Experiment: R2A with Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32380 and RR42_RS32385 overlap by 4 nucleotides RR42_RS32385 and RR42_RS32390 are separated by 62 nucleotides RR42_RS32390 and RR42_RS32395 are separated by 219 nucleotides
RR42_RS32380: RR42_RS32380 - CoA-transferase, at 2,659,418 to 2,660,617
_RS32380
RR42_RS32385: RR42_RS32385 - citrate synthase, at 2,660,614 to 2,661,480
_RS32385
RR42_RS32390: RR42_RS32390 - citrate synthase, at 2,661,543 to 2,662,772
_RS32390
RR42_RS32395: RR42_RS32395 - IclR family transcriptional regulator, at 2,662,992 to 2,663,720
_RS32395
Position (kb)
2661
2662
2663 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2660.614 kb on - strand at 2660.625 kb on + strand at 2661.001 kb on + strand, within RR42_RS32385 at 2661.002 kb on - strand, within RR42_RS32385 at 2661.002 kb on - strand, within RR42_RS32385 at 2661.074 kb on - strand, within RR42_RS32385 at 2661.293 kb on - strand, within RR42_RS32385 at 2661.424 kb on + strand at 2661.424 kb on + strand at 2661.424 kb on + strand at 2661.424 kb on + strand at 2661.425 kb on - strand at 2661.425 kb on - strand at 2661.425 kb on - strand at 2661.425 kb on - strand at 2661.436 kb on + strand at 2661.655 kb on + strand at 2661.656 kb on - strand at 2661.656 kb on - strand at 2661.874 kb on + strand, within RR42_RS32390 at 2662.225 kb on + strand, within RR42_RS32390 at 2662.225 kb on + strand, within RR42_RS32390 at 2662.226 kb on - strand, within RR42_RS32390 at 2662.226 kb on - strand, within RR42_RS32390 at 2662.345 kb on + strand, within RR42_RS32390 at 2662.345 kb on + strand, within RR42_RS32390 at 2662.345 kb on + strand, within RR42_RS32390 at 2662.345 kb on + strand, within RR42_RS32390 at 2662.345 kb on + strand, within RR42_RS32390 at 2662.514 kb on - strand, within RR42_RS32390 at 2662.514 kb on - strand, within RR42_RS32390 at 2662.516 kb on + strand, within RR42_RS32390 at 2662.516 kb on + strand, within RR42_RS32390 at 2662.517 kb on - strand, within RR42_RS32390 at 2662.557 kb on - strand, within RR42_RS32390 at 2662.585 kb on + strand, within RR42_RS32390 at 2662.586 kb on - strand, within RR42_RS32390 at 2662.586 kb on - strand, within RR42_RS32390 at 2662.726 kb on + strand at 2662.727 kb on - strand at 2662.727 kb on - strand at 2662.727 kb on - strand at 2662.764 kb on + strand at 2662.764 kb on + strand at 2662.765 kb on - strand at 2662.789 kb on + strand at 2663.149 kb on - strand, within RR42_RS32395 at 2663.205 kb on + strand, within RR42_RS32395 at 2663.335 kb on + strand, within RR42_RS32395 at 2663.371 kb on + strand, within RR42_RS32395 at 2663.371 kb on + strand, within RR42_RS32395 at 2663.372 kb on - strand, within RR42_RS32395 at 2663.372 kb on - strand, within RR42_RS32395 at 2663.452 kb on + strand, within RR42_RS32395 at 2663.452 kb on + strand, within RR42_RS32395 at 2663.652 kb on + strand at 2663.711 kb on + strand at 2663.711 kb on + strand at 2663.712 kb on - strand at 2663.712 kb on - strand at 2663.712 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml remove 2,660,614 - +0.7 2,660,625 + +0.7 2,661,001 + RR42_RS32385 0.45 +1.3 2,661,002 - RR42_RS32385 0.45 +1.4 2,661,002 - RR42_RS32385 0.45 -0.5 2,661,074 - RR42_RS32385 0.53 +1.4 2,661,293 - RR42_RS32385 0.78 -0.7 2,661,424 + -1.2 2,661,424 + -1.1 2,661,424 + +0.3 2,661,424 + +1.0 2,661,425 - -1.3 2,661,425 - +0.4 2,661,425 - +0.6 2,661,425 - +1.2 2,661,436 + -2.4 2,661,655 + +0.3 2,661,656 - -1.3 2,661,656 - -0.1 2,661,874 + RR42_RS32390 0.27 -0.4 2,662,225 + RR42_RS32390 0.55 -0.3 2,662,225 + RR42_RS32390 0.55 -0.4 2,662,226 - RR42_RS32390 0.56 +0.1 2,662,226 - RR42_RS32390 0.56 +0.1 2,662,345 + RR42_RS32390 0.65 -1.0 2,662,345 + RR42_RS32390 0.65 +0.0 2,662,345 + RR42_RS32390 0.65 +0.7 2,662,345 + RR42_RS32390 0.65 -0.1 2,662,345 + RR42_RS32390 0.65 +0.4 2,662,514 - RR42_RS32390 0.79 -1.5 2,662,514 - RR42_RS32390 0.79 -1.0 2,662,516 + RR42_RS32390 0.79 -0.5 2,662,516 + RR42_RS32390 0.79 +0.8 2,662,517 - RR42_RS32390 0.79 -0.7 2,662,557 - RR42_RS32390 0.82 -0.2 2,662,585 + RR42_RS32390 0.85 -0.8 2,662,586 - RR42_RS32390 0.85 +0.2 2,662,586 - RR42_RS32390 0.85 -0.0 2,662,726 + -2.7 2,662,727 - +0.7 2,662,727 - +1.6 2,662,727 - -0.5 2,662,764 + -1.0 2,662,764 + +0.4 2,662,765 - -1.0 2,662,789 + -0.1 2,663,149 - RR42_RS32395 0.22 -0.3 2,663,205 + RR42_RS32395 0.29 +0.5 2,663,335 + RR42_RS32395 0.47 +1.4 2,663,371 + RR42_RS32395 0.52 +0.7 2,663,371 + RR42_RS32395 0.52 +0.5 2,663,372 - RR42_RS32395 0.52 +0.7 2,663,372 - RR42_RS32395 0.52 +2.2 2,663,452 + RR42_RS32395 0.63 +0.3 2,663,452 + RR42_RS32395 0.63 +0.7 2,663,652 + +0.0 2,663,711 + -1.5 2,663,711 + +0.5 2,663,712 - -1.0 2,663,712 - +0.6 2,663,712 - +0.4
Or see this region's nucleotide sequence