Experiment: R2A with Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS20125 and RR42_RS20130 are separated by 203 nucleotides RR42_RS20130 and RR42_RS20135 are separated by 574 nucleotides
RR42_RS20125: RR42_RS20125 - L-glutamate gamma-semialdehyde dehydrogenase (EC 1.2.1.88); Proline dehydrogenase (EC 1.5.5.2) (from data) , at 4,349,292 to 4,353,293
_RS20125
RR42_RS20130: RR42_RS20130 - primosome assembly protein PriA, at 4,353,497 to 4,355,800
_RS20130
RR42_RS20135: RR42_RS20135 - uroporphyrinogen decarboxylase, at 4,356,375 to 4,357,472
_RS20135
Position (kb)
4353
4354
4355
4356 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4352.618 kb on + strand, within RR42_RS20125 at 4352.618 kb on + strand, within RR42_RS20125 at 4352.619 kb on - strand, within RR42_RS20125 at 4352.619 kb on - strand, within RR42_RS20125 at 4353.057 kb on + strand at 4353.175 kb on + strand at 4353.175 kb on + strand at 4353.176 kb on - strand at 4353.309 kb on + strand at 4353.310 kb on - strand at 4353.310 kb on - strand at 4353.310 kb on - strand at 4353.365 kb on + strand at 4353.365 kb on + strand at 4353.366 kb on - strand at 4353.392 kb on + strand at 4353.396 kb on + strand at 4353.397 kb on - strand at 4355.843 kb on + strand at 4355.844 kb on - strand at 4355.937 kb on + strand at 4355.937 kb on + strand at 4355.938 kb on - strand at 4355.938 kb on - strand at 4355.943 kb on - strand at 4355.962 kb on + strand at 4355.963 kb on - strand at 4355.973 kb on + strand at 4355.973 kb on + strand at 4355.973 kb on + strand at 4355.973 kb on + strand at 4355.973 kb on + strand at 4355.973 kb on + strand at 4355.973 kb on + strand at 4355.973 kb on + strand at 4355.974 kb on - strand at 4355.974 kb on - strand at 4355.974 kb on - strand at 4355.974 kb on - strand at 4356.014 kb on - strand at 4356.014 kb on - strand at 4356.111 kb on + strand at 4356.112 kb on - strand at 4356.192 kb on + strand at 4356.192 kb on + strand at 4356.193 kb on - strand at 4356.193 kb on - strand at 4356.237 kb on + strand at 4356.247 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml remove 4,352,618 + RR42_RS20125 0.83 +0.7 4,352,618 + RR42_RS20125 0.83 +0.1 4,352,619 - RR42_RS20125 0.83 -2.0 4,352,619 - RR42_RS20125 0.83 -0.2 4,353,057 + +1.3 4,353,175 + -0.3 4,353,175 + +0.0 4,353,176 - -0.2 4,353,309 + +3.1 4,353,310 - -0.2 4,353,310 - +0.0 4,353,310 - +0.9 4,353,365 + -1.1 4,353,365 + +0.4 4,353,366 - -0.0 4,353,392 + -0.7 4,353,396 + -0.9 4,353,397 - +0.1 4,355,843 + +1.3 4,355,844 - -0.0 4,355,937 + -2.5 4,355,937 + -1.7 4,355,938 - +2.1 4,355,938 - -0.0 4,355,943 - -1.9 4,355,962 + -0.5 4,355,963 - -1.0 4,355,973 + -0.8 4,355,973 + -0.9 4,355,973 + +1.1 4,355,973 + -0.0 4,355,973 + +0.5 4,355,973 + +0.7 4,355,973 + +1.1 4,355,973 + -2.6 4,355,974 - -0.7 4,355,974 - +0.8 4,355,974 - +0.2 4,355,974 - +0.3 4,356,014 - +0.6 4,356,014 - -0.9 4,356,111 + -0.8 4,356,112 - +0.7 4,356,192 + -1.2 4,356,192 + +1.5 4,356,193 - -0.4 4,356,193 - -1.0 4,356,237 + +0.2 4,356,247 + -0.7
Or see this region's nucleotide sequence