Experiment: R2A with Zinc 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31240 and RR42_RS31245 overlap by 8 nucleotides RR42_RS31245 and RR42_RS31250 are separated by 6 nucleotides RR42_RS31250 and RR42_RS31255 are separated by 87 nucleotides RR42_RS31255 and RR42_RS31260 overlap by 14 nucleotides
RR42_RS31240: RR42_RS31240 - AMP-dependent synthetase, at 2,385,100 to 2,386,818
_RS31240
RR42_RS31245: RR42_RS31245 - membrane protein, at 2,386,811 to 2,387,359
_RS31245
RR42_RS31250: RR42_RS31250 - acyl carrier protein, at 2,387,366 to 2,387,623
_RS31250
RR42_RS31255: RR42_RS31255 - acyl carrier protein, at 2,387,711 to 2,387,986
_RS31255
RR42_RS31260: RR42_RS31260 - acyltransferase, at 2,387,973 to 2,388,749
_RS31260
Position (kb)
2386
2387
2388 Strain fitness (log2 ratio)
-2
-1
0
1 at 2386.274 kb on + strand, within RR42_RS31240 at 2386.275 kb on - strand, within RR42_RS31240 at 2386.275 kb on - strand, within RR42_RS31240 at 2386.275 kb on - strand, within RR42_RS31240 at 2386.420 kb on - strand, within RR42_RS31240 at 2386.653 kb on - strand at 2386.836 kb on - strand at 2386.887 kb on + strand, within RR42_RS31245 at 2386.905 kb on + strand, within RR42_RS31245 at 2387.301 kb on + strand, within RR42_RS31245 at 2387.301 kb on + strand, within RR42_RS31245 at 2387.317 kb on - strand at 2387.681 kb on - strand at 2387.681 kb on - strand at 2387.681 kb on - strand at 2388.016 kb on - strand at 2388.109 kb on + strand, within RR42_RS31260 at 2388.109 kb on + strand, within RR42_RS31260 at 2388.110 kb on - strand, within RR42_RS31260 at 2388.110 kb on - strand, within RR42_RS31260 at 2388.110 kb on - strand, within RR42_RS31260 at 2388.110 kb on - strand, within RR42_RS31260 at 2388.358 kb on + strand, within RR42_RS31260 at 2388.359 kb on - strand, within RR42_RS31260
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Zinc 0.1 mM remove 2,386,274 + RR42_RS31240 0.68 +0.4 2,386,275 - RR42_RS31240 0.68 -0.8 2,386,275 - RR42_RS31240 0.68 -2.3 2,386,275 - RR42_RS31240 0.68 +1.6 2,386,420 - RR42_RS31240 0.77 +0.5 2,386,653 - +0.1 2,386,836 - +0.5 2,386,887 + RR42_RS31245 0.14 -0.3 2,386,905 + RR42_RS31245 0.17 -1.1 2,387,301 + RR42_RS31245 0.89 -1.2 2,387,301 + RR42_RS31245 0.89 -0.1 2,387,317 - -0.2 2,387,681 - -0.3 2,387,681 - +0.1 2,387,681 - +0.1 2,388,016 - +1.0 2,388,109 + RR42_RS31260 0.18 -0.9 2,388,109 + RR42_RS31260 0.18 -0.1 2,388,110 - RR42_RS31260 0.18 -0.1 2,388,110 - RR42_RS31260 0.18 -1.1 2,388,110 - RR42_RS31260 0.18 -0.3 2,388,110 - RR42_RS31260 0.18 -0.4 2,388,358 + RR42_RS31260 0.50 -1.3 2,388,359 - RR42_RS31260 0.50 -0.3
Or see this region's nucleotide sequence