Experiment: R2A with Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12020 and RR42_RS12025 are separated by 76 nucleotides RR42_RS12025 and RR42_RS12030 are separated by 82 nucleotides RR42_RS12030 and RR42_RS12035 are separated by 107 nucleotides
RR42_RS12020: RR42_RS12020 - GCN5 family acetyltransferase, at 2,576,935 to 2,577,471
_RS12020
RR42_RS12025: RR42_RS12025 - hypothetical protein, at 2,577,548 to 2,578,123
_RS12025
RR42_RS12030: RR42_RS12030 - DNA repair protein RadA, at 2,578,206 to 2,579,567
_RS12030
RR42_RS12035: RR42_RS12035 - alanine racemase, at 2,579,675 to 2,580,790
_RS12035
Position (kb)
2578
2579
2580 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2577.588 kb on + strand at 2577.589 kb on - strand at 2577.841 kb on + strand, within RR42_RS12025 at 2577.851 kb on + strand, within RR42_RS12025 at 2577.851 kb on + strand, within RR42_RS12025 at 2577.852 kb on - strand, within RR42_RS12025 at 2577.903 kb on + strand, within RR42_RS12025 at 2577.903 kb on + strand, within RR42_RS12025 at 2577.903 kb on + strand, within RR42_RS12025 at 2577.903 kb on + strand, within RR42_RS12025 at 2577.903 kb on + strand, within RR42_RS12025 at 2577.903 kb on + strand, within RR42_RS12025 at 2577.903 kb on + strand, within RR42_RS12025 at 2577.904 kb on - strand, within RR42_RS12025 at 2577.904 kb on - strand, within RR42_RS12025 at 2577.904 kb on - strand, within RR42_RS12025 at 2577.904 kb on - strand, within RR42_RS12025 at 2577.961 kb on - strand, within RR42_RS12025 at 2577.961 kb on - strand, within RR42_RS12025 at 2577.987 kb on + strand, within RR42_RS12025 at 2577.988 kb on - strand, within RR42_RS12025 at 2577.988 kb on - strand, within RR42_RS12025 at 2578.162 kb on - strand at 2579.212 kb on + strand, within RR42_RS12030 at 2579.543 kb on + strand at 2579.543 kb on + strand at 2579.543 kb on + strand at 2579.543 kb on + strand at 2579.543 kb on + strand at 2579.544 kb on - strand at 2579.544 kb on - strand at 2579.545 kb on + strand at 2579.545 kb on + strand at 2579.545 kb on + strand at 2579.546 kb on - strand at 2579.546 kb on - strand at 2579.546 kb on - strand at 2579.546 kb on - strand at 2579.562 kb on - strand at 2579.577 kb on + strand at 2579.578 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml remove 2,577,588 + -0.0 2,577,589 - -0.0 2,577,841 + RR42_RS12025 0.51 -0.4 2,577,851 + RR42_RS12025 0.53 -1.5 2,577,851 + RR42_RS12025 0.53 -1.8 2,577,852 - RR42_RS12025 0.53 -0.9 2,577,903 + RR42_RS12025 0.62 -0.2 2,577,903 + RR42_RS12025 0.62 +1.3 2,577,903 + RR42_RS12025 0.62 +0.5 2,577,903 + RR42_RS12025 0.62 -0.2 2,577,903 + RR42_RS12025 0.62 -0.7 2,577,903 + RR42_RS12025 0.62 +0.8 2,577,903 + RR42_RS12025 0.62 -0.0 2,577,904 - RR42_RS12025 0.62 +1.0 2,577,904 - RR42_RS12025 0.62 +0.4 2,577,904 - RR42_RS12025 0.62 +1.4 2,577,904 - RR42_RS12025 0.62 +0.3 2,577,961 - RR42_RS12025 0.72 +0.8 2,577,961 - RR42_RS12025 0.72 -1.2 2,577,987 + RR42_RS12025 0.76 +2.0 2,577,988 - RR42_RS12025 0.76 -0.3 2,577,988 - RR42_RS12025 0.76 -0.1 2,578,162 - -0.6 2,579,212 + RR42_RS12030 0.74 -0.8 2,579,543 + -1.4 2,579,543 + -0.8 2,579,543 + -2.3 2,579,543 + -2.4 2,579,543 + -1.3 2,579,544 - -2.7 2,579,544 - -1.7 2,579,545 + -2.6 2,579,545 + -2.0 2,579,545 + -2.9 2,579,546 - -1.9 2,579,546 - -3.4 2,579,546 - -2.2 2,579,546 - -1.8 2,579,562 - -1.7 2,579,577 + -1.6 2,579,578 - -2.7
Or see this region's nucleotide sequence