Experiment: R2A with Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS08580 and RR42_RS08585 overlap by 4 nucleotides RR42_RS08585 and RR42_RS08590 are separated by 14 nucleotides RR42_RS08590 and RR42_RS08595 are separated by 281 nucleotides RR42_RS08595 and RR42_RS08600 overlap by 4 nucleotides
RR42_RS08580: RR42_RS08580 - baseplate assembly protein, at 1,803,458 to 1,804,366
_RS08580
RR42_RS08585: RR42_RS08585 - baseplate assembly protein, at 1,804,363 to 1,804,707
_RS08585
RR42_RS08590: RR42_RS08590 - baseplate assembly protein, at 1,804,722 to 1,805,339
_RS08590
RR42_RS08595: RR42_RS08595 - virion morphogenesis protein, at 1,805,621 to 1,806,067
_RS08595
RR42_RS08600: RR42_RS08600 - tail protein, at 1,806,064 to 1,806,504
_RS08600
Position (kb) 1804
1805
1806 Strain fitness (log2 ratio) -1
0
1 at 1803.936 kb on + strand, within RR42_RS08580 at 1803.937 kb on - strand, within RR42_RS08580 at 1803.937 kb on - strand, within RR42_RS08580 at 1804.017 kb on - strand, within RR42_RS08580 at 1804.445 kb on - strand, within RR42_RS08585 at 1804.448 kb on + strand, within RR42_RS08585 at 1804.490 kb on - strand, within RR42_RS08585 at 1804.602 kb on - strand, within RR42_RS08585 at 1804.804 kb on - strand, within RR42_RS08590 at 1804.962 kb on + strand, within RR42_RS08590 at 1805.412 kb on + strand at 1805.412 kb on + strand at 1805.493 kb on + strand at 1805.494 kb on - strand at 1805.594 kb on + strand at 1805.611 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml remove 1,803,936 + RR42_RS08580 0.53 +0.3 1,803,937 - RR42_RS08580 0.53 +0.0 1,803,937 - RR42_RS08580 0.53 -0.4 1,804,017 - RR42_RS08580 0.61 -1.1 1,804,445 - RR42_RS08585 0.24 -0.4 1,804,448 + RR42_RS08585 0.25 -0.4 1,804,490 - RR42_RS08585 0.37 -0.7 1,804,602 - RR42_RS08585 0.69 +0.2 1,804,804 - RR42_RS08590 0.13 +0.3 1,804,962 + RR42_RS08590 0.39 -0.4 1,805,412 + -0.1 1,805,412 + -0.1 1,805,493 + +0.6 1,805,494 - -0.3 1,805,594 + +1.1 1,805,611 + -0.6
Or see this region's nucleotide sequence