820 0.27 -1.1 2,207,173 - CA265_RS08820 0.28 -0.7 2,207,279 + CA265_RS08820 0.41 -1.6 2,207,498 + CA265_RS08820 0.69 -1.6 2,207,532 + CA265_RS08820 0.73 -2.0 2,207,554 + CA265_RS08820 0.76 +0.2 2,207,587 + CA265_RS08820 0.80 -1.5 2,207,588 - CA265_RS08820 0.81 -0.8 2,207,588 - CA265_RS08820 0.81 -2.5 2,207,604 + CA265_RS08820 0.83 -1.7 2,207,834 + CA265_RS08825 0.10 -0.9 2,207,947 + CA265_RS08825 0.22 -2.1 2,208,011 + CA265_RS08825 0.29 -1.5 2,208,012 - +1.5 2,208,060 + CA265_RS08825 0.35 -3.6 2,208,060 + CA265_RS08825 0.35 -1.3 2,208,061 - CA265_RS08825 0.35 -0.9 2,208,069 - -0.5 2,208,119 - CA265_RS08825 0.41 +0.8 2,208,136 + CA265_RS08825 0.43 -0.4 2,208,137 - CA265_RS08825 0.43 -1.6 2,208,249 + CA265_RS08825 0.55 -1.0 2,208,260 + CA265_RS08825 0.56 -2.6 2,208,260 + CA265_RS08825 0.56 -1.2 2,208,260 + CA265_RS08825 0.56 -1.3 2,208,261 - CA265_RS08825 0.56 -1.0 2,208,263 + CA265_RS08825 0.56 -1.2 2,208,264 - CA265_RS08825 0.56 -0.7 2,208,294 - CA265_RS08825 0.60 -1.0 2,208,371 + CA265_RS08825 0.68 -1.1 2,208,382 + CA265_RS08825 0.69 -1.9 2,208,416 + CA265_RS08825 0.73 -1.6 2,208,416 + CA265_RS08825 0.73 -1.2 2,208,417 - CA265_RS08825 0.73 -1.7 2,208,417 - CA265_RS08825 0.73 -1.2 2,208,508 + CA265_RS08825 0.83 +1.1 2,208,551 + CA265_RS08825 0.87 -1.2 2,208,673 + +0.2 2,208,758 + -0.2 2,208,758 + -0.6 2,208,814 + +1.5 2,208,815 - -0.3 2,208,848 - CA265_RS08830 0.11 -1.0 2,208,892 + CA265_RS08830 0.20 +0.1 2,208,892 + CA265_RS08830 0.20 +0.1 2,208,893 - CA265_RS08830 0.20 +0.6 2,208,902 - CA265_RS08830 0.22 -0.2 2,208,904 - CA265_RS08830 0.22 -0.6 2,208,907 + CA265_RS08830 0.23 +0.1 2,208,910 + CA265_RS08830 0.23 -0.6 2,208,910 + CA265_RS08830 0.23 -0.1 2,208,920 + CA265_RS08830 0.25 -0.2 2,208,927 + CA265_RS08830 0.26 -0.9 2,208,927 + CA265_RS08830 0.26 +2.0 2,208,946 - CA265_RS08830 0.30 -0.3 2,208,975 + CA265_RS08830 0.36 +2.1 2,208,976 - CA265_RS08830 0.36 -1.2 2,209,001 - CA265_RS08830 0.41 -0.2 2,209,021 + CA265_RS08830 0.44 +0.2 2,209,021 + CA265_RS08830 0.44 +0.4 2,209,054 - CA265_RS08830 0.51 -0.7 2,209,064 + CA265_RS08830 0.53 +0.3 2,209,153 + CA265_RS08830 0.70 -0.2 2,209,249 - CA265_RS08830 0.88 +0.3 2,209,275 - -0.0 2,209,310 + -0.3 2,209,310 + +0.4 2,209,329 + +0.3 2,209,444 + +0.3 2,209,452 + -0.5 2,209,664 + CA265_RS08835 0.36 +0.5
Or see this region's nucleotide sequence