Experiment: R2A with Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15215 and RR42_RS15220 are separated by 214 nucleotides RR42_RS15220 and RR42_RS15225 are separated by 4 nucleotides RR42_RS15225 and RR42_RS15230 are separated by 79 nucleotides RR42_RS15230 and RR42_RS15235 are separated by 11 nucleotides
RR42_RS15215: RR42_RS15215 - carnitine dehydratase, at 3,295,254 to 3,296,693
_RS15215
RR42_RS15220: RR42_RS15220 - glutaminyl-tRNA synthetase, at 3,296,908 to 3,298,686
_RS15220
RR42_RS15225: RR42_RS15225 - RNA-binding protein, at 3,298,691 to 3,298,909
_RS15225
RR42_RS15230: RR42_RS15230 - membrane protein, at 3,298,989 to 3,299,372
_RS15230
RR42_RS15235: RR42_RS15235 - hypothetical protein, at 3,299,384 to 3,299,986
_RS15235
Position (kb) 3296
3297
3298
3299 Strain fitness (log2 ratio) -1
0
1
2 at 3296.329 kb on - strand, within RR42_RS15215 at 3296.410 kb on + strand, within RR42_RS15215 at 3296.678 kb on + strand at 3296.678 kb on + strand at 3296.734 kb on + strand at 3296.735 kb on - strand at 3298.685 kb on - strand at 3298.685 kb on - strand at 3298.726 kb on + strand, within RR42_RS15225 at 3299.265 kb on + strand, within RR42_RS15230 at 3299.266 kb on - strand, within RR42_RS15230 at 3299.266 kb on - strand, within RR42_RS15230 at 3299.277 kb on + strand, within RR42_RS15230 at 3299.277 kb on + strand, within RR42_RS15230 at 3299.343 kb on - strand at 3299.343 kb on - strand at 3299.498 kb on + strand, within RR42_RS15235 at 3299.498 kb on + strand, within RR42_RS15235 at 3299.499 kb on - strand, within RR42_RS15235 at 3299.499 kb on - strand, within RR42_RS15235 at 3299.499 kb on - strand, within RR42_RS15235
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml remove 3,296,329 - RR42_RS15215 0.75 +2.1 3,296,410 + RR42_RS15215 0.80 -0.9 3,296,678 + -1.0 3,296,678 + +0.5 3,296,734 + -0.1 3,296,735 - -0.2 3,298,685 - -1.1 3,298,685 - +0.9 3,298,726 + RR42_RS15225 0.16 -0.5 3,299,265 + RR42_RS15230 0.72 -1.0 3,299,266 - RR42_RS15230 0.72 -0.2 3,299,266 - RR42_RS15230 0.72 -0.1 3,299,277 + RR42_RS15230 0.75 -1.3 3,299,277 + RR42_RS15230 0.75 -0.2 3,299,343 - -0.0 3,299,343 - -0.6 3,299,498 + RR42_RS15235 0.19 -0.3 3,299,498 + RR42_RS15235 0.19 -0.1 3,299,499 - RR42_RS15235 0.19 +0.1 3,299,499 - RR42_RS15235 0.19 +0.5 3,299,499 - RR42_RS15235 0.19 +0.6
Or see this region's nucleotide sequence