Experiment: R2A with Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS28855 and RR42_RS28860 are separated by 85 nucleotides RR42_RS28860 and RR42_RS28865 are separated by 57 nucleotides RR42_RS28865 and RR42_RS28870 are separated by 145 nucleotides
RR42_RS28855: RR42_RS28855 - amino acid deaminase, at 1,827,180 to 1,828,448
_RS28855
RR42_RS28860: RR42_RS28860 - 2-dehydro-3-deoxygluconokinase (EC 2.7.1.45) (from data) , at 1,828,534 to 1,829,469
_RS28860
RR42_RS28865: RR42_RS28865 - 2-dehydro-3-deoxyphosphogluconate aldolase, at 1,829,527 to 1,830,171
_RS28865
RR42_RS28870: RR42_RS28870 - phospholipase D, at 1,830,317 to 1,831,903
_RS28870
Position (kb)
1828
1829
1830 Strain fitness (log2 ratio)
-2
-1
0
1 at 1827.557 kb on + strand, within RR42_RS28855 at 1827.605 kb on + strand, within RR42_RS28855 at 1827.648 kb on + strand, within RR42_RS28855 at 1827.936 kb on + strand, within RR42_RS28855 at 1828.125 kb on + strand, within RR42_RS28855 at 1828.296 kb on + strand, within RR42_RS28855 at 1828.370 kb on + strand at 1828.370 kb on + strand at 1828.371 kb on - strand at 1828.371 kb on - strand at 1828.512 kb on + strand at 1828.512 kb on + strand at 1828.512 kb on + strand at 1828.512 kb on + strand at 1828.512 kb on + strand at 1828.512 kb on + strand at 1828.512 kb on + strand at 1828.513 kb on - strand at 1828.514 kb on + strand at 1828.678 kb on + strand, within RR42_RS28860 at 1828.678 kb on + strand, within RR42_RS28860 at 1828.678 kb on + strand, within RR42_RS28860 at 1828.678 kb on + strand, within RR42_RS28860 at 1828.680 kb on + strand, within RR42_RS28860 at 1828.680 kb on + strand, within RR42_RS28860 at 1828.792 kb on + strand, within RR42_RS28860 at 1828.792 kb on + strand, within RR42_RS28860 at 1828.792 kb on + strand, within RR42_RS28860 at 1828.792 kb on + strand, within RR42_RS28860 at 1828.792 kb on + strand, within RR42_RS28860 at 1828.792 kb on + strand, within RR42_RS28860 at 1828.832 kb on - strand, within RR42_RS28860 at 1828.861 kb on + strand, within RR42_RS28860 at 1828.906 kb on + strand, within RR42_RS28860 at 1829.159 kb on - strand, within RR42_RS28860 at 1829.215 kb on + strand, within RR42_RS28860 at 1829.215 kb on + strand, within RR42_RS28860 at 1829.216 kb on - strand, within RR42_RS28860 at 1830.221 kb on + strand at 1830.221 kb on + strand at 1830.221 kb on + strand at 1830.222 kb on - strand at 1830.222 kb on - strand at 1830.222 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml remove 1,827,557 + RR42_RS28855 0.30 -1.8 1,827,605 + RR42_RS28855 0.33 -0.9 1,827,648 + RR42_RS28855 0.37 -0.6 1,827,936 + RR42_RS28855 0.60 -0.6 1,828,125 + RR42_RS28855 0.74 +0.5 1,828,296 + RR42_RS28855 0.88 +0.5 1,828,370 + +0.5 1,828,370 + -0.7 1,828,371 - +1.1 1,828,371 - -1.3 1,828,512 + -0.2 1,828,512 + +0.1 1,828,512 + -1.1 1,828,512 + -1.3 1,828,512 + -0.1 1,828,512 + +0.9 1,828,512 + +1.7 1,828,513 - +0.7 1,828,514 + -0.8 1,828,678 + RR42_RS28860 0.15 +0.4 1,828,678 + RR42_RS28860 0.15 +0.7 1,828,678 + RR42_RS28860 0.15 +1.2 1,828,678 + RR42_RS28860 0.15 +0.7 1,828,680 + RR42_RS28860 0.16 +1.1 1,828,680 + RR42_RS28860 0.16 -1.8 1,828,792 + RR42_RS28860 0.28 -0.2 1,828,792 + RR42_RS28860 0.28 -0.9 1,828,792 + RR42_RS28860 0.28 -2.0 1,828,792 + RR42_RS28860 0.28 +0.5 1,828,792 + RR42_RS28860 0.28 -0.1 1,828,792 + RR42_RS28860 0.28 -1.2 1,828,832 - RR42_RS28860 0.32 -0.9 1,828,861 + RR42_RS28860 0.35 +0.5 1,828,906 + RR42_RS28860 0.40 +0.3 1,829,159 - RR42_RS28860 0.67 -1.3 1,829,215 + RR42_RS28860 0.73 -0.5 1,829,215 + RR42_RS28860 0.73 -1.8 1,829,216 - RR42_RS28860 0.73 -1.9 1,830,221 + +0.1 1,830,221 + +0.0 1,830,221 + -0.5 1,830,222 - -0.5 1,830,222 - -0.1 1,830,222 - -0.6
Or see this region's nucleotide sequence