Experiment: R2A with Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS20340 and RR42_RS20345 are separated by 12 nucleotides RR42_RS20345 and RR42_RS20350 overlap by 4 nucleotides RR42_RS20350 and RR42_RS20355 overlap by 4 nucleotides
RR42_RS20340: RR42_RS20340 - LysR family transcriptional regulator, at 4,399,982 to 4,400,908
_RS20340
RR42_RS20345: RR42_RS20345 - penicillin-binding protein, at 4,400,921 to 4,403,125
_RS20345
RR42_RS20350: RR42_RS20350 - hypothetical protein, at 4,403,122 to 4,403,823
_RS20350
RR42_RS20355: RR42_RS20355 - alpha-2-macroglobulin, at 4,403,820 to 4,409,840
_RS20355
Position (kb)
4400
4401
4402
4403
4404 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4399.968 kb on - strand at 4400.142 kb on + strand, within RR42_RS20340 at 4400.519 kb on + strand, within RR42_RS20340 at 4400.520 kb on - strand, within RR42_RS20340 at 4400.582 kb on + strand, within RR42_RS20340 at 4400.583 kb on - strand, within RR42_RS20340 at 4400.609 kb on + strand, within RR42_RS20340 at 4400.610 kb on - strand, within RR42_RS20340 at 4400.817 kb on - strand at 4400.817 kb on - strand at 4400.817 kb on - strand at 4400.817 kb on - strand at 4400.822 kb on + strand at 4400.822 kb on + strand at 4400.823 kb on - strand at 4400.823 kb on - strand at 4400.906 kb on + strand at 4400.907 kb on - strand at 4401.266 kb on - strand, within RR42_RS20345 at 4401.481 kb on - strand, within RR42_RS20345 at 4401.631 kb on - strand, within RR42_RS20345 at 4401.729 kb on + strand, within RR42_RS20345 at 4401.790 kb on - strand, within RR42_RS20345 at 4401.790 kb on - strand, within RR42_RS20345 at 4401.792 kb on + strand, within RR42_RS20345 at 4401.793 kb on - strand, within RR42_RS20345 at 4401.885 kb on - strand, within RR42_RS20345 at 4401.933 kb on + strand, within RR42_RS20345 at 4401.934 kb on - strand, within RR42_RS20345 at 4401.960 kb on + strand, within RR42_RS20345 at 4401.960 kb on + strand, within RR42_RS20345 at 4401.961 kb on - strand, within RR42_RS20345 at 4401.961 kb on - strand, within RR42_RS20345 at 4402.054 kb on - strand, within RR42_RS20345 at 4402.054 kb on - strand, within RR42_RS20345 at 4402.146 kb on + strand, within RR42_RS20345 at 4402.146 kb on + strand, within RR42_RS20345 at 4402.146 kb on + strand, within RR42_RS20345 at 4402.147 kb on - strand, within RR42_RS20345 at 4402.322 kb on + strand, within RR42_RS20345 at 4402.323 kb on - strand, within RR42_RS20345 at 4402.560 kb on + strand, within RR42_RS20345 at 4402.561 kb on - strand, within RR42_RS20345 at 4402.561 kb on - strand, within RR42_RS20345 at 4402.633 kb on - strand, within RR42_RS20345 at 4402.861 kb on - strand, within RR42_RS20345 at 4402.861 kb on - strand, within RR42_RS20345 at 4403.205 kb on + strand, within RR42_RS20350 at 4403.342 kb on + strand, within RR42_RS20350 at 4403.343 kb on - strand, within RR42_RS20350 at 4403.748 kb on - strand, within RR42_RS20350 at 4403.868 kb on + strand at 4403.868 kb on + strand at 4403.881 kb on + strand at 4403.881 kb on + strand at 4403.974 kb on + strand at 4403.974 kb on + strand at 4403.975 kb on - strand at 4403.980 kb on + strand at 4403.981 kb on - strand at 4403.981 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml remove 4,399,968 - +0.5 4,400,142 + RR42_RS20340 0.17 -0.5 4,400,519 + RR42_RS20340 0.58 +0.5 4,400,520 - RR42_RS20340 0.58 -0.4 4,400,582 + RR42_RS20340 0.65 -0.5 4,400,583 - RR42_RS20340 0.65 -1.0 4,400,609 + RR42_RS20340 0.68 +3.4 4,400,610 - RR42_RS20340 0.68 -0.3 4,400,817 - +0.4 4,400,817 - -0.5 4,400,817 - +0.8 4,400,817 - -2.3 4,400,822 + -0.8 4,400,822 + +0.7 4,400,823 - -0.4 4,400,823 - +0.5 4,400,906 + -0.1 4,400,907 - -0.1 4,401,266 - RR42_RS20345 0.16 -2.2 4,401,481 - RR42_RS20345 0.25 -0.8 4,401,631 - RR42_RS20345 0.32 -0.7 4,401,729 + RR42_RS20345 0.37 +0.0 4,401,790 - RR42_RS20345 0.39 -2.5 4,401,790 - RR42_RS20345 0.39 +1.5 4,401,792 + RR42_RS20345 0.40 -0.4 4,401,793 - RR42_RS20345 0.40 -0.5 4,401,885 - RR42_RS20345 0.44 +0.2 4,401,933 + RR42_RS20345 0.46 +0.1 4,401,934 - RR42_RS20345 0.46 +1.8 4,401,960 + RR42_RS20345 0.47 -1.7 4,401,960 + RR42_RS20345 0.47 +2.1 4,401,961 - RR42_RS20345 0.47 -1.3 4,401,961 - RR42_RS20345 0.47 -0.6 4,402,054 - RR42_RS20345 0.51 +0.5 4,402,054 - RR42_RS20345 0.51 -1.0 4,402,146 + RR42_RS20345 0.56 +1.1 4,402,146 + RR42_RS20345 0.56 -0.4 4,402,146 + RR42_RS20345 0.56 +0.9 4,402,147 - RR42_RS20345 0.56 -0.5 4,402,322 + RR42_RS20345 0.64 -1.3 4,402,323 - RR42_RS20345 0.64 +0.1 4,402,560 + RR42_RS20345 0.74 -1.0 4,402,561 - RR42_RS20345 0.74 -2.3 4,402,561 - RR42_RS20345 0.74 -1.6 4,402,633 - RR42_RS20345 0.78 -0.7 4,402,861 - RR42_RS20345 0.88 +0.8 4,402,861 - RR42_RS20345 0.88 -0.6 4,403,205 + RR42_RS20350 0.12 +0.5 4,403,342 + RR42_RS20350 0.31 +0.6 4,403,343 - RR42_RS20350 0.31 +0.5 4,403,748 - RR42_RS20350 0.89 -1.1 4,403,868 + +1.0 4,403,868 + +0.5 4,403,881 + -0.4 4,403,881 + +0.7 4,403,974 + -0.3 4,403,974 + -0.3 4,403,975 - +1.1 4,403,980 + +0.2 4,403,981 - -0.4 4,403,981 - +0.0
Or see this region's nucleotide sequence