Experiment: D-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1247 and Pf6N2E2_1248 are separated by 285 nucleotides Pf6N2E2_1248 and Pf6N2E2_1249 are separated by 145 nucleotides Pf6N2E2_1249 and Pf6N2E2_1250 are separated by 144 nucleotides
Pf6N2E2_1247 - hypothetical protein, at 1,459,263 to 1,459,448
_1247
Pf6N2E2_1248 - MutT/nudix family protein, at 1,459,734 to 1,460,096
_1248
Pf6N2E2_1249 - Transcriptional regulator, AraC family, at 1,460,242 to 1,461,165
_1249
Pf6N2E2_1250 - Nitric-oxide reductase (EC 1.7.99.7), quinol-dependent, at 1,461,310 to 1,463,583
_1250
Position (kb)
1460
1461
1462 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1459.282 kb on + strand, within Pf6N2E2_1247 at 1459.282 kb on + strand, within Pf6N2E2_1247 at 1459.301 kb on - strand, within Pf6N2E2_1247 at 1459.301 kb on - strand, within Pf6N2E2_1247 at 1459.362 kb on + strand, within Pf6N2E2_1247 at 1459.362 kb on + strand, within Pf6N2E2_1247 at 1459.362 kb on + strand, within Pf6N2E2_1247 at 1459.362 kb on + strand, within Pf6N2E2_1247 at 1459.362 kb on + strand, within Pf6N2E2_1247 at 1459.363 kb on - strand, within Pf6N2E2_1247 at 1459.547 kb on - strand at 1459.547 kb on - strand at 1459.553 kb on - strand at 1459.593 kb on + strand at 1459.594 kb on - strand at 1459.594 kb on - strand at 1459.632 kb on + strand at 1459.652 kb on + strand at 1459.694 kb on + strand at 1459.694 kb on + strand at 1459.695 kb on - strand at 1459.695 kb on - strand at 1459.696 kb on + strand at 1459.696 kb on + strand at 1459.696 kb on + strand at 1459.697 kb on - strand at 1459.706 kb on + strand at 1459.706 kb on + strand at 1459.859 kb on + strand, within Pf6N2E2_1248 at 1459.860 kb on - strand, within Pf6N2E2_1248 at 1459.919 kb on + strand, within Pf6N2E2_1248 at 1459.919 kb on + strand, within Pf6N2E2_1248 at 1459.919 kb on + strand at 1460.094 kb on + strand at 1460.094 kb on + strand at 1460.094 kb on + strand at 1460.094 kb on + strand at 1460.094 kb on + strand at 1460.094 kb on + strand at 1460.124 kb on - strand at 1460.166 kb on - strand at 1460.220 kb on + strand at 1460.221 kb on - strand at 1460.221 kb on - strand at 1460.399 kb on + strand, within Pf6N2E2_1249 at 1460.400 kb on - strand, within Pf6N2E2_1249 at 1460.432 kb on + strand, within Pf6N2E2_1249 at 1460.495 kb on + strand, within Pf6N2E2_1249 at 1460.552 kb on + strand, within Pf6N2E2_1249 at 1460.552 kb on + strand, within Pf6N2E2_1249 at 1460.634 kb on + strand, within Pf6N2E2_1249 at 1460.635 kb on - strand, within Pf6N2E2_1249 at 1460.963 kb on + strand, within Pf6N2E2_1249 at 1461.149 kb on + strand at 1461.149 kb on + strand at 1461.149 kb on + strand at 1461.190 kb on + strand at 1461.279 kb on + strand at 1461.312 kb on - strand at 1461.312 kb on - strand at 1461.519 kb on - strand at 1461.519 kb on - strand at 1461.639 kb on - strand, within Pf6N2E2_1250 at 1461.737 kb on + strand, within Pf6N2E2_1250 at 1461.740 kb on + strand, within Pf6N2E2_1250 at 1461.740 kb on + strand, within Pf6N2E2_1250 at 1461.740 kb on + strand, within Pf6N2E2_1250 at 1461.741 kb on - strand, within Pf6N2E2_1250 at 1461.975 kb on - strand, within Pf6N2E2_1250 at 1461.975 kb on - strand, within Pf6N2E2_1250 at 1461.975 kb on - strand, within Pf6N2E2_1250 at 1461.975 kb on - strand, within Pf6N2E2_1250 at 1461.975 kb on - strand, within Pf6N2E2_1250 at 1461.975 kb on - strand, within Pf6N2E2_1250 at 1462.074 kb on + strand, within Pf6N2E2_1250 at 1462.132 kb on + strand, within Pf6N2E2_1250 at 1462.133 kb on - strand, within Pf6N2E2_1250 at 1462.133 kb on - strand, within Pf6N2E2_1250 at 1462.133 kb on - strand, within Pf6N2E2_1250 at 1462.133 kb on - strand, within Pf6N2E2_1250 at 1462.157 kb on + strand, within Pf6N2E2_1250
Per-strain Table
Position Strand Gene LocusTag Fraction D-Lactate (C) remove 1,459,282 + Pf6N2E2_1247 0.10 +0.1 1,459,282 + Pf6N2E2_1247 0.10 -0.1 1,459,301 - Pf6N2E2_1247 0.20 -0.5 1,459,301 - Pf6N2E2_1247 0.20 -0.4 1,459,362 + Pf6N2E2_1247 0.53 +0.4 1,459,362 + Pf6N2E2_1247 0.53 +0.7 1,459,362 + Pf6N2E2_1247 0.53 +0.9 1,459,362 + Pf6N2E2_1247 0.53 -1.0 1,459,362 + Pf6N2E2_1247 0.53 +0.3 1,459,363 - Pf6N2E2_1247 0.54 +0.5 1,459,547 - -1.8 1,459,547 - -0.5 1,459,553 - +1.0 1,459,593 + +0.3 1,459,594 - +0.3 1,459,594 - -0.0 1,459,632 + +0.0 1,459,652 + +0.1 1,459,694 + -0.3 1,459,694 + +0.4 1,459,695 - +0.0 1,459,695 - -0.3 1,459,696 + -1.4 1,459,696 + -0.9 1,459,696 + +0.2 1,459,697 - +0.7 1,459,706 + +0.3 1,459,706 + +0.4 1,459,859 + Pf6N2E2_1248 0.34 -0.0 1,459,860 - Pf6N2E2_1248 0.35 +0.9 1,459,919 + Pf6N2E2_1248 0.51 +0.4 1,459,919 + Pf6N2E2_1248 0.51 +0.6 1,459,919 + +3.0 1,460,094 + +0.7 1,460,094 + -0.2 1,460,094 + +0.2 1,460,094 + +0.6 1,460,094 + -0.1 1,460,094 + +0.3 1,460,124 - +0.3 1,460,166 - -0.0 1,460,220 + -0.6 1,460,221 - +1.4 1,460,221 - -0.0 1,460,399 + Pf6N2E2_1249 0.17 -0.2 1,460,400 - Pf6N2E2_1249 0.17 -0.4 1,460,432 + Pf6N2E2_1249 0.21 -0.4 1,460,495 + Pf6N2E2_1249 0.27 -1.0 1,460,552 + Pf6N2E2_1249 0.34 -1.8 1,460,552 + Pf6N2E2_1249 0.34 -1.0 1,460,634 + Pf6N2E2_1249 0.42 +0.2 1,460,635 - Pf6N2E2_1249 0.43 -0.4 1,460,963 + Pf6N2E2_1249 0.78 +0.8 1,461,149 + -0.0 1,461,149 + -0.1 1,461,149 + -0.5 1,461,190 + -0.4 1,461,279 + +0.4 1,461,312 - +0.3 1,461,312 - +1.4 1,461,519 - -0.6 1,461,519 - +1.0 1,461,639 - Pf6N2E2_1250 0.14 -0.2 1,461,737 + Pf6N2E2_1250 0.19 +0.3 1,461,740 + Pf6N2E2_1250 0.19 +1.4 1,461,740 + Pf6N2E2_1250 0.19 -0.1 1,461,740 + Pf6N2E2_1250 0.19 +1.8 1,461,741 - Pf6N2E2_1250 0.19 -0.1 1,461,975 - Pf6N2E2_1250 0.29 -0.7 1,461,975 - Pf6N2E2_1250 0.29 -0.6 1,461,975 - Pf6N2E2_1250 0.29 +1.1 1,461,975 - Pf6N2E2_1250 0.29 -0.5 1,461,975 - Pf6N2E2_1250 0.29 +0.6 1,461,975 - Pf6N2E2_1250 0.29 +0.1 1,462,074 + Pf6N2E2_1250 0.34 +0.8 1,462,132 + Pf6N2E2_1250 0.36 +1.3 1,462,133 - Pf6N2E2_1250 0.36 +0.5 1,462,133 - Pf6N2E2_1250 0.36 +0.5 1,462,133 - Pf6N2E2_1250 0.36 -0.5 1,462,133 - Pf6N2E2_1250 0.36 +0.5 1,462,157 + Pf6N2E2_1250 0.37 +0.3
Or see this region's nucleotide sequence