Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS22000

Experiment: R2A with Cisplatin 0.001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS21995 and RR42_RS22000 are separated by 1 nucleotidesRR42_RS22000 and RR42_RS22005 are separated by 201 nucleotides RR42_RS21995: RR42_RS21995 - glycosyl transferase family 1, at 265,574 to 267,175 _RS21995 RR42_RS22000: RR42_RS22000 - histidine kinase, at 267,177 to 268,547 _RS22000 RR42_RS22005: RR42_RS22005 - transcriptional regulator, at 268,749 to 269,432 _RS22005 Position (kb) 267 268 269Strain fitness (log2 ratio) -2 -1 0 1 2 3at 266.231 kb on + strand, within RR42_RS21995at 266.231 kb on + strand, within RR42_RS21995at 266.232 kb on - strand, within RR42_RS21995at 266.232 kb on - strand, within RR42_RS21995at 266.232 kb on - strand, within RR42_RS21995at 266.232 kb on - strand, within RR42_RS21995at 266.461 kb on + strand, within RR42_RS21995at 266.462 kb on - strand, within RR42_RS21995at 266.582 kb on - strand, within RR42_RS21995at 266.663 kb on + strand, within RR42_RS21995at 266.663 kb on + strand, within RR42_RS21995at 266.806 kb on - strand, within RR42_RS21995at 267.035 kb on + strandat 267.074 kb on + strandat 267.074 kb on + strandat 267.075 kb on - strandat 267.253 kb on - strandat 267.378 kb on + strand, within RR42_RS22000at 267.379 kb on - strand, within RR42_RS22000at 267.438 kb on - strand, within RR42_RS22000at 267.487 kb on - strand, within RR42_RS22000at 267.658 kb on - strand, within RR42_RS22000at 267.693 kb on - strand, within RR42_RS22000at 267.693 kb on - strand, within RR42_RS22000at 267.693 kb on - strand, within RR42_RS22000at 267.730 kb on - strand, within RR42_RS22000at 267.813 kb on + strand, within RR42_RS22000at 267.814 kb on - strand, within RR42_RS22000at 267.814 kb on - strand, within RR42_RS22000at 267.859 kb on - strand, within RR42_RS22000at 267.894 kb on + strand, within RR42_RS22000at 267.894 kb on + strand, within RR42_RS22000at 267.895 kb on - strand, within RR42_RS22000at 267.895 kb on - strand, within RR42_RS22000at 267.895 kb on - strand, within RR42_RS22000at 267.895 kb on - strand, within RR42_RS22000at 267.987 kb on + strand, within RR42_RS22000at 267.987 kb on + strand, within RR42_RS22000at 267.988 kb on - strand, within RR42_RS22000at 268.089 kb on + strand, within RR42_RS22000at 268.089 kb on + strand, within RR42_RS22000at 268.089 kb on + strand, within RR42_RS22000at 268.124 kb on + strand, within RR42_RS22000at 268.133 kb on + strand, within RR42_RS22000at 268.274 kb on - strand, within RR42_RS22000at 268.278 kb on + strand, within RR42_RS22000at 268.279 kb on - strand, within RR42_RS22000at 268.279 kb on - strand, within RR42_RS22000at 268.279 kb on - strand, within RR42_RS22000at 268.335 kb on + strand, within RR42_RS22000at 268.335 kb on + strand, within RR42_RS22000at 268.336 kb on - strand, within RR42_RS22000at 268.336 kb on - strand, within RR42_RS22000at 268.341 kb on + strand, within RR42_RS22000at 268.440 kb on + strandat 268.491 kb on + strandat 268.533 kb on + strandat 268.533 kb on + strandat 268.586 kb on - strandat 268.627 kb on + strandat 268.628 kb on - strandat 268.645 kb on + strandat 268.744 kb on + strandat 268.744 kb on + strandat 268.745 kb on - strandat 268.745 kb on - strandat 268.745 kb on - strandat 268.748 kb on + strandat 268.749 kb on - strandat 269.043 kb on + strand, within RR42_RS22005at 269.044 kb on - strand, within RR42_RS22005at 269.044 kb on - strand, within RR42_RS22005at 269.044 kb on - strand, within RR42_RS22005at 269.087 kb on - strand, within RR42_RS22005at 269.154 kb on + strand, within RR42_RS22005at 269.155 kb on - strand, within RR42_RS22005at 269.155 kb on - strand, within RR42_RS22005at 269.155 kb on - strand, within RR42_RS22005at 269.155 kb on - strand, within RR42_RS22005at 269.333 kb on + strand, within RR42_RS22005at 269.339 kb on + strand, within RR42_RS22005at 269.340 kb on - strand, within RR42_RS22005at 269.340 kb on - strand, within RR42_RS22005at 269.340 kb on - strand, within RR42_RS22005at 269.340 kb on - strand, within RR42_RS22005at 269.404 kb on - strandat 269.490 kb on - strandat 269.517 kb on + strandat 269.518 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml
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266,231 + RR42_RS21995 0.41 +0.0
266,231 + RR42_RS21995 0.41 +0.0
266,232 - RR42_RS21995 0.41 -1.7
266,232 - RR42_RS21995 0.41 +0.1
266,232 - RR42_RS21995 0.41 -1.2
266,232 - RR42_RS21995 0.41 +1.2
266,461 + RR42_RS21995 0.55 -0.6
266,462 - RR42_RS21995 0.55 -0.2
266,582 - RR42_RS21995 0.63 +0.7
266,663 + RR42_RS21995 0.68 -0.4
266,663 + RR42_RS21995 0.68 +1.4
266,806 - RR42_RS21995 0.77 -0.8
267,035 + -0.1
267,074 + -0.1
267,074 + -0.1
267,075 - +0.3
267,253 - +0.0
267,378 + RR42_RS22000 0.15 -0.6
267,379 - RR42_RS22000 0.15 -0.8
267,438 - RR42_RS22000 0.19 -1.8
267,487 - RR42_RS22000 0.23 +1.8
267,658 - RR42_RS22000 0.35 +0.4
267,693 - RR42_RS22000 0.38 -1.0
267,693 - RR42_RS22000 0.38 -0.1
267,693 - RR42_RS22000 0.38 -1.6
267,730 - RR42_RS22000 0.40 -0.6
267,813 + RR42_RS22000 0.46 +0.3
267,814 - RR42_RS22000 0.46 -1.6
267,814 - RR42_RS22000 0.46 +1.4
267,859 - RR42_RS22000 0.50 -1.5
267,894 + RR42_RS22000 0.52 +1.8
267,894 + RR42_RS22000 0.52 +0.5
267,895 - RR42_RS22000 0.52 +0.4
267,895 - RR42_RS22000 0.52 -1.7
267,895 - RR42_RS22000 0.52 -1.8
267,895 - RR42_RS22000 0.52 +1.6
267,987 + RR42_RS22000 0.59 -0.4
267,987 + RR42_RS22000 0.59 -1.0
267,988 - RR42_RS22000 0.59 -2.2
268,089 + RR42_RS22000 0.67 -0.6
268,089 + RR42_RS22000 0.67 -1.9
268,089 + RR42_RS22000 0.67 -0.6
268,124 + RR42_RS22000 0.69 -1.2
268,133 + RR42_RS22000 0.70 -0.0
268,274 - RR42_RS22000 0.80 -1.6
268,278 + RR42_RS22000 0.80 -0.4
268,279 - RR42_RS22000 0.80 -0.1
268,279 - RR42_RS22000 0.80 +1.4
268,279 - RR42_RS22000 0.80 -0.2
268,335 + RR42_RS22000 0.84 +1.6
268,335 + RR42_RS22000 0.84 +0.2
268,336 - RR42_RS22000 0.85 -0.3
268,336 - RR42_RS22000 0.85 +0.4
268,341 + RR42_RS22000 0.85 -0.6
268,440 + -0.9
268,491 + -1.2
268,533 + -0.2
268,533 + +0.5
268,586 - +0.0
268,627 + +0.1
268,628 - -0.6
268,645 + +0.3
268,744 + +0.0
268,744 + +0.1
268,745 - -0.4
268,745 - -1.1
268,745 - +0.8
268,748 + +0.3
268,749 - +1.2
269,043 + RR42_RS22005 0.43 +1.2
269,044 - RR42_RS22005 0.43 -0.4
269,044 - RR42_RS22005 0.43 -1.0
269,044 - RR42_RS22005 0.43 -1.0
269,087 - RR42_RS22005 0.49 -0.6
269,154 + RR42_RS22005 0.59 +1.7
269,155 - RR42_RS22005 0.59 -1.2
269,155 - RR42_RS22005 0.59 -0.8
269,155 - RR42_RS22005 0.59 -1.1
269,155 - RR42_RS22005 0.59 -0.8
269,333 + RR42_RS22005 0.85 +0.4
269,339 + RR42_RS22005 0.86 +2.0
269,340 - RR42_RS22005 0.86 -1.2
269,340 - RR42_RS22005 0.86 +0.4
269,340 - RR42_RS22005 0.86 -0.3
269,340 - RR42_RS22005 0.86 +0.3
269,404 - +3.0
269,490 - +0.4
269,517 + +2.1
269,518 - -1.1

Or see this region's nucleotide sequence