Experiment: R2A with Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21115 and RR42_RS21120 are separated by 137 nucleotides RR42_RS21120 and RR42_RS21125 are separated by 249 nucleotides
RR42_RS21115: RR42_RS21115 - flagellin, at 62,515 to 63,195
_RS21115
RR42_RS21120: RR42_RS21120 - acetate kinase, at 63,333 to 64,745
_RS21120
RR42_RS21125: RR42_RS21125 - membrane protein, at 64,995 to 65,507
_RS21125
Position (kb) 63
64
65 Strain fitness (log2 ratio) -2
-1
0
1
2 at 62.382 kb on - strand at 62.492 kb on + strand at 62.493 kb on - strand at 62.703 kb on + strand, within RR42_RS21115 at 62.703 kb on + strand, within RR42_RS21115 at 62.704 kb on - strand, within RR42_RS21115 at 62.704 kb on - strand, within RR42_RS21115 at 62.704 kb on - strand, within RR42_RS21115 at 62.706 kb on + strand, within RR42_RS21115 at 62.706 kb on + strand, within RR42_RS21115 at 62.707 kb on - strand, within RR42_RS21115 at 62.707 kb on - strand, within RR42_RS21115 at 62.851 kb on - strand, within RR42_RS21115 at 62.871 kb on + strand, within RR42_RS21115 at 62.872 kb on - strand, within RR42_RS21115 at 62.872 kb on - strand, within RR42_RS21115 at 62.872 kb on - strand, within RR42_RS21115 at 62.993 kb on + strand, within RR42_RS21115 at 62.993 kb on + strand, within RR42_RS21115 at 62.993 kb on + strand, within RR42_RS21115 at 63.171 kb on - strand at 63.201 kb on - strand at 63.201 kb on - strand at 63.239 kb on - strand at 63.241 kb on + strand at 63.241 kb on + strand at 63.241 kb on + strand at 63.241 kb on + strand at 63.245 kb on + strand at 63.245 kb on + strand at 63.245 kb on + strand at 63.246 kb on - strand at 63.246 kb on - strand at 63.269 kb on + strand at 63.270 kb on - strand at 63.880 kb on - strand, within RR42_RS21120 at 63.880 kb on - strand, within RR42_RS21120 at 63.975 kb on + strand, within RR42_RS21120 at 63.975 kb on + strand, within RR42_RS21120 at 63.975 kb on + strand, within RR42_RS21120 at 63.976 kb on - strand, within RR42_RS21120 at 63.976 kb on - strand, within RR42_RS21120 at 64.025 kb on - strand, within RR42_RS21120 at 64.025 kb on - strand, within RR42_RS21120 at 64.025 kb on - strand, within RR42_RS21120 at 64.025 kb on - strand, within RR42_RS21120 at 64.042 kb on - strand, within RR42_RS21120 at 64.155 kb on + strand, within RR42_RS21120 at 64.167 kb on + strand, within RR42_RS21120 at 64.168 kb on - strand, within RR42_RS21120 at 64.168 kb on - strand, within RR42_RS21120 at 64.209 kb on + strand, within RR42_RS21120 at 64.210 kb on - strand, within RR42_RS21120 at 64.210 kb on - strand, within RR42_RS21120 at 64.347 kb on - strand, within RR42_RS21120 at 64.395 kb on + strand, within RR42_RS21120 at 64.395 kb on + strand, within RR42_RS21120 at 64.395 kb on + strand, within RR42_RS21120 at 64.635 kb on + strand at 64.660 kb on - strand at 64.827 kb on - strand at 64.827 kb on - strand at 64.842 kb on + strand at 64.843 kb on - strand at 65.006 kb on + strand at 65.006 kb on + strand at 65.007 kb on - strand at 65.007 kb on - strand at 65.007 kb on - strand at 65.117 kb on + strand, within RR42_RS21125 at 65.118 kb on - strand, within RR42_RS21125 at 65.614 kb on - strand at 65.681 kb on + strand at 65.682 kb on - strand at 65.682 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml remove 62,382 - +0.8 62,492 + -1.1 62,493 - +0.5 62,703 + RR42_RS21115 0.28 -1.3 62,703 + RR42_RS21115 0.28 +0.0 62,704 - RR42_RS21115 0.28 -0.3 62,704 - RR42_RS21115 0.28 -0.2 62,704 - RR42_RS21115 0.28 +1.0 62,706 + RR42_RS21115 0.28 +0.6 62,706 + RR42_RS21115 0.28 -0.1 62,707 - RR42_RS21115 0.28 +0.2 62,707 - RR42_RS21115 0.28 +0.1 62,851 - RR42_RS21115 0.49 -1.0 62,871 + RR42_RS21115 0.52 -0.5 62,872 - RR42_RS21115 0.52 -1.7 62,872 - RR42_RS21115 0.52 -1.7 62,872 - RR42_RS21115 0.52 -0.1 62,993 + RR42_RS21115 0.70 +0.0 62,993 + RR42_RS21115 0.70 -0.5 62,993 + RR42_RS21115 0.70 -0.2 63,171 - +0.4 63,201 - -0.5 63,201 - -0.7 63,239 - +0.8 63,241 + +0.3 63,241 + +0.5 63,241 + -1.0 63,241 + +0.3 63,245 + -1.2 63,245 + +1.3 63,245 + +1.4 63,246 - +0.3 63,246 - +0.0 63,269 + -0.8 63,270 - -2.0 63,880 - RR42_RS21120 0.39 -0.2 63,880 - RR42_RS21120 0.39 +1.9 63,975 + RR42_RS21120 0.45 +0.2 63,975 + RR42_RS21120 0.45 +0.1 63,975 + RR42_RS21120 0.45 +0.5 63,976 - RR42_RS21120 0.46 +0.5 63,976 - RR42_RS21120 0.46 -0.2 64,025 - RR42_RS21120 0.49 +0.5 64,025 - RR42_RS21120 0.49 -0.8 64,025 - RR42_RS21120 0.49 +0.9 64,025 - RR42_RS21120 0.49 +0.4 64,042 - RR42_RS21120 0.50 -1.8 64,155 + RR42_RS21120 0.58 +2.2 64,167 + RR42_RS21120 0.59 -0.8 64,168 - RR42_RS21120 0.59 +0.2 64,168 - RR42_RS21120 0.59 -0.6 64,209 + RR42_RS21120 0.62 -0.3 64,210 - RR42_RS21120 0.62 -0.1 64,210 - RR42_RS21120 0.62 -0.1 64,347 - RR42_RS21120 0.72 +0.8 64,395 + RR42_RS21120 0.75 -0.1 64,395 + RR42_RS21120 0.75 +0.6 64,395 + RR42_RS21120 0.75 +1.0 64,635 + +0.1 64,660 - -0.1 64,827 - -0.4 64,827 - +0.3 64,842 + -0.8 64,843 - +0.5 65,006 + -1.0 65,006 + +0.2 65,007 - -0.0 65,007 - +0.1 65,007 - -0.5 65,117 + RR42_RS21125 0.24 -0.5 65,118 - RR42_RS21125 0.24 +0.8 65,614 - -0.0 65,681 + -0.8 65,682 - +0.4 65,682 - +0.6
Or see this region's nucleotide sequence