Experiment: R2A with Zinc 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS26130 and RR42_RS26135 are separated by 110 nucleotides RR42_RS26135 and RR42_RS26140 are separated by 339 nucleotides
RR42_RS26130: RR42_RS26130 - C4-dicarboxylate ABC transporter, at 1,216,778 to 1,218,088
_RS26130
RR42_RS26135: RR42_RS26135 - aspartate ammonia-lyase, at 1,218,199 to 1,219,626
_RS26135
RR42_RS26140: RR42_RS26140 - glutaminase, at 1,219,966 to 1,221,087
_RS26140
Position (kb)
1218
1219
1220 Strain fitness (log2 ratio)
-1
0
1
2 at 1217.425 kb on + strand, within RR42_RS26130 at 1217.426 kb on - strand, within RR42_RS26130 at 1217.970 kb on + strand at 1217.971 kb on - strand at 1217.971 kb on - strand at 1218.373 kb on - strand, within RR42_RS26135 at 1218.373 kb on - strand, within RR42_RS26135 at 1218.374 kb on + strand, within RR42_RS26135 at 1218.893 kb on + strand, within RR42_RS26135 at 1219.235 kb on + strand, within RR42_RS26135 at 1219.241 kb on + strand, within RR42_RS26135 at 1219.466 kb on + strand, within RR42_RS26135 at 1219.467 kb on - strand, within RR42_RS26135 at 1219.467 kb on - strand, within RR42_RS26135 at 1219.494 kb on - strand at 1219.557 kb on - strand at 1219.557 kb on - strand at 1219.560 kb on - strand at 1219.560 kb on - strand at 1219.669 kb on + strand at 1219.728 kb on + strand at 1219.819 kb on - strand at 1219.986 kb on + strand at 1219.986 kb on + strand at 1219.987 kb on - strand at 1219.987 kb on - strand at 1219.987 kb on - strand at 1219.987 kb on - strand at 1220.176 kb on + strand, within RR42_RS26140 at 1220.398 kb on + strand, within RR42_RS26140 at 1220.398 kb on + strand, within RR42_RS26140 at 1220.497 kb on + strand, within RR42_RS26140
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Zinc 0.1 mM remove 1,217,425 + RR42_RS26130 0.49 -1.2 1,217,426 - RR42_RS26130 0.49 +1.1 1,217,970 + +0.1 1,217,971 - -0.5 1,217,971 - +0.3 1,218,373 - RR42_RS26135 0.12 +0.0 1,218,373 - RR42_RS26135 0.12 -0.4 1,218,374 + RR42_RS26135 0.12 +0.4 1,218,893 + RR42_RS26135 0.49 -1.4 1,219,235 + RR42_RS26135 0.73 +0.1 1,219,241 + RR42_RS26135 0.73 -0.8 1,219,466 + RR42_RS26135 0.89 +0.7 1,219,467 - RR42_RS26135 0.89 -1.3 1,219,467 - RR42_RS26135 0.89 +1.6 1,219,494 - +0.9 1,219,557 - -0.4 1,219,557 - +1.9 1,219,560 - -0.3 1,219,560 - +0.1 1,219,669 + -1.1 1,219,728 + -0.9 1,219,819 - -0.6 1,219,986 + -1.3 1,219,986 + +0.2 1,219,987 - +0.7 1,219,987 - +1.6 1,219,987 - +0.8 1,219,987 - +0.9 1,220,176 + RR42_RS26140 0.19 +0.4 1,220,398 + RR42_RS26140 0.39 -0.4 1,220,398 + RR42_RS26140 0.39 +0.3 1,220,497 + RR42_RS26140 0.47 -1.5
Or see this region's nucleotide sequence