Experiment: R2A with Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS07645 and RR42_RS07650 are separated by 49 nucleotides RR42_RS07650 and RR42_RS07655 are separated by 155 nucleotides RR42_RS07655 and RR42_RS07660 are separated by 53 nucleotides
RR42_RS07645: RR42_RS07645 - acetyl-CoA acetyltransferase, at 1,637,768 to 1,638,952
_RS07645
RR42_RS07650: RR42_RS07650 - hypothetical protein, at 1,639,002 to 1,639,649
_RS07650
RR42_RS07655: RR42_RS07655 - cystathionine beta-lyase, at 1,639,805 to 1,640,983
_RS07655
RR42_RS07660: RR42_RS07660 - phosphoserine phosphatase, at 1,641,037 to 1,641,891
_RS07660
Position (kb)
1639
1640
1641 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1638.887 kb on + strand at 1638.997 kb on + strand at 1638.997 kb on + strand at 1638.998 kb on - strand at 1638.998 kb on - strand at 1639.153 kb on + strand, within RR42_RS07650 at 1639.153 kb on + strand, within RR42_RS07650 at 1639.154 kb on - strand, within RR42_RS07650 at 1639.154 kb on - strand, within RR42_RS07650 at 1639.257 kb on + strand, within RR42_RS07650 at 1639.258 kb on - strand, within RR42_RS07650 at 1639.258 kb on - strand, within RR42_RS07650 at 1639.258 kb on - strand, within RR42_RS07650 at 1639.275 kb on + strand, within RR42_RS07650 at 1639.275 kb on + strand, within RR42_RS07650 at 1639.275 kb on + strand, within RR42_RS07650 at 1639.275 kb on + strand, within RR42_RS07650 at 1639.356 kb on + strand, within RR42_RS07650 at 1639.357 kb on - strand, within RR42_RS07650 at 1639.357 kb on - strand, within RR42_RS07650 at 1639.604 kb on + strand at 1639.605 kb on - strand at 1639.605 kb on - strand at 1639.609 kb on - strand at 1639.609 kb on - strand at 1639.609 kb on - strand at 1639.714 kb on - strand at 1639.870 kb on + strand at 1639.871 kb on - strand at 1639.871 kb on - strand at 1639.943 kb on + strand, within RR42_RS07655 at 1639.944 kb on - strand, within RR42_RS07655 at 1639.944 kb on - strand, within RR42_RS07655 at 1639.944 kb on - strand, within RR42_RS07655 at 1639.952 kb on + strand, within RR42_RS07655 at 1639.953 kb on - strand, within RR42_RS07655 at 1639.966 kb on + strand, within RR42_RS07655 at 1639.967 kb on - strand, within RR42_RS07655 at 1640.067 kb on - strand, within RR42_RS07655 at 1640.117 kb on + strand, within RR42_RS07655 at 1640.177 kb on + strand, within RR42_RS07655 at 1640.180 kb on + strand, within RR42_RS07655 at 1640.181 kb on - strand, within RR42_RS07655 at 1640.181 kb on - strand, within RR42_RS07655 at 1640.181 kb on - strand, within RR42_RS07655 at 1640.181 kb on - strand, within RR42_RS07655 at 1640.335 kb on + strand, within RR42_RS07655 at 1640.336 kb on - strand, within RR42_RS07655 at 1640.357 kb on + strand, within RR42_RS07655 at 1640.412 kb on - strand, within RR42_RS07655 at 1640.412 kb on - strand, within RR42_RS07655 at 1640.412 kb on - strand, within RR42_RS07655 at 1640.412 kb on - strand, within RR42_RS07655 at 1640.433 kb on + strand, within RR42_RS07655 at 1640.433 kb on + strand, within RR42_RS07655 at 1640.433 kb on + strand, within RR42_RS07655 at 1640.531 kb on + strand, within RR42_RS07655 at 1640.739 kb on - strand, within RR42_RS07655 at 1640.739 kb on - strand, within RR42_RS07655 at 1640.816 kb on - strand, within RR42_RS07655 at 1640.909 kb on + strand at 1640.910 kb on - strand at 1640.910 kb on - strand at 1640.910 kb on - strand at 1641.970 kb on - strand at 1641.974 kb on + strand at 1641.974 kb on + strand at 1641.974 kb on + strand at 1641.974 kb on + strand at 1641.974 kb on + strand at 1641.975 kb on - strand at 1641.975 kb on - strand at 1641.975 kb on - strand at 1641.975 kb on - strand at 1641.975 kb on - strand at 1641.975 kb on - strand at 1641.975 kb on - strand at 1641.975 kb on - strand at 1641.975 kb on - strand at 1641.975 kb on - strand at 1641.975 kb on - strand at 1641.976 kb on + strand at 1641.976 kb on + strand at 1641.977 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml remove 1,638,887 + +0.1 1,638,997 + -1.8 1,638,997 + -0.1 1,638,998 - +0.5 1,638,998 - -0.2 1,639,153 + RR42_RS07650 0.23 -0.4 1,639,153 + RR42_RS07650 0.23 +1.1 1,639,154 - RR42_RS07650 0.23 +0.4 1,639,154 - RR42_RS07650 0.23 +0.3 1,639,257 + RR42_RS07650 0.39 +2.1 1,639,258 - RR42_RS07650 0.40 +1.5 1,639,258 - RR42_RS07650 0.40 +0.0 1,639,258 - RR42_RS07650 0.40 -0.3 1,639,275 + RR42_RS07650 0.42 -0.5 1,639,275 + RR42_RS07650 0.42 -0.7 1,639,275 + RR42_RS07650 0.42 +1.0 1,639,275 + RR42_RS07650 0.42 -0.1 1,639,356 + RR42_RS07650 0.55 -0.5 1,639,357 - RR42_RS07650 0.55 -1.6 1,639,357 - RR42_RS07650 0.55 -0.5 1,639,604 + -0.1 1,639,605 - -0.2 1,639,605 - +0.7 1,639,609 - +0.1 1,639,609 - -0.3 1,639,609 - -0.1 1,639,714 - +1.7 1,639,870 + -0.5 1,639,871 - +0.8 1,639,871 - +0.1 1,639,943 + RR42_RS07655 0.12 +0.1 1,639,944 - RR42_RS07655 0.12 -0.1 1,639,944 - RR42_RS07655 0.12 +0.1 1,639,944 - RR42_RS07655 0.12 +1.4 1,639,952 + RR42_RS07655 0.12 +0.0 1,639,953 - RR42_RS07655 0.13 -0.3 1,639,966 + RR42_RS07655 0.14 -0.1 1,639,967 - RR42_RS07655 0.14 +0.5 1,640,067 - RR42_RS07655 0.22 -0.3 1,640,117 + RR42_RS07655 0.26 +0.1 1,640,177 + RR42_RS07655 0.32 -0.3 1,640,180 + RR42_RS07655 0.32 -0.2 1,640,181 - RR42_RS07655 0.32 +0.5 1,640,181 - RR42_RS07655 0.32 +0.5 1,640,181 - RR42_RS07655 0.32 -0.8 1,640,181 - RR42_RS07655 0.32 -0.6 1,640,335 + RR42_RS07655 0.45 -1.6 1,640,336 - RR42_RS07655 0.45 -0.1 1,640,357 + RR42_RS07655 0.47 -0.5 1,640,412 - RR42_RS07655 0.51 +0.3 1,640,412 - RR42_RS07655 0.51 +0.3 1,640,412 - RR42_RS07655 0.51 +0.3 1,640,412 - RR42_RS07655 0.51 -2.6 1,640,433 + RR42_RS07655 0.53 +0.7 1,640,433 + RR42_RS07655 0.53 +0.3 1,640,433 + RR42_RS07655 0.53 -0.6 1,640,531 + RR42_RS07655 0.62 -1.4 1,640,739 - RR42_RS07655 0.79 +0.7 1,640,739 - RR42_RS07655 0.79 +0.7 1,640,816 - RR42_RS07655 0.86 +0.5 1,640,909 + +0.7 1,640,910 - -1.3 1,640,910 - +1.0 1,640,910 - +1.0 1,641,970 - -0.2 1,641,974 + +0.3 1,641,974 + +1.1 1,641,974 + -0.9 1,641,974 + -0.3 1,641,974 + -0.6 1,641,975 - -0.1 1,641,975 - +2.7 1,641,975 - -1.1 1,641,975 - -0.1 1,641,975 - -2.0 1,641,975 - +0.6 1,641,975 - +0.2 1,641,975 - +0.1 1,641,975 - +0.2 1,641,975 - -0.3 1,641,975 - -0.3 1,641,976 + +0.4 1,641,976 + -1.3 1,641,977 - -0.5
Or see this region's nucleotide sequence