Experiment: R2A with Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21185 and RR42_RS21190 are separated by 58 nucleotides RR42_RS21190 and RR42_RS21195 are separated by 67 nucleotides RR42_RS21195 and RR42_RS21200 are separated by 52 nucleotides
RR42_RS21185: RR42_RS21185 - AMP nucleosidase, at 77,087 to 78,583
_RS21185
RR42_RS21190: RR42_RS21190 - LysR family transcriptional regulator, at 78,642 to 79,085
_RS21190
RR42_RS21195: RR42_RS21195 - phosphohydrolase, at 79,153 to 79,848
_RS21195
RR42_RS21200: RR42_RS21200 - membrane protein, at 79,901 to 80,545
_RS21200
Position (kb) 79
80 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 78.296 kb on + strand, within RR42_RS21185 at 78.396 kb on + strand, within RR42_RS21185 at 78.396 kb on + strand, within RR42_RS21185 at 78.396 kb on + strand, within RR42_RS21185 at 78.396 kb on + strand, within RR42_RS21185 at 78.397 kb on - strand, within RR42_RS21185 at 78.397 kb on - strand, within RR42_RS21185 at 78.459 kb on - strand at 78.481 kb on + strand at 78.481 kb on + strand at 78.482 kb on - strand at 78.482 kb on - strand at 78.482 kb on - strand at 78.482 kb on - strand at 78.482 kb on - strand at 78.582 kb on - strand at 78.734 kb on + strand, within RR42_RS21190 at 78.735 kb on - strand, within RR42_RS21190 at 78.735 kb on - strand, within RR42_RS21190 at 78.735 kb on - strand, within RR42_RS21190 at 78.784 kb on + strand, within RR42_RS21190 at 78.784 kb on + strand, within RR42_RS21190 at 78.785 kb on - strand, within RR42_RS21190 at 78.785 kb on - strand, within RR42_RS21190 at 78.823 kb on + strand, within RR42_RS21190 at 78.824 kb on - strand, within RR42_RS21190 at 78.971 kb on - strand, within RR42_RS21190 at 79.096 kb on + strand at 79.097 kb on - strand at 79.097 kb on - strand at 79.097 kb on - strand at 79.097 kb on - strand at 79.106 kb on - strand at 79.159 kb on - strand at 79.310 kb on + strand, within RR42_RS21195 at 79.364 kb on + strand, within RR42_RS21195 at 79.439 kb on + strand, within RR42_RS21195 at 79.439 kb on + strand, within RR42_RS21195 at 79.439 kb on + strand, within RR42_RS21195 at 79.439 kb on + strand, within RR42_RS21195 at 79.439 kb on + strand, within RR42_RS21195 at 79.439 kb on + strand, within RR42_RS21195 at 79.440 kb on - strand, within RR42_RS21195 at 79.440 kb on - strand, within RR42_RS21195 at 79.724 kb on + strand, within RR42_RS21195 at 79.724 kb on + strand, within RR42_RS21195 at 79.795 kb on + strand at 79.796 kb on - strand at 79.796 kb on - strand at 79.890 kb on - strand at 79.890 kb on - strand at 80.043 kb on + strand, within RR42_RS21200 at 80.044 kb on - strand, within RR42_RS21200 at 80.084 kb on - strand, within RR42_RS21200 at 80.154 kb on + strand, within RR42_RS21200 at 80.154 kb on + strand, within RR42_RS21200 at 80.155 kb on - strand, within RR42_RS21200 at 80.155 kb on - strand, within RR42_RS21200 at 80.155 kb on - strand, within RR42_RS21200 at 80.188 kb on - strand, within RR42_RS21200 at 80.247 kb on + strand, within RR42_RS21200 at 80.311 kb on - strand, within RR42_RS21200 at 80.410 kb on - strand, within RR42_RS21200 at 80.529 kb on + strand at 80.530 kb on - strand at 80.530 kb on - strand at 80.727 kb on - strand at 80.742 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml remove 78,296 + RR42_RS21185 0.81 -2.7 78,396 + RR42_RS21185 0.87 -0.7 78,396 + RR42_RS21185 0.87 +0.8 78,396 + RR42_RS21185 0.87 -0.9 78,396 + RR42_RS21185 0.87 -1.5 78,397 - RR42_RS21185 0.88 -0.5 78,397 - RR42_RS21185 0.88 -0.4 78,459 - -1.2 78,481 + +0.1 78,481 + -0.1 78,482 - -0.5 78,482 - +1.0 78,482 - +0.6 78,482 - +0.3 78,482 - -0.8 78,582 - +0.0 78,734 + RR42_RS21190 0.21 +0.5 78,735 - RR42_RS21190 0.21 +0.8 78,735 - RR42_RS21190 0.21 +0.8 78,735 - RR42_RS21190 0.21 +0.1 78,784 + RR42_RS21190 0.32 +0.3 78,784 + RR42_RS21190 0.32 +0.0 78,785 - RR42_RS21190 0.32 -1.1 78,785 - RR42_RS21190 0.32 -0.2 78,823 + RR42_RS21190 0.41 +2.2 78,824 - RR42_RS21190 0.41 -1.0 78,971 - RR42_RS21190 0.74 -0.6 79,096 + +0.2 79,097 - -0.9 79,097 - +0.9 79,097 - -0.7 79,097 - -0.8 79,106 - -0.0 79,159 - +1.0 79,310 + RR42_RS21195 0.23 +1.4 79,364 + RR42_RS21195 0.30 -0.2 79,439 + RR42_RS21195 0.41 -0.4 79,439 + RR42_RS21195 0.41 +0.5 79,439 + RR42_RS21195 0.41 +1.8 79,439 + RR42_RS21195 0.41 -2.2 79,439 + RR42_RS21195 0.41 +0.8 79,439 + RR42_RS21195 0.41 +0.3 79,440 - RR42_RS21195 0.41 -3.2 79,440 - RR42_RS21195 0.41 +1.0 79,724 + RR42_RS21195 0.82 +1.5 79,724 + RR42_RS21195 0.82 -0.2 79,795 + +1.5 79,796 - -1.0 79,796 - -2.3 79,890 - -0.8 79,890 - -0.4 80,043 + RR42_RS21200 0.22 -0.2 80,044 - RR42_RS21200 0.22 +0.0 80,084 - RR42_RS21200 0.28 -0.2 80,154 + RR42_RS21200 0.39 -0.4 80,154 + RR42_RS21200 0.39 -1.7 80,155 - RR42_RS21200 0.39 -1.3 80,155 - RR42_RS21200 0.39 +0.0 80,155 - RR42_RS21200 0.39 +0.7 80,188 - RR42_RS21200 0.44 +1.0 80,247 + RR42_RS21200 0.54 -0.7 80,311 - RR42_RS21200 0.64 +0.8 80,410 - RR42_RS21200 0.79 -0.3 80,529 + +0.2 80,530 - +0.5 80,530 - +0.5 80,727 - -0.0 80,742 + -2.1
Or see this region's nucleotide sequence