Experiment: R2A with Zinc 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02885 and RR42_RS02890 are separated by 60 nucleotides RR42_RS02890 and RR42_RS02895 are separated by 327 nucleotides RR42_RS02895 and RR42_RS02900 are separated by 117 nucleotides
RR42_RS02885: RR42_RS02885 - acetylornithine deacetylase, at 644,051 to 645,613
_RS02885
RR42_RS02890: RR42_RS02890 - 4-carboxymuconolactone decarboxylase, at 645,674 to 646,093
_RS02890
RR42_RS02895: RR42_RS02895 - hypothetical protein, at 646,421 to 646,651
_RS02895
RR42_RS02900: RR42_RS02900 - LysR family transcriptional regulator, at 646,769 to 647,710
_RS02900
Position (kb)
646
647 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 645.533 kb on + strand at 645.534 kb on - strand at 645.534 kb on - strand at 645.560 kb on + strand at 645.561 kb on - strand at 645.561 kb on - strand at 645.611 kb on + strand at 645.611 kb on + strand at 645.667 kb on + strand at 645.668 kb on - strand at 645.668 kb on - strand at 645.741 kb on + strand, within RR42_RS02890 at 645.767 kb on + strand, within RR42_RS02890 at 645.767 kb on + strand, within RR42_RS02890 at 645.767 kb on + strand, within RR42_RS02890 at 645.768 kb on - strand, within RR42_RS02890 at 646.016 kb on + strand, within RR42_RS02890 at 646.016 kb on + strand, within RR42_RS02890 at 646.016 kb on + strand, within RR42_RS02890 at 646.016 kb on + strand, within RR42_RS02890 at 646.102 kb on + strand at 646.103 kb on - strand at 646.178 kb on + strand at 646.178 kb on + strand at 646.200 kb on - strand at 646.200 kb on - strand at 646.208 kb on + strand at 646.292 kb on - strand at 646.370 kb on - strand at 646.443 kb on + strand at 646.506 kb on + strand, within RR42_RS02895 at 646.506 kb on + strand, within RR42_RS02895 at 646.507 kb on - strand, within RR42_RS02895 at 646.507 kb on - strand, within RR42_RS02895 at 646.535 kb on + strand, within RR42_RS02895 at 646.536 kb on + strand, within RR42_RS02895 at 646.536 kb on + strand, within RR42_RS02895 at 646.536 kb on + strand, within RR42_RS02895 at 646.536 kb on + strand, within RR42_RS02895 at 646.536 kb on + strand, within RR42_RS02895 at 646.537 kb on - strand, within RR42_RS02895 at 646.537 kb on - strand, within RR42_RS02895 at 646.537 kb on - strand, within RR42_RS02895 at 646.537 kb on - strand, within RR42_RS02895 at 646.537 kb on - strand, within RR42_RS02895 at 646.537 kb on - strand, within RR42_RS02895 at 646.537 kb on - strand, within RR42_RS02895 at 646.656 kb on + strand at 646.657 kb on - strand at 646.657 kb on - strand at 646.741 kb on + strand at 646.770 kb on + strand at 646.902 kb on + strand, within RR42_RS02900 at 646.902 kb on + strand, within RR42_RS02900 at 646.902 kb on + strand, within RR42_RS02900 at 646.902 kb on + strand, within RR42_RS02900 at 646.902 kb on + strand, within RR42_RS02900 at 646.903 kb on - strand, within RR42_RS02900 at 646.903 kb on - strand, within RR42_RS02900 at 646.906 kb on - strand, within RR42_RS02900 at 646.965 kb on + strand, within RR42_RS02900 at 647.025 kb on - strand, within RR42_RS02900 at 647.046 kb on + strand, within RR42_RS02900 at 647.047 kb on - strand, within RR42_RS02900 at 647.250 kb on + strand, within RR42_RS02900 at 647.283 kb on + strand, within RR42_RS02900 at 647.283 kb on + strand, within RR42_RS02900 at 647.284 kb on - strand, within RR42_RS02900 at 647.489 kb on + strand, within RR42_RS02900 at 647.496 kb on + strand, within RR42_RS02900 at 647.496 kb on + strand, within RR42_RS02900 at 647.497 kb on - strand, within RR42_RS02900 at 647.502 kb on + strand, within RR42_RS02900 at 647.503 kb on - strand, within RR42_RS02900
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Zinc 0.1 mM remove 645,533 + +3.3 645,534 - +1.1 645,534 - -0.7 645,560 + +1.3 645,561 - -0.6 645,561 - +3.6 645,611 + -0.3 645,611 + -1.5 645,667 + +0.3 645,668 - -1.3 645,668 - -0.9 645,741 + RR42_RS02890 0.16 -2.3 645,767 + RR42_RS02890 0.22 -0.2 645,767 + RR42_RS02890 0.22 -1.8 645,767 + RR42_RS02890 0.22 +0.5 645,768 - RR42_RS02890 0.22 -0.2 646,016 + RR42_RS02890 0.81 -0.4 646,016 + RR42_RS02890 0.81 -0.0 646,016 + RR42_RS02890 0.81 -0.0 646,016 + RR42_RS02890 0.81 +0.3 646,102 + -0.3 646,103 - -0.8 646,178 + +0.2 646,178 + +0.6 646,200 - -0.6 646,200 - -0.8 646,208 + -0.0 646,292 - -1.6 646,370 - -0.5 646,443 + +0.4 646,506 + RR42_RS02895 0.37 -1.5 646,506 + RR42_RS02895 0.37 -2.2 646,507 - RR42_RS02895 0.37 -0.1 646,507 - RR42_RS02895 0.37 -0.5 646,535 + RR42_RS02895 0.49 +0.6 646,536 + RR42_RS02895 0.50 +1.6 646,536 + RR42_RS02895 0.50 -0.6 646,536 + RR42_RS02895 0.50 +0.4 646,536 + RR42_RS02895 0.50 -0.0 646,536 + RR42_RS02895 0.50 -2.0 646,537 - RR42_RS02895 0.50 -1.2 646,537 - RR42_RS02895 0.50 -0.3 646,537 - RR42_RS02895 0.50 +2.0 646,537 - RR42_RS02895 0.50 +1.8 646,537 - RR42_RS02895 0.50 -1.5 646,537 - RR42_RS02895 0.50 -1.6 646,537 - RR42_RS02895 0.50 +0.5 646,656 + +0.7 646,657 - +0.2 646,657 - +0.3 646,741 + -0.1 646,770 + +2.7 646,902 + RR42_RS02900 0.14 -0.2 646,902 + RR42_RS02900 0.14 -0.3 646,902 + RR42_RS02900 0.14 +0.5 646,902 + RR42_RS02900 0.14 +0.2 646,902 + RR42_RS02900 0.14 -2.9 646,903 - RR42_RS02900 0.14 +0.8 646,903 - RR42_RS02900 0.14 +1.0 646,906 - RR42_RS02900 0.15 -2.5 646,965 + RR42_RS02900 0.21 -0.3 647,025 - RR42_RS02900 0.27 -0.8 647,046 + RR42_RS02900 0.29 -3.1 647,047 - RR42_RS02900 0.30 -0.5 647,250 + RR42_RS02900 0.51 +0.3 647,283 + RR42_RS02900 0.55 +1.4 647,283 + RR42_RS02900 0.55 -0.0 647,284 - RR42_RS02900 0.55 +0.4 647,489 + RR42_RS02900 0.76 -0.3 647,496 + RR42_RS02900 0.77 +0.2 647,496 + RR42_RS02900 0.77 -0.6 647,497 - RR42_RS02900 0.77 -0.7 647,502 + RR42_RS02900 0.78 -0.8 647,503 - RR42_RS02900 0.78 -1.7
Or see this region's nucleotide sequence