Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS22465

Experiment: D-Lactate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS22460 and BPHYT_RS22465 are separated by 291 nucleotidesBPHYT_RS22465 and BPHYT_RS22470 overlap by 4 nucleotidesBPHYT_RS22470 and BPHYT_RS22475 are separated by 169 nucleotides BPHYT_RS22460: BPHYT_RS22460 - phosphonate ABC transporter ATP-binidng protein, at 594,255 to 595,160 _RS22460 BPHYT_RS22465: BPHYT_RS22465 - zinc carboxypeptidase-like protein, at 595,452 to 596,486 _RS22465 BPHYT_RS22470: BPHYT_RS22470 - hydrolase, at 596,483 to 597,283 _RS22470 BPHYT_RS22475: BPHYT_RS22475 - NAD-glutamate dehydrogenase, at 597,453 to 602,294 _RS22475 Position (kb) 595 596 597Strain fitness (log2 ratio) -1 0 1at 595.165 kb on - strandat 595.268 kb on - strandat 595.268 kb on - strandat 595.393 kb on + strandat 595.838 kb on + strand, within BPHYT_RS22465at 596.145 kb on + strand, within BPHYT_RS22465at 596.172 kb on + strand, within BPHYT_RS22465at 596.176 kb on + strand, within BPHYT_RS22465at 596.467 kb on - strandat 596.468 kb on + strandat 596.482 kb on + strandat 596.816 kb on - strand, within BPHYT_RS22470at 597.325 kb on + strandat 597.371 kb on + strandat 597.371 kb on + strandat 597.372 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Lactate (C)
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595,165 - -0.2
595,268 - -0.3
595,268 - -0.4
595,393 + +0.1
595,838 + BPHYT_RS22465 0.37 +0.3
596,145 + BPHYT_RS22465 0.67 -0.2
596,172 + BPHYT_RS22465 0.70 -0.3
596,176 + BPHYT_RS22465 0.70 +0.8
596,467 - +0.7
596,468 + -0.4
596,482 + -0.2
596,816 - BPHYT_RS22470 0.42 +0.1
597,325 + -0.2
597,371 + +1.3
597,371 + -0.4
597,372 - +0.1

Or see this region's nucleotide sequence