Experiment: R2A with sodium fluoride 25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS15195 and CA265_RS15200 are separated by 5 nucleotides CA265_RS15200 and CA265_RS15205 are separated by 130 nucleotides CA265_RS15205 and CA265_RS15210 are separated by 52 nucleotides CA265_RS15210 and CA265_RS15215 are separated by 134 nucleotides
CA265_RS15195: CA265_RS15195 - hypothetical protein, at 3,824,535 to 3,825,191
_RS15195
CA265_RS15200: CA265_RS15200 - hypothetical protein, at 3,825,197 to 3,825,637
_RS15200
CA265_RS15205: CA265_RS15205 - aspartate aminotransferase family protein, at 3,825,768 to 3,826,904
_RS15205
CA265_RS15210: CA265_RS15210 - DNA-binding response regulator, at 3,826,957 to 3,827,634
_RS15210
CA265_RS15215: CA265_RS15215 - two-component sensor histidine kinase, at 3,827,769 to 3,829,073
_RS15215
Position (kb)
3825
3826
3827 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3824.777 kb on - strand, within CA265_RS15195 at 3824.879 kb on + strand, within CA265_RS15195 at 3824.913 kb on - strand, within CA265_RS15195 at 3824.913 kb on - strand, within CA265_RS15195 at 3824.913 kb on - strand, within CA265_RS15195 at 3824.916 kb on + strand, within CA265_RS15195 at 3825.031 kb on + strand, within CA265_RS15195 at 3825.032 kb on - strand, within CA265_RS15195 at 3825.060 kb on - strand, within CA265_RS15195 at 3825.071 kb on - strand, within CA265_RS15195 at 3825.084 kb on - strand, within CA265_RS15195 at 3825.100 kb on + strand, within CA265_RS15195 at 3825.101 kb on - strand, within CA265_RS15195 at 3825.108 kb on - strand, within CA265_RS15195 at 3825.139 kb on - strand at 3825.169 kb on + strand at 3825.169 kb on + strand at 3825.222 kb on + strand at 3825.318 kb on + strand, within CA265_RS15200 at 3825.319 kb on - strand, within CA265_RS15200 at 3825.319 kb on - strand, within CA265_RS15200 at 3825.353 kb on + strand, within CA265_RS15200 at 3825.361 kb on + strand, within CA265_RS15200 at 3825.604 kb on - strand at 3825.659 kb on + strand at 3825.660 kb on - strand at 3825.660 kb on - strand at 3825.676 kb on - strand at 3825.768 kb on - strand at 3825.781 kb on + strand at 3825.781 kb on + strand at 3825.798 kb on + strand at 3825.805 kb on - strand at 3825.857 kb on + strand at 3825.857 kb on + strand at 3825.915 kb on + strand, within CA265_RS15205 at 3825.917 kb on + strand, within CA265_RS15205 at 3825.918 kb on - strand, within CA265_RS15205 at 3825.942 kb on + strand, within CA265_RS15205 at 3825.943 kb on - strand, within CA265_RS15205 at 3826.092 kb on - strand, within CA265_RS15205 at 3826.110 kb on + strand, within CA265_RS15205 at 3826.118 kb on + strand, within CA265_RS15205 at 3826.122 kb on - strand, within CA265_RS15205 at 3826.193 kb on - strand, within CA265_RS15205 at 3826.234 kb on + strand, within CA265_RS15205 at 3826.271 kb on - strand, within CA265_RS15205 at 3826.289 kb on - strand, within CA265_RS15205 at 3826.308 kb on + strand, within CA265_RS15205 at 3826.309 kb on - strand, within CA265_RS15205 at 3826.309 kb on - strand, within CA265_RS15205 at 3826.311 kb on - strand, within CA265_RS15205 at 3826.426 kb on + strand, within CA265_RS15205 at 3826.521 kb on - strand, within CA265_RS15205 at 3826.591 kb on + strand, within CA265_RS15205 at 3826.591 kb on + strand, within CA265_RS15205 at 3826.592 kb on - strand, within CA265_RS15205 at 3826.771 kb on + strand, within CA265_RS15205 at 3826.772 kb on - strand, within CA265_RS15205 at 3826.830 kb on - strand at 3826.879 kb on + strand at 3826.964 kb on + strand at 3826.964 kb on + strand at 3826.965 kb on - strand at 3827.020 kb on - strand at 3827.031 kb on + strand, within CA265_RS15210 at 3827.032 kb on - strand, within CA265_RS15210 at 3827.076 kb on - strand, within CA265_RS15210 at 3827.082 kb on - strand, within CA265_RS15210 at 3827.153 kb on + strand, within CA265_RS15210 at 3827.223 kb on + strand, within CA265_RS15210 at 3827.251 kb on + strand, within CA265_RS15210 at 3827.292 kb on + strand, within CA265_RS15210 at 3827.349 kb on + strand, within CA265_RS15210 at 3827.508 kb on - strand, within CA265_RS15210 at 3827.625 kb on + strand at 3827.687 kb on + strand at 3827.705 kb on - strand at 3827.746 kb on - strand at 3827.797 kb on - strand at 3827.811 kb on - strand at 3827.858 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium fluoride 25 mM remove 3,824,777 - CA265_RS15195 0.37 +0.2 3,824,879 + CA265_RS15195 0.52 -3.0 3,824,913 - CA265_RS15195 0.58 +0.3 3,824,913 - CA265_RS15195 0.58 -0.5 3,824,913 - CA265_RS15195 0.58 +0.2 3,824,916 + CA265_RS15195 0.58 -0.7 3,825,031 + CA265_RS15195 0.75 -1.0 3,825,032 - CA265_RS15195 0.76 +0.5 3,825,060 - CA265_RS15195 0.80 -0.1 3,825,071 - CA265_RS15195 0.82 -0.6 3,825,084 - CA265_RS15195 0.84 -1.7 3,825,100 + CA265_RS15195 0.86 +0.7 3,825,101 - CA265_RS15195 0.86 +0.8 3,825,108 - CA265_RS15195 0.87 -0.3 3,825,139 - -0.0 3,825,169 + -0.2 3,825,169 + +0.5 3,825,222 + +1.1 3,825,318 + CA265_RS15200 0.27 -0.5 3,825,319 - CA265_RS15200 0.28 +1.1 3,825,319 - CA265_RS15200 0.28 -0.8 3,825,353 + CA265_RS15200 0.35 +0.2 3,825,361 + CA265_RS15200 0.37 +0.1 3,825,604 - +0.6 3,825,659 + +0.4 3,825,660 - +0.6 3,825,660 - -0.0 3,825,676 - +0.7 3,825,768 - -3.6 3,825,781 + -1.2 3,825,781 + -0.4 3,825,798 + -0.2 3,825,805 - -1.7 3,825,857 + -2.3 3,825,857 + -0.4 3,825,915 + CA265_RS15205 0.13 -1.6 3,825,917 + CA265_RS15205 0.13 -2.3 3,825,918 - CA265_RS15205 0.13 -1.7 3,825,942 + CA265_RS15205 0.15 -2.8 3,825,943 - CA265_RS15205 0.15 -0.7 3,826,092 - CA265_RS15205 0.28 -2.3 3,826,110 + CA265_RS15205 0.30 -3.1 3,826,118 + CA265_RS15205 0.31 -1.7 3,826,122 - CA265_RS15205 0.31 -4.7 3,826,193 - CA265_RS15205 0.37 -3.0 3,826,234 + CA265_RS15205 0.41 -1.4 3,826,271 - CA265_RS15205 0.44 -0.8 3,826,289 - CA265_RS15205 0.46 -3.9 3,826,308 + CA265_RS15205 0.47 -2.7 3,826,309 - CA265_RS15205 0.48 -3.1 3,826,309 - CA265_RS15205 0.48 -3.2 3,826,311 - CA265_RS15205 0.48 -2.4 3,826,426 + CA265_RS15205 0.58 -1.0 3,826,521 - CA265_RS15205 0.66 +0.6 3,826,591 + CA265_RS15205 0.72 -3.0 3,826,591 + CA265_RS15205 0.72 -3.8 3,826,592 - CA265_RS15205 0.72 -4.0 3,826,771 + CA265_RS15205 0.88 -2.4 3,826,772 - CA265_RS15205 0.88 -3.3 3,826,830 - -2.4 3,826,879 + -0.4 3,826,964 + +0.3 3,826,964 + -0.5 3,826,965 - -0.8 3,827,020 - -0.6 3,827,031 + CA265_RS15210 0.11 -1.0 3,827,032 - CA265_RS15210 0.11 -0.6 3,827,076 - CA265_RS15210 0.18 -1.1 3,827,082 - CA265_RS15210 0.18 -1.9 3,827,153 + CA265_RS15210 0.29 -1.8 3,827,223 + CA265_RS15210 0.39 -1.1 3,827,251 + CA265_RS15210 0.43 -0.8 3,827,292 + CA265_RS15210 0.49 -1.1 3,827,349 + CA265_RS15210 0.58 -1.4 3,827,508 - CA265_RS15210 0.81 -2.0 3,827,625 + +0.7 3,827,687 + -0.6 3,827,705 - +0.4 3,827,746 - -0.1 3,827,797 - -0.8 3,827,811 - -0.7 3,827,858 - -0.1
Or see this region's nucleotide sequence