Experiment: R2A with Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21645 and RR42_RS21650 are separated by 43 nucleotides RR42_RS21650 and RR42_RS21655 are separated by 162 nucleotides RR42_RS21655 and RR42_RS21660 are separated by 7 nucleotides RR42_RS21660 and RR42_RS21665 are separated by 106 nucleotides
RR42_RS21645: RR42_RS21645 - N-carbamoylsarcosine amidase, at 185,451 to 186,092
_RS21645
RR42_RS21650: RR42_RS21650 - TetR family transcriptional regulator, at 186,136 to 186,873
_RS21650
RR42_RS21655: RR42_RS21655 - aldehyde dehydrogenase, at 187,036 to 189,300
_RS21655
RR42_RS21660: RR42_RS21660 - MarR family transcriptional regulator, at 189,308 to 189,820
_RS21660
RR42_RS21665: RR42_RS21665 - cobalt transporter, at 189,927 to 190,907
_RS21665
Position (kb) 187
188
189
190 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 186.090 kb on + strand at 186.243 kb on + strand, within RR42_RS21650 at 186.318 kb on + strand, within RR42_RS21650 at 186.318 kb on + strand, within RR42_RS21650 at 186.319 kb on - strand, within RR42_RS21650 at 186.336 kb on - strand, within RR42_RS21650 at 186.366 kb on - strand, within RR42_RS21650 at 186.366 kb on - strand, within RR42_RS21650 at 186.449 kb on + strand, within RR42_RS21650 at 186.449 kb on + strand, within RR42_RS21650 at 186.548 kb on + strand, within RR42_RS21650 at 186.549 kb on - strand, within RR42_RS21650 at 186.551 kb on + strand, within RR42_RS21650 at 186.551 kb on + strand, within RR42_RS21650 at 186.552 kb on - strand, within RR42_RS21650 at 186.644 kb on + strand, within RR42_RS21650 at 186.644 kb on + strand, within RR42_RS21650 at 186.644 kb on + strand, within RR42_RS21650 at 186.692 kb on + strand, within RR42_RS21650 at 186.693 kb on - strand, within RR42_RS21650 at 186.693 kb on - strand, within RR42_RS21650 at 186.695 kb on + strand, within RR42_RS21650 at 186.698 kb on + strand, within RR42_RS21650 at 186.698 kb on + strand, within RR42_RS21650 at 186.698 kb on + strand, within RR42_RS21650 at 186.699 kb on - strand, within RR42_RS21650 at 186.699 kb on - strand, within RR42_RS21650 at 186.699 kb on - strand, within RR42_RS21650 at 186.699 kb on - strand, within RR42_RS21650 at 186.699 kb on - strand, within RR42_RS21650 at 186.873 kb on + strand at 186.873 kb on + strand at 186.873 kb on + strand at 186.874 kb on - strand at 186.883 kb on - strand at 186.883 kb on - strand at 186.886 kb on + strand at 186.886 kb on + strand at 186.887 kb on - strand at 186.910 kb on - strand at 186.910 kb on - strand at 186.911 kb on + strand at 187.531 kb on + strand, within RR42_RS21655 at 187.641 kb on + strand, within RR42_RS21655 at 187.729 kb on + strand, within RR42_RS21655 at 187.730 kb on - strand, within RR42_RS21655 at 188.066 kb on - strand, within RR42_RS21655 at 188.111 kb on - strand, within RR42_RS21655 at 188.239 kb on + strand, within RR42_RS21655 at 188.536 kb on + strand, within RR42_RS21655 at 188.542 kb on + strand, within RR42_RS21655 at 188.831 kb on - strand, within RR42_RS21655 at 188.841 kb on + strand, within RR42_RS21655 at 188.841 kb on + strand, within RR42_RS21655 at 189.309 kb on + strand at 189.966 kb on + strand at 189.968 kb on + strand at 189.969 kb on - strand at 189.969 kb on - strand at 189.969 kb on - strand at 189.969 kb on - strand at 190.146 kb on + strand, within RR42_RS21665 at 190.146 kb on + strand, within RR42_RS21665
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml remove 186,090 + -1.3 186,243 + RR42_RS21650 0.14 -1.4 186,318 + RR42_RS21650 0.25 -2.8 186,318 + RR42_RS21650 0.25 -0.7 186,319 - RR42_RS21650 0.25 -0.2 186,336 - RR42_RS21650 0.27 +0.7 186,366 - RR42_RS21650 0.31 +0.7 186,366 - RR42_RS21650 0.31 -0.8 186,449 + RR42_RS21650 0.42 +1.4 186,449 + RR42_RS21650 0.42 -0.5 186,548 + RR42_RS21650 0.56 -0.4 186,549 - RR42_RS21650 0.56 +0.6 186,551 + RR42_RS21650 0.56 -0.7 186,551 + RR42_RS21650 0.56 -0.6 186,552 - RR42_RS21650 0.56 +0.8 186,644 + RR42_RS21650 0.69 -1.2 186,644 + RR42_RS21650 0.69 +0.5 186,644 + RR42_RS21650 0.69 -0.4 186,692 + RR42_RS21650 0.75 -0.4 186,693 - RR42_RS21650 0.75 +2.5 186,693 - RR42_RS21650 0.75 -0.1 186,695 + RR42_RS21650 0.76 +0.9 186,698 + RR42_RS21650 0.76 +0.1 186,698 + RR42_RS21650 0.76 +1.7 186,698 + RR42_RS21650 0.76 -0.7 186,699 - RR42_RS21650 0.76 -0.0 186,699 - RR42_RS21650 0.76 +0.6 186,699 - RR42_RS21650 0.76 -0.9 186,699 - RR42_RS21650 0.76 +1.0 186,699 - RR42_RS21650 0.76 +0.9 186,873 + +1.0 186,873 + -2.4 186,873 + -1.0 186,874 - +1.1 186,883 - -0.9 186,883 - +0.3 186,886 + +0.5 186,886 + +1.4 186,887 - +0.2 186,910 - -0.5 186,910 - +0.3 186,911 + -0.1 187,531 + RR42_RS21655 0.22 -1.6 187,641 + RR42_RS21655 0.27 -1.8 187,729 + RR42_RS21655 0.31 +1.0 187,730 - RR42_RS21655 0.31 -1.0 188,066 - RR42_RS21655 0.45 +2.0 188,111 - RR42_RS21655 0.47 -1.3 188,239 + RR42_RS21655 0.53 -0.9 188,536 + RR42_RS21655 0.66 -1.7 188,542 + RR42_RS21655 0.66 -0.2 188,831 - RR42_RS21655 0.79 -0.8 188,841 + RR42_RS21655 0.80 -0.3 188,841 + RR42_RS21655 0.80 -0.7 189,309 + -2.1 189,966 + +1.0 189,968 + -0.4 189,969 - -1.1 189,969 - -2.2 189,969 - -0.1 189,969 - -0.8 190,146 + RR42_RS21665 0.22 -0.8 190,146 + RR42_RS21665 0.22 -1.8
Or see this region's nucleotide sequence