Experiment: D-Gluconic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1882 and Pf6N2E2_1883 are separated by 101 nucleotides Pf6N2E2_1883 and Pf6N2E2_1884 are separated by 118 nucleotides Pf6N2E2_1884 and Pf6N2E2_1885 are separated by 666 nucleotides
Pf6N2E2_1882 - Thioredoxin reductase (EC 1.8.1.9), at 2,233,876 to 2,234,769
_1882
Pf6N2E2_1883 - Transcriptional regulator, TetR family, at 2,234,871 to 2,235,578
_1883
Pf6N2E2_1884 - oxidoreductase, short-chain dehydrogenase/reductase family, at 2,235,697 to 2,236,422
_1884
Pf6N2E2_1885 - Transcriptional regulator, AraC family, at 2,237,089 to 2,237,466
_1885
Position (kb)
2235
2236
2237 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2234.791 kb on + strand at 2234.791 kb on + strand at 2234.792 kb on - strand at 2234.843 kb on + strand at 2235.258 kb on + strand, within Pf6N2E2_1883 at 2235.276 kb on + strand, within Pf6N2E2_1883 at 2235.277 kb on - strand, within Pf6N2E2_1883 at 2235.278 kb on + strand, within Pf6N2E2_1883 at 2235.279 kb on - strand, within Pf6N2E2_1883 at 2235.279 kb on - strand, within Pf6N2E2_1883 at 2235.279 kb on - strand, within Pf6N2E2_1883 at 2235.279 kb on - strand, within Pf6N2E2_1883 at 2235.279 kb on - strand, within Pf6N2E2_1883 at 2235.445 kb on + strand, within Pf6N2E2_1883 at 2235.453 kb on + strand, within Pf6N2E2_1883 at 2235.454 kb on - strand, within Pf6N2E2_1883 at 2235.514 kb on - strand at 2235.514 kb on - strand at 2235.580 kb on + strand at 2235.580 kb on + strand at 2235.581 kb on - strand at 2235.581 kb on - strand at 2235.581 kb on - strand at 2235.605 kb on - strand at 2235.698 kb on + strand at 2235.698 kb on + strand at 2235.698 kb on + strand at 2235.698 kb on + strand at 2235.699 kb on - strand at 2235.699 kb on - strand at 2235.699 kb on - strand at 2235.721 kb on + strand at 2235.721 kb on + strand at 2235.877 kb on - strand, within Pf6N2E2_1884 at 2235.901 kb on - strand, within Pf6N2E2_1884 at 2235.909 kb on + strand, within Pf6N2E2_1884 at 2235.909 kb on + strand, within Pf6N2E2_1884 at 2235.909 kb on + strand, within Pf6N2E2_1884 at 2235.909 kb on + strand, within Pf6N2E2_1884 at 2235.910 kb on - strand, within Pf6N2E2_1884 at 2235.937 kb on - strand, within Pf6N2E2_1884 at 2235.969 kb on - strand, within Pf6N2E2_1884 at 2236.014 kb on + strand, within Pf6N2E2_1884 at 2236.014 kb on + strand, within Pf6N2E2_1884 at 2236.015 kb on - strand, within Pf6N2E2_1884 at 2236.393 kb on + strand at 2236.394 kb on - strand at 2236.464 kb on - strand at 2236.464 kb on - strand at 2236.464 kb on - strand at 2236.485 kb on - strand at 2236.496 kb on - strand at 2236.496 kb on - strand at 2236.501 kb on + strand at 2236.501 kb on + strand at 2236.677 kb on + strand at 2236.677 kb on + strand at 2236.768 kb on + strand at 2236.769 kb on - strand at 2236.783 kb on + strand at 2236.784 kb on - strand at 2236.784 kb on - strand at 2236.784 kb on - strand at 2236.784 kb on - strand at 2236.784 kb on - strand at 2236.787 kb on + strand at 2236.788 kb on - strand at 2236.788 kb on - strand at 2236.815 kb on - strand at 2236.880 kb on + strand at 2236.881 kb on - strand at 2236.883 kb on + strand at 2236.884 kb on - strand at 2236.884 kb on - strand at 2236.934 kb on + strand at 2236.941 kb on - strand at 2236.941 kb on - strand at 2236.950 kb on - strand at 2236.950 kb on - strand at 2237.042 kb on + strand at 2237.060 kb on - strand at 2237.062 kb on - strand at 2237.062 kb on - strand at 2237.132 kb on + strand, within Pf6N2E2_1885 at 2237.132 kb on + strand, within Pf6N2E2_1885 at 2237.151 kb on - strand, within Pf6N2E2_1885 at 2237.270 kb on - strand, within Pf6N2E2_1885
Per-strain Table
Position Strand Gene LocusTag Fraction D-Gluconic Acid (C) remove 2,234,791 + -1.2 2,234,791 + -2.4 2,234,792 - -0.4 2,234,843 + -0.3 2,235,258 + Pf6N2E2_1883 0.55 +0.3 2,235,276 + Pf6N2E2_1883 0.57 +0.2 2,235,277 - Pf6N2E2_1883 0.57 +0.6 2,235,278 + Pf6N2E2_1883 0.57 +2.8 2,235,279 - Pf6N2E2_1883 0.58 +0.4 2,235,279 - Pf6N2E2_1883 0.58 +0.9 2,235,279 - Pf6N2E2_1883 0.58 +0.7 2,235,279 - Pf6N2E2_1883 0.58 -0.1 2,235,279 - Pf6N2E2_1883 0.58 -0.2 2,235,445 + Pf6N2E2_1883 0.81 +0.8 2,235,453 + Pf6N2E2_1883 0.82 +0.5 2,235,454 - Pf6N2E2_1883 0.82 -0.4 2,235,514 - -0.4 2,235,514 - -1.5 2,235,580 + +0.3 2,235,580 + +0.1 2,235,581 - +0.6 2,235,581 - -2.4 2,235,581 - +0.0 2,235,605 - +0.4 2,235,698 + +0.8 2,235,698 + +0.1 2,235,698 + +0.8 2,235,698 + -0.2 2,235,699 - -0.3 2,235,699 - +0.4 2,235,699 - -2.8 2,235,721 + +0.6 2,235,721 + +0.5 2,235,877 - Pf6N2E2_1884 0.25 -0.3 2,235,901 - Pf6N2E2_1884 0.28 +0.9 2,235,909 + Pf6N2E2_1884 0.29 -1.2 2,235,909 + Pf6N2E2_1884 0.29 -1.0 2,235,909 + Pf6N2E2_1884 0.29 -1.0 2,235,909 + Pf6N2E2_1884 0.29 -0.6 2,235,910 - Pf6N2E2_1884 0.29 -1.4 2,235,937 - Pf6N2E2_1884 0.33 -1.8 2,235,969 - Pf6N2E2_1884 0.37 +0.2 2,236,014 + Pf6N2E2_1884 0.44 -1.4 2,236,014 + Pf6N2E2_1884 0.44 -0.7 2,236,015 - Pf6N2E2_1884 0.44 -2.4 2,236,393 + +0.4 2,236,394 - -0.4 2,236,464 - +0.7 2,236,464 - +1.4 2,236,464 - -1.4 2,236,485 - +0.9 2,236,496 - +0.8 2,236,496 - +2.6 2,236,501 + -0.2 2,236,501 + +1.2 2,236,677 + +0.8 2,236,677 + +1.0 2,236,768 + +0.5 2,236,769 - -0.8 2,236,783 + -0.0 2,236,784 - -1.4 2,236,784 - +0.9 2,236,784 - -1.0 2,236,784 - +0.4 2,236,784 - -1.3 2,236,787 + +0.4 2,236,788 - -0.3 2,236,788 - -0.9 2,236,815 - +0.2 2,236,880 + -0.6 2,236,881 - +0.5 2,236,883 + +0.3 2,236,884 - -0.9 2,236,884 - +1.6 2,236,934 + -0.5 2,236,941 - +0.6 2,236,941 - +0.6 2,236,950 - +0.6 2,236,950 - -0.9 2,237,042 + -0.2 2,237,060 - -0.2 2,237,062 - +0.7 2,237,062 - -0.7 2,237,132 + Pf6N2E2_1885 0.11 +0.0 2,237,132 + Pf6N2E2_1885 0.11 +1.1 2,237,151 - Pf6N2E2_1885 0.16 +0.6 2,237,270 - Pf6N2E2_1885 0.48 +0.9
Or see this region's nucleotide sequence