Experiment: R2A with Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21370 and RR42_RS21375 are separated by 116 nucleotides RR42_RS21375 and RR42_RS21380 are separated by 40 nucleotides RR42_RS21380 and RR42_RS21385 are separated by 18 nucleotides
RR42_RS21370: RR42_RS21370 - 4-aminobutyrate aminotransferase, at 123,938 to 125,200
_RS21370
RR42_RS21375: RR42_RS21375 - succinate-semialdehyde dehydrogenase, at 125,317 to 126,783
_RS21375
RR42_RS21380: RR42_RS21380 - cupin, at 126,824 to 127,141
_RS21380
RR42_RS21385: RR42_RS21385 - acetylpolyamine aminohydrolase, at 127,160 to 128,233
_RS21385
Position (kb) 125
126
127 Strain fitness (log2 ratio) -2
-1
0
1 at 124.390 kb on - strand, within RR42_RS21370 at 124.398 kb on - strand, within RR42_RS21370 at 125.105 kb on + strand at 125.284 kb on - strand at 125.554 kb on + strand, within RR42_RS21375 at 125.555 kb on - strand, within RR42_RS21375 at 126.212 kb on - strand, within RR42_RS21375 at 126.229 kb on + strand, within RR42_RS21375 at 126.229 kb on + strand, within RR42_RS21375 at 126.740 kb on - strand at 126.755 kb on - strand at 126.781 kb on + strand at 126.799 kb on + strand at 126.938 kb on + strand, within RR42_RS21380 at 126.938 kb on + strand, within RR42_RS21380 at 127.451 kb on + strand, within RR42_RS21385 at 127.454 kb on + strand, within RR42_RS21385 at 127.454 kb on + strand, within RR42_RS21385 at 127.455 kb on - strand, within RR42_RS21385 at 127.455 kb on - strand, within RR42_RS21385 at 127.455 kb on - strand, within RR42_RS21385 at 127.526 kb on + strand, within RR42_RS21385 at 127.527 kb on - strand, within RR42_RS21385 at 127.527 kb on - strand, within RR42_RS21385 at 127.650 kb on - strand, within RR42_RS21385
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml remove 124,390 - RR42_RS21370 0.36 -0.7 124,398 - RR42_RS21370 0.36 -0.1 125,105 + -0.7 125,284 - -1.1 125,554 + RR42_RS21375 0.16 +0.8 125,555 - RR42_RS21375 0.16 +0.3 126,212 - RR42_RS21375 0.61 -0.1 126,229 + RR42_RS21375 0.62 -0.2 126,229 + RR42_RS21375 0.62 -0.3 126,740 - +0.6 126,755 - -1.2 126,781 + +0.2 126,799 + +0.7 126,938 + RR42_RS21380 0.36 -0.6 126,938 + RR42_RS21380 0.36 +0.7 127,451 + RR42_RS21385 0.27 +0.5 127,454 + RR42_RS21385 0.27 +0.4 127,454 + RR42_RS21385 0.27 +0.7 127,455 - RR42_RS21385 0.27 -0.2 127,455 - RR42_RS21385 0.27 -0.1 127,455 - RR42_RS21385 0.27 -0.8 127,526 + RR42_RS21385 0.34 +1.0 127,527 - RR42_RS21385 0.34 -1.0 127,527 - RR42_RS21385 0.34 -2.3 127,650 - RR42_RS21385 0.46 -0.5
Or see this region's nucleotide sequence