Experiment: R2A with Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS28410 and RR42_RS28415 are separated by 5 nucleotides RR42_RS28415 and RR42_RS28420 are separated by 2 nucleotides RR42_RS28420 and RR42_RS28425 are separated by 15 nucleotides RR42_RS28425 and RR42_RS28430 are separated by 15 nucleotides
RR42_RS28410: RR42_RS28410 - dimethylmenaquinone methyltransferase, at 1,734,504 to 1,735,166
_RS28410
RR42_RS28415: RR42_RS28415 - diguanylate cyclase, at 1,735,172 to 1,735,834
_RS28415
RR42_RS28420: RR42_RS28420 - 4-hydroxy-4-methyl-2-oxoglutarate aldolase, at 1,735,837 to 1,736,499
_RS28420
RR42_RS28425: RR42_RS28425 - 3-hydroxybutyryl-CoA dehydratase, at 1,736,515 to 1,737,285
_RS28425
RR42_RS28430: RR42_RS28430 - phenylacetic acid degradation protein, at 1,737,301 to 1,737,723
_RS28430
Position (kb) 1735
1736
1737 Strain fitness (log2 ratio) -2
-1
0
1 at 1734.890 kb on + strand, within RR42_RS28410 at 1734.911 kb on + strand, within RR42_RS28410 at 1734.990 kb on + strand, within RR42_RS28410 at 1735.143 kb on + strand at 1735.164 kb on + strand at 1735.165 kb on - strand at 1735.320 kb on - strand, within RR42_RS28415 at 1735.331 kb on + strand, within RR42_RS28415 at 1735.332 kb on - strand, within RR42_RS28415 at 1735.370 kb on + strand, within RR42_RS28415 at 1735.371 kb on - strand, within RR42_RS28415 at 1735.411 kb on + strand, within RR42_RS28415 at 1735.412 kb on - strand, within RR42_RS28415 at 1735.533 kb on + strand, within RR42_RS28415 at 1735.533 kb on + strand, within RR42_RS28415 at 1735.608 kb on + strand, within RR42_RS28415 at 1735.609 kb on - strand, within RR42_RS28415 at 1736.048 kb on + strand, within RR42_RS28420 at 1736.048 kb on + strand, within RR42_RS28420 at 1736.049 kb on - strand, within RR42_RS28420 at 1736.049 kb on - strand, within RR42_RS28420 at 1736.049 kb on - strand, within RR42_RS28420 at 1736.278 kb on + strand, within RR42_RS28420 at 1736.299 kb on - strand, within RR42_RS28420 at 1736.369 kb on + strand, within RR42_RS28420 at 1736.370 kb on - strand, within RR42_RS28420 at 1736.405 kb on + strand, within RR42_RS28420 at 1736.405 kb on + strand, within RR42_RS28420 at 1736.485 kb on + strand at 1736.578 kb on - strand at 1736.929 kb on + strand, within RR42_RS28425 at 1736.950 kb on + strand, within RR42_RS28425 at 1736.951 kb on - strand, within RR42_RS28425 at 1737.317 kb on - strand at 1737.342 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml remove 1,734,890 + RR42_RS28410 0.58 -0.8 1,734,911 + RR42_RS28410 0.61 -0.4 1,734,990 + RR42_RS28410 0.73 -1.3 1,735,143 + +0.2 1,735,164 + -0.3 1,735,165 - +0.1 1,735,320 - RR42_RS28415 0.22 -0.5 1,735,331 + RR42_RS28415 0.24 -0.2 1,735,332 - RR42_RS28415 0.24 -0.8 1,735,370 + RR42_RS28415 0.30 +0.1 1,735,371 - RR42_RS28415 0.30 +0.1 1,735,411 + RR42_RS28415 0.36 +0.2 1,735,412 - RR42_RS28415 0.36 +0.2 1,735,533 + RR42_RS28415 0.54 -0.8 1,735,533 + RR42_RS28415 0.54 +0.4 1,735,608 + RR42_RS28415 0.66 +0.7 1,735,609 - RR42_RS28415 0.66 -1.4 1,736,048 + RR42_RS28420 0.32 -0.3 1,736,048 + RR42_RS28420 0.32 +0.9 1,736,049 - RR42_RS28420 0.32 -0.9 1,736,049 - RR42_RS28420 0.32 -0.4 1,736,049 - RR42_RS28420 0.32 -0.9 1,736,278 + RR42_RS28420 0.67 +1.0 1,736,299 - RR42_RS28420 0.70 -0.7 1,736,369 + RR42_RS28420 0.80 -1.5 1,736,370 - RR42_RS28420 0.80 +0.2 1,736,405 + RR42_RS28420 0.86 -0.6 1,736,405 + RR42_RS28420 0.86 -0.6 1,736,485 + -0.6 1,736,578 - +0.2 1,736,929 + RR42_RS28425 0.54 +0.4 1,736,950 + RR42_RS28425 0.56 +0.6 1,736,951 - RR42_RS28425 0.57 -1.8 1,737,317 - -2.7 1,737,342 - -2.3
Or see this region's nucleotide sequence