Experiment: R2A with Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS13535 and RR42_RS13540 are separated by 71 nucleotides RR42_RS13540 and RR42_RS13545 are separated by 15 nucleotides RR42_RS13545 and RR42_RS13550 are separated by 52 nucleotides
RR42_RS13535: RR42_RS13535 - 2-aminobenzoate-CoA ligase (EC 6.2.1.32) (from data) , at 2,920,892 to 2,922,565
_RS13535
RR42_RS13540: RR42_RS13540 - acyl-CoA dehydrogenase, at 2,922,637 to 2,923,800
_RS13540
RR42_RS13545: RR42_RS13545 - enoyl-CoA hydratase, at 2,923,816 to 2,924,667
_RS13545
RR42_RS13550: RR42_RS13550 - 3-hydroxyacyl-CoA dehydrogenase, at 2,924,720 to 2,925,520
_RS13550
Position (kb)
2922
2923
2924 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2921.659 kb on - strand, within RR42_RS13535 at 2921.659 kb on - strand, within RR42_RS13535 at 2921.800 kb on + strand, within RR42_RS13535 at 2921.801 kb on - strand, within RR42_RS13535 at 2921.801 kb on - strand, within RR42_RS13535 at 2921.830 kb on + strand, within RR42_RS13535 at 2921.831 kb on - strand, within RR42_RS13535 at 2921.831 kb on - strand, within RR42_RS13535 at 2921.956 kb on + strand, within RR42_RS13535 at 2921.957 kb on - strand, within RR42_RS13535 at 2922.046 kb on - strand, within RR42_RS13535 at 2922.358 kb on - strand, within RR42_RS13535 at 2922.358 kb on - strand, within RR42_RS13535 at 2922.537 kb on + strand at 2922.537 kb on + strand at 2922.537 kb on + strand at 2922.537 kb on + strand at 2922.538 kb on - strand at 2922.542 kb on - strand at 2922.542 kb on - strand at 2922.542 kb on - strand at 2922.704 kb on + strand at 2922.704 kb on + strand at 2922.705 kb on - strand at 2922.731 kb on + strand at 2922.893 kb on + strand, within RR42_RS13540 at 2923.289 kb on + strand, within RR42_RS13540 at 2923.290 kb on - strand, within RR42_RS13540 at 2923.808 kb on + strand at 2923.809 kb on - strand at 2923.809 kb on - strand at 2923.809 kb on - strand at 2923.809 kb on - strand at 2923.863 kb on - strand at 2924.656 kb on + strand at 2924.767 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml remove 2,921,659 - RR42_RS13535 0.46 -0.1 2,921,659 - RR42_RS13535 0.46 +0.4 2,921,800 + RR42_RS13535 0.54 +0.4 2,921,801 - RR42_RS13535 0.54 -1.4 2,921,801 - RR42_RS13535 0.54 +1.1 2,921,830 + RR42_RS13535 0.56 +0.1 2,921,831 - RR42_RS13535 0.56 -1.8 2,921,831 - RR42_RS13535 0.56 +0.5 2,921,956 + RR42_RS13535 0.64 +0.3 2,921,957 - RR42_RS13535 0.64 +0.3 2,922,046 - RR42_RS13535 0.69 +1.0 2,922,358 - RR42_RS13535 0.88 -0.7 2,922,358 - RR42_RS13535 0.88 -0.3 2,922,537 + -0.4 2,922,537 + -0.9 2,922,537 + -0.7 2,922,537 + +0.3 2,922,538 - +1.5 2,922,542 - +0.1 2,922,542 - -0.9 2,922,542 - +0.1 2,922,704 + +1.9 2,922,704 + +0.4 2,922,705 - +0.3 2,922,731 + +0.9 2,922,893 + RR42_RS13540 0.22 +0.1 2,923,289 + RR42_RS13540 0.56 +0.2 2,923,290 - RR42_RS13540 0.56 +0.6 2,923,808 + +0.3 2,923,809 - -2.4 2,923,809 - -0.0 2,923,809 - +0.2 2,923,809 - +0.4 2,923,863 - -1.0 2,924,656 + -0.1 2,924,767 + +0.5
Or see this region's nucleotide sequence