Experiment: R2A with Zinc 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03400 and RR42_RS03405 overlap by 8 nucleotides RR42_RS03405 and RR42_RS03410 are separated by 16 nucleotides RR42_RS03410 and RR42_RS03415 overlap by 7 nucleotides
RR42_RS03400: RR42_RS03400 - permease, at 756,703 to 757,596
_RS03400
RR42_RS03405: RR42_RS03405 - epoxyqueuosine reductase, at 757,589 to 758,809
_RS03405
RR42_RS03410: RR42_RS03410 - ATPase, at 758,826 to 759,338
_RS03410
RR42_RS03415: RR42_RS03415 - N-acetylmuramoyl-L-alanine amidase, at 759,332 to 760,837
_RS03415
Position (kb) 757
758
759 Strain fitness (log2 ratio) -2
-1
0
1 at 756.611 kb on + strand at 756.612 kb on - strand at 756.612 kb on - strand at 756.653 kb on - strand at 756.688 kb on - strand at 756.688 kb on - strand at 756.688 kb on - strand at 756.691 kb on + strand at 756.818 kb on - strand, within RR42_RS03400 at 756.940 kb on + strand, within RR42_RS03400 at 756.941 kb on - strand, within RR42_RS03400 at 757.018 kb on + strand, within RR42_RS03400 at 757.018 kb on + strand, within RR42_RS03400 at 757.019 kb on - strand, within RR42_RS03400 at 757.019 kb on - strand, within RR42_RS03400 at 757.019 kb on - strand, within RR42_RS03400 at 757.483 kb on + strand, within RR42_RS03400 at 757.483 kb on + strand, within RR42_RS03400 at 757.484 kb on - strand, within RR42_RS03400 at 757.484 kb on - strand, within RR42_RS03400 at 757.484 kb on - strand, within RR42_RS03400 at 757.484 kb on - strand, within RR42_RS03400 at 757.485 kb on + strand, within RR42_RS03400 at 757.486 kb on - strand, within RR42_RS03400 at 757.486 kb on - strand, within RR42_RS03400 at 757.625 kb on - strand at 757.947 kb on + strand, within RR42_RS03405 at 757.947 kb on + strand, within RR42_RS03405 at 757.947 kb on + strand, within RR42_RS03405 at 757.948 kb on - strand, within RR42_RS03405 at 758.013 kb on + strand, within RR42_RS03405 at 758.349 kb on + strand, within RR42_RS03405 at 758.452 kb on - strand, within RR42_RS03405 at 758.533 kb on + strand, within RR42_RS03405 at 758.535 kb on + strand, within RR42_RS03405 at 758.535 kb on + strand, within RR42_RS03405 at 758.535 kb on + strand, within RR42_RS03405 at 758.535 kb on + strand, within RR42_RS03405 at 758.535 kb on + strand, within RR42_RS03405 at 758.820 kb on + strand at 758.820 kb on + strand at 758.820 kb on + strand at 759.506 kb on + strand, within RR42_RS03415 at 759.506 kb on + strand, within RR42_RS03415 at 759.507 kb on - strand, within RR42_RS03415 at 759.656 kb on + strand, within RR42_RS03415 at 759.656 kb on + strand, within RR42_RS03415 at 759.657 kb on - strand, within RR42_RS03415 at 759.657 kb on - strand, within RR42_RS03415 at 759.745 kb on + strand, within RR42_RS03415 at 759.746 kb on - strand, within RR42_RS03415 at 759.794 kb on + strand, within RR42_RS03415
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Zinc 0.1 mM remove 756,611 + -1.8 756,612 - -0.6 756,612 - -1.6 756,653 - +0.6 756,688 - +0.3 756,688 - -1.1 756,688 - -0.0 756,691 + -1.2 756,818 - RR42_RS03400 0.13 +0.3 756,940 + RR42_RS03400 0.27 +0.6 756,941 - RR42_RS03400 0.27 +0.2 757,018 + RR42_RS03400 0.35 +0.8 757,018 + RR42_RS03400 0.35 +1.6 757,019 - RR42_RS03400 0.35 +0.9 757,019 - RR42_RS03400 0.35 +1.1 757,019 - RR42_RS03400 0.35 -0.0 757,483 + RR42_RS03400 0.87 -0.4 757,483 + RR42_RS03400 0.87 -0.5 757,484 - RR42_RS03400 0.87 +0.5 757,484 - RR42_RS03400 0.87 +1.2 757,484 - RR42_RS03400 0.87 +0.2 757,484 - RR42_RS03400 0.87 -0.5 757,485 + RR42_RS03400 0.87 -2.2 757,486 - RR42_RS03400 0.88 -0.8 757,486 - RR42_RS03400 0.88 +0.2 757,625 - -2.4 757,947 + RR42_RS03405 0.29 -1.2 757,947 + RR42_RS03405 0.29 +1.4 757,947 + RR42_RS03405 0.29 +0.1 757,948 - RR42_RS03405 0.29 -0.0 758,013 + RR42_RS03405 0.35 -0.2 758,349 + RR42_RS03405 0.62 +1.5 758,452 - RR42_RS03405 0.71 +0.3 758,533 + RR42_RS03405 0.77 +0.4 758,535 + RR42_RS03405 0.77 +0.2 758,535 + RR42_RS03405 0.77 +0.8 758,535 + RR42_RS03405 0.77 +0.6 758,535 + RR42_RS03405 0.77 -0.2 758,535 + RR42_RS03405 0.77 +0.4 758,820 + -0.7 758,820 + +0.4 758,820 + +1.0 759,506 + RR42_RS03415 0.12 -1.6 759,506 + RR42_RS03415 0.12 -0.8 759,507 - RR42_RS03415 0.12 -0.4 759,656 + RR42_RS03415 0.22 -2.0 759,656 + RR42_RS03415 0.22 -2.1 759,657 - RR42_RS03415 0.22 +0.5 759,657 - RR42_RS03415 0.22 -0.2 759,745 + RR42_RS03415 0.27 +0.4 759,746 - RR42_RS03415 0.27 -0.0 759,794 + RR42_RS03415 0.31 -1.0
Or see this region's nucleotide sequence