Experiment: R2A with Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS08850 and RR42_RS08855 are separated by 65 nucleotides RR42_RS08855 and RR42_RS08860 are separated by 43 nucleotides RR42_RS08860 and RR42_RS08865 are separated by 190 nucleotides RR42_RS08865 and RR42_RS08870 are separated by 50 nucleotides
RR42_RS08850: RR42_RS08850 - ribonucleoside-triphosphate reductase, at 1,859,199 to 1,860,941
_RS08850
RR42_RS08855: RR42_RS08855 - hypothetical protein, at 1,861,007 to 1,861,207
_RS08855
RR42_RS08860: RR42_RS08860 - radical SAM protein, at 1,861,251 to 1,861,910
_RS08860
RR42_RS08865: RR42_RS08865 - hydroxylase, at 1,862,101 to 1,862,388
_RS08865
RR42_RS08870: RR42_RS08870 - hypothetical protein, at 1,862,439 to 1,863,524
_RS08870
Position (kb)
1861
1862 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1860.325 kb on - strand, within RR42_RS08850 at 1860.325 kb on - strand, within RR42_RS08850 at 1860.325 kb on - strand, within RR42_RS08850 at 1860.336 kb on + strand, within RR42_RS08850 at 1860.336 kb on + strand, within RR42_RS08850 at 1860.336 kb on + strand, within RR42_RS08850 at 1860.519 kb on + strand, within RR42_RS08850 at 1860.625 kb on - strand, within RR42_RS08850 at 1860.627 kb on + strand, within RR42_RS08850 at 1860.628 kb on - strand, within RR42_RS08850 at 1860.738 kb on + strand, within RR42_RS08850 at 1860.739 kb on - strand, within RR42_RS08850 at 1860.739 kb on - strand, within RR42_RS08850 at 1860.816 kb on + strand at 1860.817 kb on - strand at 1860.817 kb on - strand at 1860.999 kb on + strand at 1861.115 kb on + strand, within RR42_RS08855 at 1861.116 kb on - strand, within RR42_RS08855 at 1861.210 kb on + strand at 1861.210 kb on + strand at 1861.211 kb on - strand at 1861.347 kb on + strand, within RR42_RS08860 at 1861.348 kb on - strand, within RR42_RS08860 at 1861.348 kb on - strand, within RR42_RS08860 at 1861.554 kb on + strand, within RR42_RS08860 at 1861.854 kb on + strand at 1861.931 kb on + strand at 1861.931 kb on + strand at 1862.015 kb on + strand at 1862.016 kb on - strand at 1862.016 kb on - strand at 1862.016 kb on - strand at 1862.177 kb on - strand, within RR42_RS08865 at 1862.177 kb on - strand, within RR42_RS08865 at 1862.321 kb on + strand, within RR42_RS08865 at 1862.532 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml remove 1,860,325 - RR42_RS08850 0.65 +0.5 1,860,325 - RR42_RS08850 0.65 -0.1 1,860,325 - RR42_RS08850 0.65 +1.4 1,860,336 + RR42_RS08850 0.65 -0.8 1,860,336 + RR42_RS08850 0.65 -0.7 1,860,336 + RR42_RS08850 0.65 -0.7 1,860,519 + RR42_RS08850 0.76 -0.4 1,860,625 - RR42_RS08850 0.82 -1.1 1,860,627 + RR42_RS08850 0.82 -0.1 1,860,628 - RR42_RS08850 0.82 +0.1 1,860,738 + RR42_RS08850 0.88 +0.6 1,860,739 - RR42_RS08850 0.88 -1.5 1,860,739 - RR42_RS08850 0.88 +1.2 1,860,816 + -0.1 1,860,817 - +0.1 1,860,817 - -0.6 1,860,999 + +1.9 1,861,115 + RR42_RS08855 0.54 +0.3 1,861,116 - RR42_RS08855 0.54 -2.1 1,861,210 + +0.1 1,861,210 + -0.5 1,861,211 - +0.2 1,861,347 + RR42_RS08860 0.15 +0.7 1,861,348 - RR42_RS08860 0.15 +1.3 1,861,348 - RR42_RS08860 0.15 +1.2 1,861,554 + RR42_RS08860 0.46 +0.5 1,861,854 + -0.5 1,861,931 + +0.2 1,861,931 + -0.1 1,862,015 + -0.9 1,862,016 - -0.5 1,862,016 - +1.1 1,862,016 - -0.8 1,862,177 - RR42_RS08865 0.26 +0.7 1,862,177 - RR42_RS08865 0.26 -1.6 1,862,321 + RR42_RS08865 0.76 -0.1 1,862,532 + +0.9
Or see this region's nucleotide sequence