Experiment: R2A with Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS17530 and RR42_RS17535 are separated by 116 nucleotides RR42_RS17535 and RR42_RS17540 are separated by 64 nucleotides RR42_RS17540 and RR42_RS17545 are separated by 64 nucleotides
RR42_RS17530: RR42_RS17530 - gamma-glutamyl phosphate reductase, at 3,792,986 to 3,794,266
_RS17530
RR42_RS17535: RR42_RS17535 - DNA polymerase III subunit delta, at 3,794,383 to 3,795,423
_RS17535
RR42_RS17540: RR42_RS17540 - lipoprotein, at 3,795,488 to 3,795,994
_RS17540
RR42_RS17545: RR42_RS17545 - leucyl-tRNA synthetase, at 3,796,059 to 3,798,695
_RS17545
Position (kb)
3794
3795
3796 Strain fitness (log2 ratio)
-1
0
1
2 at 3793.413 kb on + strand, within RR42_RS17530 at 3793.414 kb on - strand, within RR42_RS17530 at 3793.560 kb on + strand, within RR42_RS17530 at 3793.561 kb on - strand, within RR42_RS17530 at 3793.561 kb on - strand, within RR42_RS17530 at 3793.561 kb on - strand, within RR42_RS17530 at 3793.561 kb on - strand, within RR42_RS17530 at 3793.561 kb on - strand, within RR42_RS17530 at 3793.561 kb on - strand, within RR42_RS17530 at 3793.666 kb on - strand, within RR42_RS17530 at 3794.242 kb on - strand at 3794.242 kb on - strand at 3794.242 kb on - strand at 3794.242 kb on - strand at 3794.325 kb on - strand at 3794.325 kb on - strand at 3796.044 kb on - strand at 3796.048 kb on - strand at 3796.048 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml remove 3,793,413 + RR42_RS17530 0.33 +0.6 3,793,414 - RR42_RS17530 0.33 -0.7 3,793,560 + RR42_RS17530 0.45 -0.5 3,793,561 - RR42_RS17530 0.45 +0.5 3,793,561 - RR42_RS17530 0.45 -0.2 3,793,561 - RR42_RS17530 0.45 -1.5 3,793,561 - RR42_RS17530 0.45 +1.3 3,793,561 - RR42_RS17530 0.45 +0.7 3,793,561 - RR42_RS17530 0.45 +0.9 3,793,666 - RR42_RS17530 0.53 -0.2 3,794,242 - +2.1 3,794,242 - +0.8 3,794,242 - +0.3 3,794,242 - -1.0 3,794,325 - -0.9 3,794,325 - +0.9 3,796,044 - -1.5 3,796,048 - +0.5 3,796,048 - -1.1
Or see this region's nucleotide sequence