Experiment: R2A with Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS36525 and RR42_RS36530 are separated by 36 nucleotides RR42_RS36530 and RR42_RS36535 are separated by 277 nucleotides RR42_RS36535 and RR42_RS36540 are separated by 84 nucleotides RR42_RS36540 and RR42_RS36545 are separated by 17 nucleotides RR42_RS36545 and RR42_RS36550 are separated by 4 nucleotides
RR42_RS36525: RR42_RS36525 - universal stress protein UspA, at 3,651,961 to 3,652,395
_RS36525
RR42_RS36530: RR42_RS36530 - hypothetical protein, at 3,652,432 to 3,652,650
_RS36530
RR42_RS36535: RR42_RS36535 - hypothetical protein, at 3,652,928 to 3,653,176
_RS36535
RR42_RS36540: RR42_RS36540 - CoA-transferase, at 3,653,261 to 3,654,484
_RS36540
RR42_RS36545: RR42_RS36545 - 3-alpha,7-alpha, 12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase, at 3,654,502 to 3,655,365
_RS36545
RR42_RS36550: RR42_RS36550 - 3-hydroxyacyl-CoA dehydrogenase, at 3,655,370 to 3,656,284
_RS36550
Position (kb)
3653
3654
3655 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3652.289 kb on + strand, within RR42_RS36525 at 3652.289 kb on + strand, within RR42_RS36525 at 3652.289 kb on + strand, within RR42_RS36525 at 3652.289 kb on + strand, within RR42_RS36525 at 3652.290 kb on - strand, within RR42_RS36525 at 3652.290 kb on - strand, within RR42_RS36525 at 3652.290 kb on - strand, within RR42_RS36525 at 3652.463 kb on + strand, within RR42_RS36530 at 3652.464 kb on - strand, within RR42_RS36530 at 3652.624 kb on + strand, within RR42_RS36530 at 3652.625 kb on - strand, within RR42_RS36530 at 3652.728 kb on + strand at 3652.728 kb on + strand at 3652.729 kb on - strand at 3652.859 kb on + strand at 3652.866 kb on + strand at 3652.893 kb on - strand at 3652.895 kb on - strand at 3653.244 kb on + strand at 3653.443 kb on + strand, within RR42_RS36540 at 3653.464 kb on - strand, within RR42_RS36540 at 3653.496 kb on + strand, within RR42_RS36540 at 3653.497 kb on - strand, within RR42_RS36540 at 3653.573 kb on - strand, within RR42_RS36540 at 3653.679 kb on + strand, within RR42_RS36540 at 3653.680 kb on - strand, within RR42_RS36540 at 3653.721 kb on + strand, within RR42_RS36540 at 3653.721 kb on + strand, within RR42_RS36540 at 3653.722 kb on - strand, within RR42_RS36540 at 3653.722 kb on - strand, within RR42_RS36540 at 3653.722 kb on - strand, within RR42_RS36540 at 3653.748 kb on + strand, within RR42_RS36540 at 3653.759 kb on - strand, within RR42_RS36540 at 3653.819 kb on - strand, within RR42_RS36540 at 3653.838 kb on - strand, within RR42_RS36540 at 3653.928 kb on + strand, within RR42_RS36540 at 3653.929 kb on - strand, within RR42_RS36540 at 3654.048 kb on + strand, within RR42_RS36540 at 3654.102 kb on + strand, within RR42_RS36540 at 3654.103 kb on - strand, within RR42_RS36540 at 3654.103 kb on - strand, within RR42_RS36540 at 3654.103 kb on - strand, within RR42_RS36540 at 3654.138 kb on + strand, within RR42_RS36540 at 3654.139 kb on - strand, within RR42_RS36540 at 3654.139 kb on - strand, within RR42_RS36540 at 3654.174 kb on + strand, within RR42_RS36540 at 3654.175 kb on - strand, within RR42_RS36540 at 3654.175 kb on - strand, within RR42_RS36540 at 3654.175 kb on - strand, within RR42_RS36540 at 3654.285 kb on + strand, within RR42_RS36540 at 3654.288 kb on + strand, within RR42_RS36540 at 3654.288 kb on + strand, within RR42_RS36540 at 3654.288 kb on + strand, within RR42_RS36540 at 3654.289 kb on - strand, within RR42_RS36540 at 3654.461 kb on + strand at 3654.508 kb on - strand at 3654.508 kb on - strand at 3654.691 kb on - strand, within RR42_RS36545 at 3654.720 kb on - strand, within RR42_RS36545 at 3655.223 kb on + strand, within RR42_RS36545 at 3655.223 kb on + strand, within RR42_RS36545 at 3655.308 kb on - strand at 3655.308 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml remove 3,652,289 + RR42_RS36525 0.75 -0.2 3,652,289 + RR42_RS36525 0.75 -0.7 3,652,289 + RR42_RS36525 0.75 -0.1 3,652,289 + RR42_RS36525 0.75 +0.8 3,652,290 - RR42_RS36525 0.76 -0.3 3,652,290 - RR42_RS36525 0.76 -0.3 3,652,290 - RR42_RS36525 0.76 +1.7 3,652,463 + RR42_RS36530 0.14 -1.1 3,652,464 - RR42_RS36530 0.15 +0.9 3,652,624 + RR42_RS36530 0.88 -0.5 3,652,625 - RR42_RS36530 0.88 -0.7 3,652,728 + +0.6 3,652,728 + -1.1 3,652,729 - -0.1 3,652,859 + -0.3 3,652,866 + -1.0 3,652,893 - -0.5 3,652,895 - +0.1 3,653,244 + +3.6 3,653,443 + RR42_RS36540 0.15 +0.5 3,653,464 - RR42_RS36540 0.17 +0.3 3,653,496 + RR42_RS36540 0.19 +1.1 3,653,497 - RR42_RS36540 0.19 +0.9 3,653,573 - RR42_RS36540 0.25 -1.5 3,653,679 + RR42_RS36540 0.34 +0.9 3,653,680 - RR42_RS36540 0.34 -0.8 3,653,721 + RR42_RS36540 0.38 -1.3 3,653,721 + RR42_RS36540 0.38 -1.1 3,653,722 - RR42_RS36540 0.38 +1.1 3,653,722 - RR42_RS36540 0.38 +1.4 3,653,722 - RR42_RS36540 0.38 -0.7 3,653,748 + RR42_RS36540 0.40 +0.1 3,653,759 - RR42_RS36540 0.41 -0.1 3,653,819 - RR42_RS36540 0.46 -0.6 3,653,838 - RR42_RS36540 0.47 +0.6 3,653,928 + RR42_RS36540 0.54 +2.6 3,653,929 - RR42_RS36540 0.55 +0.8 3,654,048 + RR42_RS36540 0.64 +0.6 3,654,102 + RR42_RS36540 0.69 -2.4 3,654,103 - RR42_RS36540 0.69 +0.3 3,654,103 - RR42_RS36540 0.69 +1.6 3,654,103 - RR42_RS36540 0.69 -0.2 3,654,138 + RR42_RS36540 0.72 +0.2 3,654,139 - RR42_RS36540 0.72 +0.5 3,654,139 - RR42_RS36540 0.72 +0.3 3,654,174 + RR42_RS36540 0.75 +0.1 3,654,175 - RR42_RS36540 0.75 -0.4 3,654,175 - RR42_RS36540 0.75 -0.5 3,654,175 - RR42_RS36540 0.75 +0.5 3,654,285 + RR42_RS36540 0.84 +0.9 3,654,288 + RR42_RS36540 0.84 -0.4 3,654,288 + RR42_RS36540 0.84 +1.2 3,654,288 + RR42_RS36540 0.84 -0.0 3,654,289 - RR42_RS36540 0.84 -0.5 3,654,461 + +1.1 3,654,508 - -0.8 3,654,508 - -0.5 3,654,691 - RR42_RS36545 0.22 -1.1 3,654,720 - RR42_RS36545 0.25 -0.7 3,655,223 + RR42_RS36545 0.83 +0.6 3,655,223 + RR42_RS36545 0.83 -0.5 3,655,308 - +0.4 3,655,308 - -0.3
Or see this region's nucleotide sequence