Experiment: R2A with Zinc 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32625 and RR42_RS32630 are separated by 24 nucleotides RR42_RS32630 and RR42_RS32635 are separated by 20 nucleotides RR42_RS32635 and RR42_RS32640 are separated by 38 nucleotides
RR42_RS32625: RR42_RS32625 - 4-hyroxy-2-oxovalerate aldolase, at 2,717,513 to 2,718,562
_RS32625
RR42_RS32630: RR42_RS32630 - acetaldehyde dehydrogenase, at 2,718,587 to 2,719,531
_RS32630
RR42_RS32635: RR42_RS32635 - 4-oxalocrotonate tautomerase, at 2,719,552 to 2,719,743
_RS32635
RR42_RS32640: RR42_RS32640 - 4-oxalocrotonate decarboxylase, at 2,719,782 to 2,720,570
_RS32640
Position (kb)
2718
2719
2720 Strain fitness (log2 ratio)
-1
0
1
2 at 2717.612 kb on + strand at 2717.646 kb on + strand, within RR42_RS32625 at 2717.688 kb on + strand, within RR42_RS32625 at 2717.788 kb on - strand, within RR42_RS32625 at 2718.032 kb on - strand, within RR42_RS32625 at 2718.169 kb on + strand, within RR42_RS32625 at 2718.170 kb on - strand, within RR42_RS32625 at 2718.305 kb on + strand, within RR42_RS32625 at 2718.376 kb on - strand, within RR42_RS32625 at 2718.380 kb on - strand, within RR42_RS32625 at 2718.481 kb on - strand at 2718.530 kb on - strand at 2718.531 kb on + strand at 2718.532 kb on - strand at 2718.532 kb on - strand at 2718.666 kb on + strand at 2718.666 kb on + strand at 2718.666 kb on + strand at 2718.667 kb on - strand at 2718.748 kb on + strand, within RR42_RS32630 at 2718.816 kb on + strand, within RR42_RS32630 at 2718.816 kb on + strand, within RR42_RS32630 at 2718.816 kb on + strand, within RR42_RS32630 at 2718.817 kb on - strand, within RR42_RS32630 at 2718.817 kb on - strand, within RR42_RS32630 at 2719.072 kb on - strand, within RR42_RS32630 at 2719.141 kb on + strand, within RR42_RS32630 at 2719.141 kb on + strand, within RR42_RS32630 at 2719.141 kb on + strand, within RR42_RS32630 at 2719.142 kb on - strand, within RR42_RS32630 at 2719.142 kb on - strand, within RR42_RS32630 at 2719.194 kb on + strand, within RR42_RS32630 at 2719.194 kb on + strand, within RR42_RS32630
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Zinc 0.1 mM remove 2,717,612 + -1.6 2,717,646 + RR42_RS32625 0.13 +0.3 2,717,688 + RR42_RS32625 0.17 -0.8 2,717,788 - RR42_RS32625 0.26 +0.0 2,718,032 - RR42_RS32625 0.49 +2.0 2,718,169 + RR42_RS32625 0.62 +0.7 2,718,170 - RR42_RS32625 0.63 +0.7 2,718,305 + RR42_RS32625 0.75 -1.4 2,718,376 - RR42_RS32625 0.82 -1.2 2,718,380 - RR42_RS32625 0.83 +0.6 2,718,481 - +0.9 2,718,530 - +0.3 2,718,531 + +0.9 2,718,532 - +0.9 2,718,532 - +1.0 2,718,666 + -0.6 2,718,666 + +0.0 2,718,666 + -0.2 2,718,667 - +1.0 2,718,748 + RR42_RS32630 0.17 +0.6 2,718,816 + RR42_RS32630 0.24 -0.3 2,718,816 + RR42_RS32630 0.24 -0.7 2,718,816 + RR42_RS32630 0.24 -1.2 2,718,817 - RR42_RS32630 0.24 -0.6 2,718,817 - RR42_RS32630 0.24 +0.5 2,719,072 - RR42_RS32630 0.51 +0.1 2,719,141 + RR42_RS32630 0.59 +1.1 2,719,141 + RR42_RS32630 0.59 +2.1 2,719,141 + RR42_RS32630 0.59 -0.3 2,719,142 - RR42_RS32630 0.59 +0.5 2,719,142 - RR42_RS32630 0.59 +0.4 2,719,194 + RR42_RS32630 0.64 -0.8 2,719,194 + RR42_RS32630 0.64 +0.2
Or see this region's nucleotide sequence