Experiment: R2A with Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23090 and RR42_RS23095 are separated by 92 nucleotides RR42_RS23095 and RR42_RS23100 are separated by 89 nucleotides RR42_RS23100 and RR42_RS23105 are separated by 135 nucleotides RR42_RS23105 and RR42_RS23110 are separated by 193 nucleotides
RR42_RS23090: RR42_RS23090 - 2,5-dioxovalerate dehydrogenase, at 514,711 to 516,312
_RS23090
RR42_RS23095: RR42_RS23095 - Zn-dependent hydrolase, at 516,405 to 517,169
_RS23095
RR42_RS23100: RR42_RS23100 - AraC family transcriptional regulator, at 517,259 to 518,224
_RS23100
RR42_RS23105: RR42_RS23105 - hypothetical protein, at 518,360 to 518,818
_RS23105
RR42_RS23110: RR42_RS23110 - isochorismatase, at 519,012 to 519,542
_RS23110
Position (kb) 517
518
519 Strain fitness (log2 ratio) -2
-1
0
1
2 at 516.304 kb on - strand at 516.428 kb on + strand at 516.428 kb on + strand at 516.675 kb on + strand, within RR42_RS23095 at 516.695 kb on - strand, within RR42_RS23095 at 516.695 kb on - strand, within RR42_RS23095 at 516.715 kb on - strand, within RR42_RS23095 at 516.715 kb on - strand, within RR42_RS23095 at 516.872 kb on - strand, within RR42_RS23095 at 517.163 kb on + strand at 517.163 kb on + strand at 517.163 kb on + strand at 517.163 kb on + strand at 517.164 kb on - strand at 517.164 kb on - strand at 517.164 kb on - strand at 517.515 kb on + strand, within RR42_RS23100 at 517.584 kb on + strand, within RR42_RS23100 at 517.592 kb on + strand, within RR42_RS23100 at 517.592 kb on + strand, within RR42_RS23100 at 517.592 kb on + strand, within RR42_RS23100 at 517.593 kb on - strand, within RR42_RS23100 at 517.593 kb on - strand, within RR42_RS23100 at 517.593 kb on - strand, within RR42_RS23100 at 517.688 kb on + strand, within RR42_RS23100 at 517.688 kb on + strand, within RR42_RS23100 at 517.689 kb on - strand, within RR42_RS23100 at 517.738 kb on + strand, within RR42_RS23100 at 518.052 kb on - strand, within RR42_RS23100 at 518.052 kb on - strand, within RR42_RS23100 at 518.052 kb on - strand, within RR42_RS23100 at 518.092 kb on + strand, within RR42_RS23100 at 518.118 kb on + strand, within RR42_RS23100 at 518.118 kb on + strand, within RR42_RS23100 at 518.119 kb on - strand, within RR42_RS23100 at 518.119 kb on - strand, within RR42_RS23100 at 518.119 kb on - strand, within RR42_RS23100 at 518.155 kb on + strand at 518.155 kb on + strand at 518.202 kb on - strand at 518.250 kb on + strand at 518.251 kb on - strand at 518.419 kb on - strand, within RR42_RS23105 at 518.419 kb on - strand, within RR42_RS23105 at 518.608 kb on + strand, within RR42_RS23105 at 518.608 kb on + strand, within RR42_RS23105 at 518.665 kb on - strand, within RR42_RS23105 at 518.665 kb on - strand, within RR42_RS23105 at 518.671 kb on - strand, within RR42_RS23105 at 518.671 kb on - strand, within RR42_RS23105 at 518.671 kb on - strand, within RR42_RS23105 at 518.704 kb on - strand, within RR42_RS23105 at 518.918 kb on + strand at 518.918 kb on + strand at 518.918 kb on + strand at 518.982 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml remove 516,304 - -0.6 516,428 + -1.1 516,428 + -1.8 516,675 + RR42_RS23095 0.35 -1.3 516,695 - RR42_RS23095 0.38 +0.3 516,695 - RR42_RS23095 0.38 +0.8 516,715 - RR42_RS23095 0.41 -0.6 516,715 - RR42_RS23095 0.41 +0.2 516,872 - RR42_RS23095 0.61 -0.3 517,163 + +0.5 517,163 + -1.5 517,163 + +0.0 517,163 + -0.0 517,164 - -2.0 517,164 - +0.1 517,164 - +1.3 517,515 + RR42_RS23100 0.27 +0.3 517,584 + RR42_RS23100 0.34 -0.0 517,592 + RR42_RS23100 0.34 -2.5 517,592 + RR42_RS23100 0.34 +0.2 517,592 + RR42_RS23100 0.34 -1.0 517,593 - RR42_RS23100 0.35 +0.6 517,593 - RR42_RS23100 0.35 +0.8 517,593 - RR42_RS23100 0.35 -0.7 517,688 + RR42_RS23100 0.44 -0.2 517,688 + RR42_RS23100 0.44 +0.3 517,689 - RR42_RS23100 0.45 -0.6 517,738 + RR42_RS23100 0.50 +1.0 518,052 - RR42_RS23100 0.82 -0.8 518,052 - RR42_RS23100 0.82 -0.6 518,052 - RR42_RS23100 0.82 -2.0 518,092 + RR42_RS23100 0.86 -0.4 518,118 + RR42_RS23100 0.89 +0.0 518,118 + RR42_RS23100 0.89 +2.3 518,119 - RR42_RS23100 0.89 -0.8 518,119 - RR42_RS23100 0.89 +1.0 518,119 - RR42_RS23100 0.89 -0.9 518,155 + -0.2 518,155 + -0.4 518,202 - -0.1 518,250 + -0.7 518,251 - -0.9 518,419 - RR42_RS23105 0.13 -1.2 518,419 - RR42_RS23105 0.13 +0.9 518,608 + RR42_RS23105 0.54 -0.3 518,608 + RR42_RS23105 0.54 +0.5 518,665 - RR42_RS23105 0.66 +0.1 518,665 - RR42_RS23105 0.66 -2.0 518,671 - RR42_RS23105 0.68 +0.3 518,671 - RR42_RS23105 0.68 +0.7 518,671 - RR42_RS23105 0.68 -0.2 518,704 - RR42_RS23105 0.75 -0.7 518,918 + +0.0 518,918 + +1.3 518,918 + -0.4 518,982 - +0.0
Or see this region's nucleotide sequence