Experiment: R2A with Manganese (II) chloride tetrahydrate 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS16625 and LRK54_RS16630 are separated by 10 nucleotides LRK54_RS16630 and LRK54_RS16635 overlap by 4 nucleotides LRK54_RS16635 and LRK54_RS16640 are separated by 105 nucleotides
LRK54_RS16625: LRK54_RS16625 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 3,651,716 to 3,652,414
_RS16625
LRK54_RS16630: LRK54_RS16630 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 3,652,425 to 3,652,898
_RS16630
LRK54_RS16635: LRK54_RS16635 - magnesium transporter, at 3,652,895 to 3,654,289
_RS16635
LRK54_RS16640: LRK54_RS16640 - tRNA pseudouridine(13) synthase TruD, at 3,654,395 to 3,655,405
_RS16640
Position (kb)
3652
3653
3654
3655 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3652.905 kb on + strand at 3652.905 kb on + strand at 3652.905 kb on + strand at 3652.905 kb on + strand at 3652.905 kb on + strand at 3652.905 kb on + strand at 3652.905 kb on + strand at 3652.905 kb on + strand at 3652.905 kb on + strand at 3652.906 kb on - strand at 3652.906 kb on - strand at 3652.906 kb on - strand at 3652.906 kb on - strand at 3652.906 kb on - strand at 3652.906 kb on - strand at 3652.906 kb on - strand at 3652.906 kb on - strand at 3652.906 kb on - strand at 3652.906 kb on - strand at 3652.906 kb on - strand at 3652.906 kb on - strand at 3652.906 kb on - strand at 3652.906 kb on - strand at 3653.145 kb on + strand, within LRK54_RS16635 at 3653.145 kb on + strand, within LRK54_RS16635 at 3653.145 kb on + strand, within LRK54_RS16635 at 3653.146 kb on - strand, within LRK54_RS16635 at 3653.146 kb on - strand, within LRK54_RS16635 at 3653.146 kb on - strand, within LRK54_RS16635 at 3653.146 kb on - strand, within LRK54_RS16635 at 3653.146 kb on - strand, within LRK54_RS16635 at 3653.146 kb on - strand, within LRK54_RS16635 at 3653.146 kb on - strand, within LRK54_RS16635 at 3653.146 kb on - strand, within LRK54_RS16635 at 3653.330 kb on + strand, within LRK54_RS16635 at 3653.330 kb on + strand, within LRK54_RS16635 at 3653.331 kb on - strand, within LRK54_RS16635 at 3653.331 kb on - strand, within LRK54_RS16635 at 3653.591 kb on + strand, within LRK54_RS16635 at 3653.591 kb on + strand, within LRK54_RS16635 at 3653.591 kb on + strand, within LRK54_RS16635 at 3653.591 kb on + strand, within LRK54_RS16635 at 3653.591 kb on + strand, within LRK54_RS16635 at 3653.591 kb on + strand, within LRK54_RS16635 at 3653.591 kb on + strand, within LRK54_RS16635 at 3653.591 kb on + strand, within LRK54_RS16635 at 3653.591 kb on + strand, within LRK54_RS16635 at 3653.592 kb on - strand, within LRK54_RS16635 at 3653.592 kb on - strand, within LRK54_RS16635 at 3653.592 kb on - strand, within LRK54_RS16635 at 3653.592 kb on - strand, within LRK54_RS16635 at 3653.592 kb on - strand, within LRK54_RS16635 at 3653.592 kb on - strand, within LRK54_RS16635 at 3653.592 kb on - strand, within LRK54_RS16635 at 3653.592 kb on - strand, within LRK54_RS16635 at 3653.618 kb on + strand, within LRK54_RS16635 at 3653.618 kb on + strand, within LRK54_RS16635 at 3653.619 kb on - strand, within LRK54_RS16635 at 3653.772 kb on + strand, within LRK54_RS16635 at 3654.003 kb on + strand, within LRK54_RS16635 at 3654.003 kb on + strand, within LRK54_RS16635 at 3654.004 kb on - strand, within LRK54_RS16635 at 3654.083 kb on + strand, within LRK54_RS16635 at 3654.083 kb on + strand, within LRK54_RS16635 at 3654.083 kb on + strand, within LRK54_RS16635 at 3654.083 kb on + strand, within LRK54_RS16635 at 3654.084 kb on - strand, within LRK54_RS16635 at 3654.084 kb on - strand, within LRK54_RS16635 at 3654.084 kb on - strand, within LRK54_RS16635 at 3654.216 kb on + strand at 3654.216 kb on + strand at 3654.216 kb on + strand at 3654.216 kb on + strand at 3654.216 kb on + strand at 3654.216 kb on + strand at 3654.216 kb on + strand at 3654.217 kb on - strand at 3654.217 kb on - strand at 3654.217 kb on - strand at 3654.217 kb on - strand at 3654.217 kb on - strand at 3654.217 kb on - strand at 3654.287 kb on + strand at 3654.287 kb on + strand at 3654.287 kb on + strand at 3654.287 kb on + strand at 3654.287 kb on + strand at 3654.287 kb on + strand at 3654.287 kb on + strand at 3654.287 kb on + strand at 3654.287 kb on + strand at 3654.287 kb on + strand at 3654.287 kb on + strand at 3654.287 kb on + strand at 3654.287 kb on + strand at 3654.287 kb on + strand at 3654.287 kb on + strand at 3654.287 kb on + strand at 3654.287 kb on + strand at 3654.288 kb on - strand at 3654.288 kb on - strand at 3654.288 kb on - strand at 3654.288 kb on - strand at 3654.288 kb on - strand at 3654.288 kb on - strand at 3654.288 kb on - strand at 3654.288 kb on - strand at 3654.288 kb on - strand at 3654.288 kb on - strand at 3654.288 kb on - strand at 3654.288 kb on - strand at 3654.288 kb on - strand at 3654.288 kb on - strand at 3654.288 kb on - strand at 3654.319 kb on + strand at 3654.319 kb on + strand at 3654.319 kb on + strand at 3654.319 kb on + strand at 3654.319 kb on + strand at 3654.319 kb on + strand at 3654.320 kb on - strand at 3654.320 kb on - strand at 3654.320 kb on - strand at 3654.320 kb on - strand at 3654.320 kb on - strand at 3654.320 kb on - strand at 3654.320 kb on - strand at 3654.320 kb on - strand at 3654.320 kb on - strand at 3654.320 kb on - strand at 3654.320 kb on - strand at 3654.320 kb on - strand at 3654.323 kb on + strand at 3654.323 kb on + strand at 3654.323 kb on + strand at 3654.323 kb on + strand at 3654.323 kb on + strand at 3654.323 kb on + strand at 3654.323 kb on + strand at 3654.323 kb on + strand at 3654.323 kb on + strand at 3654.324 kb on - strand at 3654.324 kb on - strand at 3654.324 kb on - strand at 3654.324 kb on - strand at 3654.324 kb on - strand at 3654.324 kb on - strand at 3654.324 kb on - strand at 3654.324 kb on - strand at 3654.324 kb on - strand at 3654.324 kb on - strand at 3654.370 kb on + strand at 3654.371 kb on - strand at 3654.371 kb on - strand at 3654.371 kb on - strand at 3654.488 kb on + strand at 3654.488 kb on + strand at 3654.548 kb on - strand, within LRK54_RS16640 at 3654.548 kb on - strand, within LRK54_RS16640 at 3654.548 kb on - strand, within LRK54_RS16640 at 3654.608 kb on + strand, within LRK54_RS16640 at 3654.608 kb on + strand, within LRK54_RS16640 at 3654.608 kb on + strand, within LRK54_RS16640 at 3654.609 kb on - strand, within LRK54_RS16640 at 3654.863 kb on + strand, within LRK54_RS16640 at 3654.863 kb on + strand, within LRK54_RS16640 at 3654.863 kb on + strand, within LRK54_RS16640 at 3654.863 kb on + strand, within LRK54_RS16640 at 3654.863 kb on + strand, within LRK54_RS16640 at 3654.863 kb on + strand, within LRK54_RS16640 at 3654.863 kb on + strand, within LRK54_RS16640 at 3654.864 kb on - strand, within LRK54_RS16640 at 3654.864 kb on - strand, within LRK54_RS16640 at 3654.864 kb on - strand, within LRK54_RS16640 at 3654.864 kb on - strand, within LRK54_RS16640 at 3654.864 kb on - strand, within LRK54_RS16640 at 3654.864 kb on - strand, within LRK54_RS16640 at 3654.864 kb on - strand, within LRK54_RS16640 at 3654.864 kb on - strand, within LRK54_RS16640 at 3654.864 kb on - strand, within LRK54_RS16640
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Manganese (II) chloride tetrahydrate 2.5 mM remove 3,652,905 + +0.9 3,652,905 + +0.2 3,652,905 + +1.0 3,652,905 + +0.5 3,652,905 + -1.6 3,652,905 + -1.3 3,652,905 + +1.4 3,652,905 + +0.4 3,652,905 + -1.8 3,652,906 - -0.2 3,652,906 - +0.0 3,652,906 - -2.1 3,652,906 - +0.7 3,652,906 - +1.5 3,652,906 - +0.1 3,652,906 - +0.8 3,652,906 - +0.3 3,652,906 - +0.1 3,652,906 - +0.6 3,652,906 - -0.1 3,652,906 - +0.2 3,652,906 - -0.5 3,652,906 - -0.1 3,653,145 + LRK54_RS16635 0.18 -0.2 3,653,145 + LRK54_RS16635 0.18 -2.2 3,653,145 + LRK54_RS16635 0.18 -3.9 3,653,146 - LRK54_RS16635 0.18 -0.6 3,653,146 - LRK54_RS16635 0.18 +0.8 3,653,146 - LRK54_RS16635 0.18 -0.4 3,653,146 - LRK54_RS16635 0.18 +1.4 3,653,146 - LRK54_RS16635 0.18 +0.7 3,653,146 - LRK54_RS16635 0.18 +0.8 3,653,146 - LRK54_RS16635 0.18 +1.4 3,653,146 - LRK54_RS16635 0.18 -2.3 3,653,330 + LRK54_RS16635 0.31 +1.8 3,653,330 + LRK54_RS16635 0.31 -1.3 3,653,331 - LRK54_RS16635 0.31 +1.2 3,653,331 - LRK54_RS16635 0.31 +0.9 3,653,591 + LRK54_RS16635 0.50 +1.0 3,653,591 + LRK54_RS16635 0.50 +2.4 3,653,591 + LRK54_RS16635 0.50 +0.5 3,653,591 + LRK54_RS16635 0.50 -0.6 3,653,591 + LRK54_RS16635 0.50 +0.9 3,653,591 + LRK54_RS16635 0.50 +1.1 3,653,591 + LRK54_RS16635 0.50 +1.0 3,653,591 + LRK54_RS16635 0.50 +1.7 3,653,591 + LRK54_RS16635 0.50 +1.3 3,653,592 - LRK54_RS16635 0.50 +1.1 3,653,592 - LRK54_RS16635 0.50 +1.7 3,653,592 - LRK54_RS16635 0.50 +1.3 3,653,592 - LRK54_RS16635 0.50 +1.7 3,653,592 - LRK54_RS16635 0.50 +0.0 3,653,592 - LRK54_RS16635 0.50 +1.5 3,653,592 - LRK54_RS16635 0.50 +2.0 3,653,592 - LRK54_RS16635 0.50 +1.3 3,653,618 + LRK54_RS16635 0.52 +1.3 3,653,618 + LRK54_RS16635 0.52 +1.5 3,653,619 - LRK54_RS16635 0.52 +0.8 3,653,772 + LRK54_RS16635 0.63 +2.2 3,654,003 + LRK54_RS16635 0.79 +0.7 3,654,003 + LRK54_RS16635 0.79 +0.5 3,654,004 - LRK54_RS16635 0.79 +1.7 3,654,083 + LRK54_RS16635 0.85 +0.8 3,654,083 + LRK54_RS16635 0.85 +0.3 3,654,083 + LRK54_RS16635 0.85 +1.3 3,654,083 + LRK54_RS16635 0.85 -0.3 3,654,084 - LRK54_RS16635 0.85 +1.1 3,654,084 - LRK54_RS16635 0.85 +1.5 3,654,084 - LRK54_RS16635 0.85 +2.0 3,654,216 + +1.3 3,654,216 + +1.0 3,654,216 + +1.1 3,654,216 + +0.8 3,654,216 + +1.6 3,654,216 + +0.5 3,654,216 + +1.1 3,654,217 - +1.6 3,654,217 - +1.0 3,654,217 - +0.8 3,654,217 - +1.8 3,654,217 - +0.9 3,654,217 - +0.4 3,654,287 + +0.4 3,654,287 + +0.1 3,654,287 + -0.9 3,654,287 + -0.5 3,654,287 + -0.0 3,654,287 + -0.7 3,654,287 + +0.5 3,654,287 + -0.2 3,654,287 + -0.4 3,654,287 + -1.2 3,654,287 + -0.3 3,654,287 + -0.4 3,654,287 + -1.2 3,654,287 + +0.1 3,654,287 + -0.1 3,654,287 + +0.0 3,654,287 + +0.3 3,654,288 - +0.6 3,654,288 - +1.0 3,654,288 - +3.0 3,654,288 - +2.3 3,654,288 - +0.1 3,654,288 - +1.6 3,654,288 - +1.2 3,654,288 - -0.3 3,654,288 - +0.7 3,654,288 - +0.0 3,654,288 - -0.4 3,654,288 - -0.7 3,654,288 - +0.3 3,654,288 - +0.4 3,654,288 - +0.2 3,654,319 + +0.4 3,654,319 + -0.7 3,654,319 + -0.1 3,654,319 + -0.1 3,654,319 + -0.2 3,654,319 + -0.9 3,654,320 - +1.0 3,654,320 - +0.8 3,654,320 - -0.5 3,654,320 - +0.8 3,654,320 - +1.5 3,654,320 - -0.2 3,654,320 - +0.5 3,654,320 - +1.5 3,654,320 - -0.3 3,654,320 - +0.2 3,654,320 - +0.7 3,654,320 - +0.4 3,654,323 + -0.5 3,654,323 + +0.3 3,654,323 + -1.3 3,654,323 + -0.3 3,654,323 + +0.7 3,654,323 + +0.5 3,654,323 + -0.3 3,654,323 + -0.6 3,654,323 + -0.1 3,654,324 - +1.0 3,654,324 - +0.1 3,654,324 - +0.1 3,654,324 - +1.3 3,654,324 - +0.6 3,654,324 - +1.5 3,654,324 - +0.4 3,654,324 - +1.5 3,654,324 - +2.3 3,654,324 - +0.9 3,654,370 + -0.0 3,654,371 - +0.1 3,654,371 - +1.3 3,654,371 - +0.5 3,654,488 + +0.2 3,654,488 + +0.7 3,654,548 - LRK54_RS16640 0.15 +0.1 3,654,548 - LRK54_RS16640 0.15 +0.5 3,654,548 - LRK54_RS16640 0.15 -0.4 3,654,608 + LRK54_RS16640 0.21 +0.3 3,654,608 + LRK54_RS16640 0.21 +0.5 3,654,608 + LRK54_RS16640 0.21 -1.2 3,654,609 - LRK54_RS16640 0.21 +0.4 3,654,863 + LRK54_RS16640 0.46 +0.8 3,654,863 + LRK54_RS16640 0.46 +0.0 3,654,863 + LRK54_RS16640 0.46 +0.5 3,654,863 + LRK54_RS16640 0.46 +0.2 3,654,863 + LRK54_RS16640 0.46 -0.5 3,654,863 + LRK54_RS16640 0.46 -1.2 3,654,863 + LRK54_RS16640 0.46 -0.9 3,654,864 - LRK54_RS16640 0.46 +0.7 3,654,864 - LRK54_RS16640 0.46 -0.2 3,654,864 - LRK54_RS16640 0.46 +0.7 3,654,864 - LRK54_RS16640 0.46 -0.4 3,654,864 - LRK54_RS16640 0.46 +0.1 3,654,864 - LRK54_RS16640 0.46 +2.4 3,654,864 - LRK54_RS16640 0.46 +0.6 3,654,864 - LRK54_RS16640 0.46 -0.0 3,654,864 - LRK54_RS16640 0.46 -1.2
Or see this region's nucleotide sequence