Experiment: R2A with Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23965 and RR42_RS23970 are separated by 172 nucleotides RR42_RS23970 and RR42_RS23975 are separated by 71 nucleotides RR42_RS23975 and RR42_RS23980 are separated by 130 nucleotides
RR42_RS23965: RR42_RS23965 - hypothetical protein, at 701,867 to 704,476
_RS23965
RR42_RS23970: RR42_RS23970 - hypothetical protein, at 704,649 to 704,867
_RS23970
RR42_RS23975: RR42_RS23975 - hypothetical protein, at 704,939 to 705,865
_RS23975
RR42_RS23980: RR42_RS23980 - hypothetical protein, at 705,996 to 706,577
_RS23980
Position (kb) 704
705
706 Strain fitness (log2 ratio) -2
-1
0
1
2 at 704.103 kb on - strand, within RR42_RS23965 at 704.184 kb on + strand, within RR42_RS23965 at 704.185 kb on - strand, within RR42_RS23965 at 704.185 kb on - strand, within RR42_RS23965 at 704.251 kb on + strand at 704.252 kb on - strand at 704.401 kb on + strand at 704.536 kb on - strand at 704.722 kb on + strand, within RR42_RS23970 at 704.723 kb on - strand, within RR42_RS23970 at 704.778 kb on - strand, within RR42_RS23970 at 704.813 kb on - strand, within RR42_RS23970 at 704.869 kb on - strand at 704.904 kb on + strand at 704.905 kb on + strand at 704.905 kb on + strand at 704.936 kb on + strand at 704.936 kb on + strand at 704.937 kb on - strand at 704.937 kb on - strand at 704.937 kb on - strand at 704.937 kb on - strand at 704.937 kb on - strand at 704.941 kb on - strand at 704.941 kb on - strand at 704.973 kb on - strand at 705.249 kb on - strand, within RR42_RS23975 at 705.266 kb on - strand, within RR42_RS23975 at 705.290 kb on + strand, within RR42_RS23975 at 705.290 kb on + strand, within RR42_RS23975 at 705.290 kb on + strand, within RR42_RS23975 at 705.294 kb on + strand, within RR42_RS23975 at 705.299 kb on - strand, within RR42_RS23975 at 705.343 kb on - strand, within RR42_RS23975 at 705.343 kb on - strand, within RR42_RS23975 at 705.486 kb on + strand, within RR42_RS23975 at 705.583 kb on - strand, within RR42_RS23975 at 705.583 kb on - strand, within RR42_RS23975 at 705.596 kb on - strand, within RR42_RS23975 at 705.804 kb on + strand at 705.804 kb on + strand at 705.827 kb on + strand at 705.827 kb on + strand at 705.912 kb on - strand at 705.962 kb on + strand at 705.963 kb on - strand at 706.192 kb on + strand, within RR42_RS23980 at 706.263 kb on + strand, within RR42_RS23980 at 706.264 kb on - strand, within RR42_RS23980 at 706.264 kb on - strand, within RR42_RS23980 at 706.264 kb on - strand, within RR42_RS23980 at 706.386 kb on + strand, within RR42_RS23980 at 706.386 kb on + strand, within RR42_RS23980 at 706.387 kb on - strand, within RR42_RS23980 at 706.387 kb on - strand, within RR42_RS23980 at 706.517 kb on - strand, within RR42_RS23980 at 706.528 kb on - strand at 706.577 kb on + strand at 706.577 kb on + strand at 706.578 kb on - strand at 706.578 kb on - strand at 706.578 kb on - strand at 706.578 kb on - strand at 706.783 kb on - strand at 706.823 kb on + strand at 706.824 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml remove 704,103 - RR42_RS23965 0.86 +1.4 704,184 + RR42_RS23965 0.89 +0.0 704,185 - RR42_RS23965 0.89 +1.7 704,185 - RR42_RS23965 0.89 +2.0 704,251 + +0.5 704,252 - -0.5 704,401 + -0.7 704,536 - +1.2 704,722 + RR42_RS23970 0.33 +1.1 704,723 - RR42_RS23970 0.34 +0.7 704,778 - RR42_RS23970 0.59 -0.8 704,813 - RR42_RS23970 0.75 +0.6 704,869 - -1.3 704,904 + +0.8 704,905 + -0.3 704,905 + -0.5 704,936 + +0.0 704,936 + +0.0 704,937 - +1.2 704,937 - +2.0 704,937 - +2.6 704,937 - -1.0 704,937 - +0.0 704,941 - +0.7 704,941 - +0.3 704,973 - +0.4 705,249 - RR42_RS23975 0.33 +0.9 705,266 - RR42_RS23975 0.35 +0.7 705,290 + RR42_RS23975 0.38 -0.5 705,290 + RR42_RS23975 0.38 -1.3 705,290 + RR42_RS23975 0.38 +0.9 705,294 + RR42_RS23975 0.38 +1.0 705,299 - RR42_RS23975 0.39 -0.2 705,343 - RR42_RS23975 0.44 -0.6 705,343 - RR42_RS23975 0.44 -0.9 705,486 + RR42_RS23975 0.59 -0.0 705,583 - RR42_RS23975 0.69 +1.0 705,583 - RR42_RS23975 0.69 +0.4 705,596 - RR42_RS23975 0.71 +0.4 705,804 + +0.8 705,804 + -1.6 705,827 + -2.0 705,827 + +0.7 705,912 - -1.0 705,962 + -1.9 705,963 - -0.4 706,192 + RR42_RS23980 0.34 +0.9 706,263 + RR42_RS23980 0.46 +0.7 706,264 - RR42_RS23980 0.46 +0.8 706,264 - RR42_RS23980 0.46 -0.3 706,264 - RR42_RS23980 0.46 +0.5 706,386 + RR42_RS23980 0.67 +1.1 706,386 + RR42_RS23980 0.67 -0.6 706,387 - RR42_RS23980 0.67 -0.8 706,387 - RR42_RS23980 0.67 -0.4 706,517 - RR42_RS23980 0.90 -0.3 706,528 - -0.1 706,577 + -0.8 706,577 + -0.3 706,578 - +0.6 706,578 - -0.6 706,578 - -0.8 706,578 - +0.1 706,783 - -0.8 706,823 + -2.1 706,824 - +0.5
Or see this region's nucleotide sequence