Experiment: R2A with Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11220 and RR42_RS11225 are separated by 41 nucleotides RR42_RS11225 and RR42_RS11230 are separated by 14 nucleotides RR42_RS11230 and RR42_RS11235 overlap by 4 nucleotides
RR42_RS11220: RR42_RS11220 - peptide ABC transporter, at 2,397,743 to 2,398,678
_RS11220
RR42_RS11225: RR42_RS11225 - peptide ABC transporter permease, at 2,398,720 to 2,399,622
_RS11225
RR42_RS11230: RR42_RS11230 - peptide ABC transporter ATP-binding protein, at 2,399,637 to 2,400,680
_RS11230
RR42_RS11235: RR42_RS11235 - peptide ABC transporter substrate-binding protein, at 2,400,677 to 2,401,669
_RS11235
Position (kb)
2399
2400
2401 Strain fitness (log2 ratio)
-1
0
1
2 at 2398.673 kb on + strand at 2398.674 kb on - strand at 2399.287 kb on + strand, within RR42_RS11225 at 2399.287 kb on + strand, within RR42_RS11225 at 2399.514 kb on + strand, within RR42_RS11225 at 2399.515 kb on - strand at 2399.515 kb on - strand, within RR42_RS11225 at 2399.758 kb on - strand, within RR42_RS11230 at 2400.283 kb on + strand, within RR42_RS11230 at 2400.283 kb on + strand, within RR42_RS11230 at 2400.284 kb on - strand, within RR42_RS11230 at 2400.357 kb on + strand, within RR42_RS11230 at 2400.494 kb on - strand, within RR42_RS11230 at 2400.738 kb on - strand at 2401.001 kb on + strand, within RR42_RS11235 at 2401.357 kb on + strand, within RR42_RS11235 at 2401.382 kb on + strand, within RR42_RS11235 at 2401.383 kb on - strand, within RR42_RS11235 at 2401.446 kb on - strand, within RR42_RS11235 at 2401.590 kb on + strand at 2401.591 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml remove 2,398,673 + -0.0 2,398,674 - -0.0 2,399,287 + RR42_RS11225 0.63 -0.2 2,399,287 + RR42_RS11225 0.63 +1.7 2,399,514 + RR42_RS11225 0.88 +0.7 2,399,515 - +0.3 2,399,515 - RR42_RS11225 0.88 +0.0 2,399,758 - RR42_RS11230 0.12 -0.8 2,400,283 + RR42_RS11230 0.62 -0.3 2,400,283 + RR42_RS11230 0.62 -0.5 2,400,284 - RR42_RS11230 0.62 +1.9 2,400,357 + RR42_RS11230 0.69 -1.0 2,400,494 - RR42_RS11230 0.82 -0.9 2,400,738 - -1.4 2,401,001 + RR42_RS11235 0.33 -0.6 2,401,357 + RR42_RS11235 0.68 -0.5 2,401,382 + RR42_RS11235 0.71 +0.0 2,401,383 - RR42_RS11235 0.71 -0.9 2,401,446 - RR42_RS11235 0.77 -1.4 2,401,590 + -1.0 2,401,591 - +0.3
Or see this region's nucleotide sequence