Experiment: R2A with Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12035 and RR42_RS12040 are separated by 474 nucleotides RR42_RS12040 and RR42_RS12045 are separated by 24 nucleotides
RR42_RS12035: RR42_RS12035 - alanine racemase, at 2,579,675 to 2,580,790
_RS12035
RR42_RS12040: RR42_RS12040 - MFS transporter, at 2,581,265 to 2,582,584
_RS12040
RR42_RS12045: RR42_RS12045 - hypothetical protein, at 2,582,609 to 2,583,775
_RS12045
Position (kb)
2581
2582
2583 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2580.803 kb on - strand at 2580.803 kb on - strand at 2580.813 kb on - strand at 2580.815 kb on + strand at 2580.920 kb on + strand at 2580.962 kb on + strand at 2580.962 kb on + strand at 2580.963 kb on - strand at 2580.963 kb on - strand at 2580.963 kb on - strand at 2581.061 kb on - strand at 2581.062 kb on + strand at 2581.063 kb on - strand at 2581.063 kb on - strand at 2581.102 kb on + strand at 2581.102 kb on + strand at 2581.102 kb on + strand at 2581.189 kb on - strand at 2581.232 kb on + strand at 2581.233 kb on - strand at 2581.233 kb on - strand at 2581.233 kb on - strand at 2581.240 kb on + strand at 2581.253 kb on - strand at 2581.253 kb on - strand at 2581.264 kb on + strand at 2581.264 kb on + strand at 2581.264 kb on + strand at 2581.265 kb on - strand at 2581.265 kb on - strand at 2581.265 kb on - strand at 2581.280 kb on + strand at 2581.282 kb on + strand at 2581.282 kb on + strand at 2581.283 kb on - strand at 2581.407 kb on + strand, within RR42_RS12040 at 2581.416 kb on - strand, within RR42_RS12040 at 2581.416 kb on - strand, within RR42_RS12040 at 2581.416 kb on - strand, within RR42_RS12040 at 2581.416 kb on - strand, within RR42_RS12040 at 2581.501 kb on - strand, within RR42_RS12040 at 2581.547 kb on + strand, within RR42_RS12040 at 2581.577 kb on + strand, within RR42_RS12040 at 2581.593 kb on - strand, within RR42_RS12040 at 2581.597 kb on + strand, within RR42_RS12040 at 2581.717 kb on + strand, within RR42_RS12040 at 2581.717 kb on + strand, within RR42_RS12040 at 2581.751 kb on + strand, within RR42_RS12040 at 2581.812 kb on - strand, within RR42_RS12040 at 2581.812 kb on - strand, within RR42_RS12040 at 2581.827 kb on + strand, within RR42_RS12040 at 2581.919 kb on - strand, within RR42_RS12040 at 2581.919 kb on - strand, within RR42_RS12040 at 2582.192 kb on + strand, within RR42_RS12040 at 2582.252 kb on + strand, within RR42_RS12040 at 2582.252 kb on + strand, within RR42_RS12040 at 2582.253 kb on - strand, within RR42_RS12040 at 2582.253 kb on - strand, within RR42_RS12040 at 2582.253 kb on - strand, within RR42_RS12040 at 2582.268 kb on - strand, within RR42_RS12040 at 2582.303 kb on + strand, within RR42_RS12040 at 2582.303 kb on + strand, within RR42_RS12040 at 2582.426 kb on + strand, within RR42_RS12040 at 2582.427 kb on - strand, within RR42_RS12040 at 2582.528 kb on + strand at 2582.529 kb on - strand at 2582.582 kb on + strand at 2582.583 kb on - strand at 2582.583 kb on - strand at 2582.680 kb on + strand at 2583.001 kb on - strand, within RR42_RS12045 at 2583.021 kb on + strand, within RR42_RS12045 at 2583.021 kb on + strand, within RR42_RS12045 at 2583.021 kb on + strand, within RR42_RS12045 at 2583.021 kb on + strand, within RR42_RS12045 at 2583.103 kb on + strand, within RR42_RS12045
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml remove 2,580,803 - -0.9 2,580,803 - -1.0 2,580,813 - +0.3 2,580,815 + -1.2 2,580,920 + +1.0 2,580,962 + -0.3 2,580,962 + -0.3 2,580,963 - -0.2 2,580,963 - -1.4 2,580,963 - -0.5 2,581,061 - -1.3 2,581,062 + -0.4 2,581,063 - +0.4 2,581,063 - +2.0 2,581,102 + -1.3 2,581,102 + +1.3 2,581,102 + -1.9 2,581,189 - -0.8 2,581,232 + -0.0 2,581,233 - +0.2 2,581,233 - -1.9 2,581,233 - -2.1 2,581,240 + -1.6 2,581,253 - -1.9 2,581,253 - -1.6 2,581,264 + +0.3 2,581,264 + -0.3 2,581,264 + -0.7 2,581,265 - -1.5 2,581,265 - -0.7 2,581,265 - -1.2 2,581,280 + -1.0 2,581,282 + -0.0 2,581,282 + -0.6 2,581,283 - +0.4 2,581,407 + RR42_RS12040 0.11 -0.8 2,581,416 - RR42_RS12040 0.11 -0.8 2,581,416 - RR42_RS12040 0.11 -1.8 2,581,416 - RR42_RS12040 0.11 +0.4 2,581,416 - RR42_RS12040 0.11 -0.0 2,581,501 - RR42_RS12040 0.18 -0.8 2,581,547 + RR42_RS12040 0.21 -1.0 2,581,577 + RR42_RS12040 0.24 -3.0 2,581,593 - RR42_RS12040 0.25 -0.0 2,581,597 + RR42_RS12040 0.25 -3.0 2,581,717 + RR42_RS12040 0.34 -2.3 2,581,717 + RR42_RS12040 0.34 -0.3 2,581,751 + RR42_RS12040 0.37 -2.2 2,581,812 - RR42_RS12040 0.41 -1.5 2,581,812 - RR42_RS12040 0.41 -1.0 2,581,827 + RR42_RS12040 0.43 -2.3 2,581,919 - RR42_RS12040 0.50 -1.0 2,581,919 - RR42_RS12040 0.50 -0.6 2,582,192 + RR42_RS12040 0.70 +0.4 2,582,252 + RR42_RS12040 0.75 -2.8 2,582,252 + RR42_RS12040 0.75 +2.0 2,582,253 - RR42_RS12040 0.75 -1.7 2,582,253 - RR42_RS12040 0.75 -2.5 2,582,253 - RR42_RS12040 0.75 -1.2 2,582,268 - RR42_RS12040 0.76 +0.4 2,582,303 + RR42_RS12040 0.79 -3.4 2,582,303 + RR42_RS12040 0.79 -2.1 2,582,426 + RR42_RS12040 0.88 -0.9 2,582,427 - RR42_RS12040 0.88 +2.0 2,582,528 + -0.7 2,582,529 - +0.7 2,582,582 + -0.0 2,582,583 - +0.3 2,582,583 - +1.3 2,582,680 + +0.0 2,583,001 - RR42_RS12045 0.34 +0.9 2,583,021 + RR42_RS12045 0.35 +0.9 2,583,021 + RR42_RS12045 0.35 -0.2 2,583,021 + RR42_RS12045 0.35 -0.6 2,583,021 + RR42_RS12045 0.35 -0.9 2,583,103 + RR42_RS12045 0.42 +0.0
Or see this region's nucleotide sequence