Experiment: R2A with Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06335 and RR42_RS06340 are separated by 32 nucleotides RR42_RS06340 and RR42_RS06345 are separated by 70 nucleotides RR42_RS06345 and RR42_RS06350 are separated by 254 nucleotides
RR42_RS06335: RR42_RS06335 - cyclophilin, at 1,390,115 to 1,390,615
_RS06335
RR42_RS06340: RR42_RS06340 - peptidyl-prolyl cis-trans isomerase, at 1,390,648 to 1,391,232
_RS06340
RR42_RS06345: RR42_RS06345 - hypothetical protein, at 1,391,303 to 1,392,001
_RS06345
RR42_RS06350: RR42_RS06350 - cysteinyl-tRNA synthetase, at 1,392,256 to 1,393,644
_RS06350
Position (kb)
1391
1392
1393 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1390.522 kb on - strand, within RR42_RS06335 at 1390.522 kb on - strand, within RR42_RS06335 at 1390.605 kb on - strand at 1390.605 kb on - strand at 1390.699 kb on - strand at 1390.713 kb on - strand, within RR42_RS06340 at 1390.825 kb on - strand, within RR42_RS06340 at 1391.028 kb on + strand, within RR42_RS06340 at 1391.029 kb on - strand, within RR42_RS06340 at 1391.030 kb on + strand, within RR42_RS06340 at 1391.030 kb on + strand, within RR42_RS06340 at 1391.031 kb on - strand, within RR42_RS06340 at 1391.031 kb on - strand, within RR42_RS06340 at 1391.031 kb on - strand, within RR42_RS06340 at 1391.031 kb on - strand, within RR42_RS06340 at 1391.031 kb on - strand, within RR42_RS06340 at 1391.048 kb on + strand, within RR42_RS06340 at 1391.048 kb on + strand, within RR42_RS06340 at 1391.212 kb on + strand at 1391.212 kb on + strand at 1391.213 kb on - strand at 1391.213 kb on - strand at 1391.300 kb on + strand at 1391.301 kb on - strand at 1391.301 kb on - strand at 1391.304 kb on + strand at 1391.304 kb on + strand at 1391.304 kb on + strand at 1391.304 kb on + strand at 1391.305 kb on - strand at 1391.454 kb on + strand, within RR42_RS06345 at 1391.454 kb on + strand, within RR42_RS06345 at 1391.500 kb on - strand, within RR42_RS06345 at 1391.581 kb on - strand, within RR42_RS06345 at 1391.581 kb on - strand, within RR42_RS06345 at 1391.767 kb on - strand, within RR42_RS06345 at 1391.979 kb on - strand at 1392.003 kb on + strand at 1392.003 kb on + strand at 1392.004 kb on - strand at 1392.004 kb on - strand at 1392.020 kb on - strand at 1392.035 kb on - strand at 1392.163 kb on + strand at 1392.163 kb on + strand at 1392.163 kb on + strand at 1392.163 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml remove 1,390,522 - RR42_RS06335 0.81 -0.6 1,390,522 - RR42_RS06335 0.81 +1.9 1,390,605 - -1.8 1,390,605 - -0.0 1,390,699 - +0.2 1,390,713 - RR42_RS06340 0.11 -0.2 1,390,825 - RR42_RS06340 0.30 -0.2 1,391,028 + RR42_RS06340 0.65 +1.5 1,391,029 - RR42_RS06340 0.65 +1.1 1,391,030 + RR42_RS06340 0.65 -0.2 1,391,030 + RR42_RS06340 0.65 -1.3 1,391,031 - RR42_RS06340 0.65 -1.3 1,391,031 - RR42_RS06340 0.65 -0.4 1,391,031 - RR42_RS06340 0.65 +0.3 1,391,031 - RR42_RS06340 0.65 +0.3 1,391,031 - RR42_RS06340 0.65 +0.1 1,391,048 + RR42_RS06340 0.68 +0.2 1,391,048 + RR42_RS06340 0.68 -0.0 1,391,212 + +2.0 1,391,212 + +2.3 1,391,213 - -1.5 1,391,213 - +0.6 1,391,300 + +0.1 1,391,301 - -0.3 1,391,301 - +0.4 1,391,304 + +0.4 1,391,304 + -0.7 1,391,304 + -0.5 1,391,304 + -1.3 1,391,305 - +0.2 1,391,454 + RR42_RS06345 0.22 -0.1 1,391,454 + RR42_RS06345 0.22 -1.1 1,391,500 - RR42_RS06345 0.28 -0.5 1,391,581 - RR42_RS06345 0.40 -1.0 1,391,581 - RR42_RS06345 0.40 +1.2 1,391,767 - RR42_RS06345 0.66 +0.2 1,391,979 - +1.7 1,392,003 + -2.2 1,392,003 + +0.7 1,392,004 - -0.0 1,392,004 - +0.1 1,392,020 - +0.7 1,392,035 - -0.7 1,392,163 + +0.3 1,392,163 + +0.2 1,392,163 + -0.0 1,392,163 + -0.6
Or see this region's nucleotide sequence