Experiment: R2A with Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS17310 and RR42_RS17315 are separated by 65 nucleotides RR42_RS17315 and RR42_RS17320 are separated by 164 nucleotides RR42_RS17320 and RR42_RS17325 overlap by 4 nucleotides
RR42_RS17310: RR42_RS17310 - D-lactate dehydrogenase, FAD binding subunit GlcE (EC 1.1.99.6) (from data) , at 3,748,459 to 3,749,583
_RS17310
RR42_RS17315: RR42_RS17315 - D-lactate dehydrogenase, iron-sulfur subunit GlcF (EC 1.1.99.6) (from data) , at 3,749,649 to 3,750,914
_RS17315
RR42_RS17320: RR42_RS17320 - murein hydrolase transporter LrgA, at 3,751,079 to 3,751,465
_RS17320
RR42_RS17325: RR42_RS17325 - membrane protein, at 3,751,462 to 3,752,184
_RS17325
Position (kb)
3749
3750
3751 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3748.927 kb on + strand, within RR42_RS17310 at 3748.928 kb on - strand, within RR42_RS17310 at 3749.569 kb on + strand at 3749.569 kb on + strand at 3749.569 kb on + strand at 3749.569 kb on + strand at 3749.569 kb on + strand at 3749.570 kb on - strand at 3749.570 kb on - strand at 3749.682 kb on - strand at 3749.760 kb on + strand at 3749.761 kb on - strand at 3749.761 kb on - strand at 3749.761 kb on - strand at 3749.805 kb on + strand, within RR42_RS17315 at 3749.805 kb on + strand, within RR42_RS17315 at 3749.806 kb on - strand, within RR42_RS17315 at 3749.806 kb on - strand, within RR42_RS17315 at 3749.953 kb on + strand, within RR42_RS17315 at 3749.953 kb on + strand, within RR42_RS17315 at 3749.954 kb on - strand, within RR42_RS17315 at 3750.335 kb on + strand, within RR42_RS17315 at 3750.335 kb on + strand, within RR42_RS17315 at 3750.336 kb on - strand, within RR42_RS17315 at 3750.336 kb on - strand, within RR42_RS17315 at 3750.336 kb on - strand, within RR42_RS17315 at 3750.433 kb on - strand at 3750.462 kb on + strand, within RR42_RS17315 at 3750.606 kb on + strand, within RR42_RS17315 at 3750.606 kb on + strand, within RR42_RS17315 at 3750.607 kb on - strand, within RR42_RS17315 at 3750.735 kb on + strand, within RR42_RS17315 at 3750.736 kb on - strand, within RR42_RS17315 at 3750.736 kb on - strand, within RR42_RS17315 at 3750.736 kb on - strand, within RR42_RS17315 at 3750.763 kb on - strand, within RR42_RS17315 at 3750.830 kb on + strand at 3750.913 kb on - strand at 3750.913 kb on - strand at 3750.913 kb on - strand at 3751.136 kb on + strand, within RR42_RS17320 at 3751.136 kb on + strand, within RR42_RS17320 at 3751.137 kb on - strand, within RR42_RS17320 at 3751.492 kb on + strand at 3751.493 kb on - strand at 3751.527 kb on + strand at 3751.540 kb on + strand, within RR42_RS17325 at 3751.561 kb on + strand, within RR42_RS17325 at 3751.561 kb on + strand, within RR42_RS17325 at 3751.562 kb on - strand, within RR42_RS17325 at 3751.562 kb on - strand, within RR42_RS17325 at 3751.651 kb on + strand, within RR42_RS17325 at 3751.660 kb on + strand, within RR42_RS17325 at 3751.660 kb on + strand, within RR42_RS17325 at 3751.660 kb on + strand, within RR42_RS17325 at 3751.661 kb on - strand, within RR42_RS17325 at 3751.661 kb on - strand, within RR42_RS17325 at 3751.661 kb on - strand, within RR42_RS17325 at 3751.661 kb on - strand, within RR42_RS17325 at 3751.729 kb on + strand, within RR42_RS17325
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml remove 3,748,927 + RR42_RS17310 0.42 +1.3 3,748,928 - RR42_RS17310 0.42 +0.8 3,749,569 + +2.1 3,749,569 + +0.4 3,749,569 + -0.5 3,749,569 + +0.5 3,749,569 + +0.4 3,749,570 - +0.3 3,749,570 - -0.1 3,749,682 - -0.7 3,749,760 + +0.8 3,749,761 - +2.1 3,749,761 - -0.5 3,749,761 - +0.7 3,749,805 + RR42_RS17315 0.12 -0.7 3,749,805 + RR42_RS17315 0.12 +1.1 3,749,806 - RR42_RS17315 0.12 -0.1 3,749,806 - RR42_RS17315 0.12 -1.5 3,749,953 + RR42_RS17315 0.24 -0.2 3,749,953 + RR42_RS17315 0.24 -0.5 3,749,954 - RR42_RS17315 0.24 +0.4 3,750,335 + RR42_RS17315 0.54 +1.5 3,750,335 + RR42_RS17315 0.54 -2.0 3,750,336 - RR42_RS17315 0.54 +0.8 3,750,336 - RR42_RS17315 0.54 +0.2 3,750,336 - RR42_RS17315 0.54 -0.4 3,750,433 - +2.1 3,750,462 + RR42_RS17315 0.64 -0.1 3,750,606 + RR42_RS17315 0.76 -1.9 3,750,606 + RR42_RS17315 0.76 -1.0 3,750,607 - RR42_RS17315 0.76 -1.1 3,750,735 + RR42_RS17315 0.86 -0.4 3,750,736 - RR42_RS17315 0.86 -0.5 3,750,736 - RR42_RS17315 0.86 -0.2 3,750,736 - RR42_RS17315 0.86 -0.6 3,750,763 - RR42_RS17315 0.88 -1.0 3,750,830 + +1.0 3,750,913 - +0.5 3,750,913 - -0.2 3,750,913 - +0.1 3,751,136 + RR42_RS17320 0.15 +0.2 3,751,136 + RR42_RS17320 0.15 +0.3 3,751,137 - RR42_RS17320 0.15 +0.7 3,751,492 + +1.5 3,751,493 - +0.8 3,751,527 + +1.3 3,751,540 + RR42_RS17325 0.11 -0.9 3,751,561 + RR42_RS17325 0.14 -0.5 3,751,561 + RR42_RS17325 0.14 -2.4 3,751,562 - RR42_RS17325 0.14 -0.1 3,751,562 - RR42_RS17325 0.14 +0.5 3,751,651 + RR42_RS17325 0.26 +0.3 3,751,660 + RR42_RS17325 0.27 -0.8 3,751,660 + RR42_RS17325 0.27 +0.5 3,751,660 + RR42_RS17325 0.27 -0.2 3,751,661 - RR42_RS17325 0.28 -0.0 3,751,661 - RR42_RS17325 0.28 +0.8 3,751,661 - RR42_RS17325 0.28 +0.9 3,751,661 - RR42_RS17325 0.28 -0.9 3,751,729 + RR42_RS17325 0.37 -0.2
Or see this region's nucleotide sequence