Experiment: R2A with Zinc 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33575 and RR42_RS33580 are separated by 50 nucleotides RR42_RS33580 and RR42_RS33585 are separated by 290 nucleotides
RR42_RS33575: RR42_RS33575 - hypothetical protein, at 2,961,252 to 2,962,085
_RS33575
RR42_RS33580: RR42_RS33580 - 3-hydroxyacyl-CoA dehydrogenase, at 2,962,136 to 2,963,050
_RS33580
RR42_RS33585: RR42_RS33585 - 3-keto-5-aminohexanoate cleavage protein, at 2,963,341 to 2,964,273
_RS33585
Position (kb)
2962
2963
2964 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2961.182 kb on + strand at 2961.182 kb on + strand at 2961.540 kb on - strand, within RR42_RS33575 at 2961.679 kb on + strand, within RR42_RS33575 at 2961.680 kb on - strand, within RR42_RS33575 at 2961.834 kb on - strand, within RR42_RS33575 at 2962.122 kb on - strand at 2962.122 kb on - strand at 2962.129 kb on - strand at 2962.129 kb on - strand at 2962.137 kb on + strand at 2962.137 kb on + strand at 2962.138 kb on - strand at 2962.164 kb on - strand at 2962.179 kb on + strand at 2962.230 kb on + strand, within RR42_RS33580 at 2962.231 kb on - strand, within RR42_RS33580 at 2962.231 kb on - strand, within RR42_RS33580 at 2962.231 kb on - strand, within RR42_RS33580 at 2962.288 kb on + strand, within RR42_RS33580 at 2962.289 kb on - strand, within RR42_RS33580 at 2962.309 kb on + strand, within RR42_RS33580 at 2962.310 kb on - strand, within RR42_RS33580 at 2962.541 kb on + strand, within RR42_RS33580 at 2962.715 kb on + strand, within RR42_RS33580 at 2962.715 kb on + strand, within RR42_RS33580 at 2962.755 kb on - strand, within RR42_RS33580 at 2962.839 kb on - strand, within RR42_RS33580 at 2962.997 kb on + strand at 2962.997 kb on + strand at 2963.044 kb on + strand at 2963.044 kb on + strand at 2963.044 kb on + strand at 2963.044 kb on + strand at 2963.044 kb on + strand at 2963.044 kb on + strand at 2963.044 kb on + strand at 2963.045 kb on - strand at 2963.045 kb on - strand at 2963.045 kb on - strand at 2963.045 kb on - strand at 2963.045 kb on - strand at 2963.045 kb on - strand at 2963.045 kb on - strand at 2963.411 kb on - strand at 2963.538 kb on + strand, within RR42_RS33585 at 2963.538 kb on + strand, within RR42_RS33585 at 2963.749 kb on + strand, within RR42_RS33585 at 2963.749 kb on + strand, within RR42_RS33585 at 2963.749 kb on + strand, within RR42_RS33585 at 2963.749 kb on + strand, within RR42_RS33585 at 2963.750 kb on - strand, within RR42_RS33585 at 2963.769 kb on + strand, within RR42_RS33585 at 2963.769 kb on + strand, within RR42_RS33585 at 2963.770 kb on - strand, within RR42_RS33585 at 2963.770 kb on - strand, within RR42_RS33585 at 2963.784 kb on + strand, within RR42_RS33585 at 2963.785 kb on - strand, within RR42_RS33585 at 2963.785 kb on - strand, within RR42_RS33585 at 2963.857 kb on + strand, within RR42_RS33585 at 2963.857 kb on + strand, within RR42_RS33585 at 2963.858 kb on - strand, within RR42_RS33585 at 2963.858 kb on - strand, within RR42_RS33585 at 2963.858 kb on - strand, within RR42_RS33585 at 2963.864 kb on + strand, within RR42_RS33585 at 2963.864 kb on + strand, within RR42_RS33585 at 2963.864 kb on + strand, within RR42_RS33585 at 2963.864 kb on + strand, within RR42_RS33585 at 2963.875 kb on + strand, within RR42_RS33585 at 2964.000 kb on + strand, within RR42_RS33585 at 2964.022 kb on + strand, within RR42_RS33585 at 2964.023 kb on - strand, within RR42_RS33585 at 2964.023 kb on - strand, within RR42_RS33585 at 2964.025 kb on + strand, within RR42_RS33585 at 2964.025 kb on + strand, within RR42_RS33585 at 2964.026 kb on - strand, within RR42_RS33585
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Zinc 0.1 mM remove 2,961,182 + +1.1 2,961,182 + +1.2 2,961,540 - RR42_RS33575 0.35 +0.1 2,961,679 + RR42_RS33575 0.51 +0.2 2,961,680 - RR42_RS33575 0.51 +0.2 2,961,834 - RR42_RS33575 0.70 -0.9 2,962,122 - -0.2 2,962,122 - -0.4 2,962,129 - +0.2 2,962,129 - -1.1 2,962,137 + -0.2 2,962,137 + -0.4 2,962,138 - -0.0 2,962,164 - +0.4 2,962,179 + -0.2 2,962,230 + RR42_RS33580 0.10 +0.1 2,962,231 - RR42_RS33580 0.10 +0.3 2,962,231 - RR42_RS33580 0.10 -0.1 2,962,231 - RR42_RS33580 0.10 -0.7 2,962,288 + RR42_RS33580 0.17 -0.4 2,962,289 - RR42_RS33580 0.17 -1.1 2,962,309 + RR42_RS33580 0.19 +2.2 2,962,310 - RR42_RS33580 0.19 +0.2 2,962,541 + RR42_RS33580 0.44 -0.1 2,962,715 + RR42_RS33580 0.63 +1.1 2,962,715 + RR42_RS33580 0.63 +0.6 2,962,755 - RR42_RS33580 0.68 -0.6 2,962,839 - RR42_RS33580 0.77 -0.4 2,962,997 + +0.1 2,962,997 + -1.2 2,963,044 + -1.1 2,963,044 + -1.9 2,963,044 + -0.1 2,963,044 + -1.4 2,963,044 + -1.4 2,963,044 + -1.3 2,963,044 + -1.0 2,963,045 - -0.4 2,963,045 - -0.1 2,963,045 - -0.0 2,963,045 - +2.0 2,963,045 - +0.1 2,963,045 - +0.0 2,963,045 - -0.5 2,963,411 - +0.0 2,963,538 + RR42_RS33585 0.21 -0.9 2,963,538 + RR42_RS33585 0.21 -0.7 2,963,749 + RR42_RS33585 0.44 -0.4 2,963,749 + RR42_RS33585 0.44 -0.4 2,963,749 + RR42_RS33585 0.44 -0.8 2,963,749 + RR42_RS33585 0.44 -0.2 2,963,750 - RR42_RS33585 0.44 +1.3 2,963,769 + RR42_RS33585 0.46 +1.0 2,963,769 + RR42_RS33585 0.46 +1.4 2,963,770 - RR42_RS33585 0.46 -0.8 2,963,770 - RR42_RS33585 0.46 -0.1 2,963,784 + RR42_RS33585 0.47 +0.4 2,963,785 - RR42_RS33585 0.48 +0.4 2,963,785 - RR42_RS33585 0.48 +0.8 2,963,857 + RR42_RS33585 0.55 +0.0 2,963,857 + RR42_RS33585 0.55 +2.5 2,963,858 - RR42_RS33585 0.55 +0.0 2,963,858 - RR42_RS33585 0.55 +0.3 2,963,858 - RR42_RS33585 0.55 +0.4 2,963,864 + RR42_RS33585 0.56 +0.3 2,963,864 + RR42_RS33585 0.56 +0.6 2,963,864 + RR42_RS33585 0.56 -0.6 2,963,864 + RR42_RS33585 0.56 +0.3 2,963,875 + RR42_RS33585 0.57 +1.9 2,964,000 + RR42_RS33585 0.71 +0.2 2,964,022 + RR42_RS33585 0.73 -0.8 2,964,023 - RR42_RS33585 0.73 +0.6 2,964,023 - RR42_RS33585 0.73 +1.4 2,964,025 + RR42_RS33585 0.73 -0.4 2,964,025 + RR42_RS33585 0.73 +0.2 2,964,026 - RR42_RS33585 0.73 -1.3
Or see this region's nucleotide sequence