Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS03025

Experiment: R2A with Cisplatin 0.001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS03015 and RR42_RS03020 are separated by 354 nucleotidesRR42_RS03020 and RR42_RS03025 are separated by 4 nucleotidesRR42_RS03025 and RR42_RS03030 are separated by 89 nucleotidesRR42_RS03030 and RR42_RS03035 overlap by 4 nucleotides RR42_RS03015: RR42_RS03015 - enoyl-CoA hydratase, at 667,753 to 668,550 _RS03015 RR42_RS03020: RR42_RS03020 - MarR family transcriptional regulator, at 668,905 to 669,366 _RS03020 RR42_RS03025: RR42_RS03025 - Inner membrane protein (FUSC-like) required for 4-hydroxybenzoate transport, together with NodT, MFP, and DUF1656 proteins (RR42_RS03040, RR42_RS03035, and RR42_RS03030) (from data), at 669,371 to 671,365 _RS03025 RR42_RS03030: RR42_RS03030 - required for 4-hydroxybenzoate transport, together with NodT, MFP, and FUSC proteins (RR42_RS03040, RR42_RS03035, and RR42_RS03025) (from data), at 671,455 to 671,664 _RS03030 RR42_RS03035: RR42_RS03035 - membrane protein, at 671,661 to 672,629 _RS03035 Position (kb) 669 670 671 672Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 668.729 kb on - strandat 668.730 kb on + strandat 668.731 kb on - strandat 668.838 kb on - strandat 669.085 kb on + strand, within RR42_RS03020at 669.086 kb on - strand, within RR42_RS03020at 669.364 kb on + strandat 669.364 kb on + strandat 669.455 kb on + strandat 669.497 kb on + strandat 669.497 kb on + strandat 669.498 kb on - strandat 669.498 kb on - strandat 669.498 kb on - strandat 669.500 kb on - strandat 669.500 kb on - strandat 669.500 kb on - strandat 669.695 kb on + strand, within RR42_RS03025at 669.695 kb on + strand, within RR42_RS03025at 669.695 kb on + strand, within RR42_RS03025at 669.696 kb on - strand, within RR42_RS03025at 669.696 kb on - strand, within RR42_RS03025at 669.696 kb on - strand, within RR42_RS03025at 669.696 kb on - strand, within RR42_RS03025at 669.696 kb on - strand, within RR42_RS03025at 669.716 kb on + strand, within RR42_RS03025at 669.717 kb on - strand, within RR42_RS03025at 669.718 kb on + strand, within RR42_RS03025at 669.718 kb on + strand, within RR42_RS03025at 669.718 kb on + strand, within RR42_RS03025at 669.718 kb on + strand, within RR42_RS03025at 669.719 kb on - strand, within RR42_RS03025at 669.739 kb on - strand, within RR42_RS03025at 669.918 kb on - strand, within RR42_RS03025at 670.023 kb on - strand, within RR42_RS03025at 670.111 kb on + strand, within RR42_RS03025at 670.452 kb on - strand, within RR42_RS03025at 670.668 kb on - strand, within RR42_RS03025at 670.668 kb on - strand, within RR42_RS03025at 670.714 kb on - strand, within RR42_RS03025at 670.714 kb on - strand, within RR42_RS03025at 670.733 kb on + strand, within RR42_RS03025at 670.833 kb on - strand, within RR42_RS03025at 671.213 kb on + strandat 671.216 kb on + strandat 671.217 kb on - strandat 671.217 kb on - strandat 671.217 kb on - strandat 671.476 kb on + strand, within RR42_RS03030at 671.476 kb on + strand, within RR42_RS03030at 671.476 kb on + strand, within RR42_RS03030at 671.476 kb on + strand, within RR42_RS03030at 671.477 kb on - strand, within RR42_RS03030at 671.477 kb on - strand, within RR42_RS03030at 671.609 kb on + strand, within RR42_RS03030at 671.609 kb on + strand, within RR42_RS03030at 671.610 kb on - strand, within RR42_RS03030at 671.610 kb on - strand, within RR42_RS03030at 671.610 kb on - strand, within RR42_RS03030at 671.610 kb on - strand, within RR42_RS03030at 671.611 kb on + strand, within RR42_RS03030at 671.611 kb on + strand, within RR42_RS03030at 671.611 kb on + strand, within RR42_RS03030at 671.611 kb on + strand, within RR42_RS03030at 671.611 kb on + strand, within RR42_RS03030at 671.612 kb on - strand, within RR42_RS03030at 671.612 kb on - strand, within RR42_RS03030at 671.639 kb on - strand, within RR42_RS03030at 671.639 kb on - strand, within RR42_RS03030at 671.678 kb on + strandat 671.699 kb on + strandat 671.752 kb on - strandat 671.754 kb on + strandat 671.754 kb on + strandat 672.147 kb on + strand, within RR42_RS03035

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml
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668,729 - +2.0
668,730 + -0.7
668,731 - -0.0
668,838 - -1.3
669,085 + RR42_RS03020 0.39 +0.1
669,086 - RR42_RS03020 0.39 +0.1
669,364 + -1.0
669,364 + +0.3
669,455 + +0.1
669,497 + -1.2
669,497 + -0.8
669,498 - +0.4
669,498 - +0.9
669,498 - +1.6
669,500 - +0.5
669,500 - +0.1
669,500 - +0.6
669,695 + RR42_RS03025 0.16 -0.2
669,695 + RR42_RS03025 0.16 +0.7
669,695 + RR42_RS03025 0.16 +0.8
669,696 - RR42_RS03025 0.16 +0.2
669,696 - RR42_RS03025 0.16 -0.2
669,696 - RR42_RS03025 0.16 -0.0
669,696 - RR42_RS03025 0.16 +1.4
669,696 - RR42_RS03025 0.16 +0.9
669,716 + RR42_RS03025 0.17 -0.9
669,717 - RR42_RS03025 0.17 +1.9
669,718 + RR42_RS03025 0.17 +0.8
669,718 + RR42_RS03025 0.17 -0.4
669,718 + RR42_RS03025 0.17 +0.1
669,718 + RR42_RS03025 0.17 +0.1
669,719 - RR42_RS03025 0.17 +0.4
669,739 - RR42_RS03025 0.18 +1.1
669,918 - RR42_RS03025 0.27 +0.1
670,023 - RR42_RS03025 0.33 -2.1
670,111 + RR42_RS03025 0.37 -0.7
670,452 - RR42_RS03025 0.54 +0.7
670,668 - RR42_RS03025 0.65 -1.1
670,668 - RR42_RS03025 0.65 +0.3
670,714 - RR42_RS03025 0.67 -0.0
670,714 - RR42_RS03025 0.67 +0.1
670,733 + RR42_RS03025 0.68 +1.8
670,833 - RR42_RS03025 0.73 +1.5
671,213 + -0.5
671,216 + +1.6
671,217 - -0.9
671,217 - +1.1
671,217 - +1.4
671,476 + RR42_RS03030 0.10 -0.0
671,476 + RR42_RS03030 0.10 -0.7
671,476 + RR42_RS03030 0.10 -0.0
671,476 + RR42_RS03030 0.10 +2.1
671,477 - RR42_RS03030 0.10 -0.1
671,477 - RR42_RS03030 0.10 -1.6
671,609 + RR42_RS03030 0.73 +0.3
671,609 + RR42_RS03030 0.73 +0.1
671,610 - RR42_RS03030 0.74 -0.5
671,610 - RR42_RS03030 0.74 -1.1
671,610 - RR42_RS03030 0.74 +0.4
671,610 - RR42_RS03030 0.74 +0.3
671,611 + RR42_RS03030 0.74 +1.5
671,611 + RR42_RS03030 0.74 +0.5
671,611 + RR42_RS03030 0.74 +0.6
671,611 + RR42_RS03030 0.74 -0.4
671,611 + RR42_RS03030 0.74 +0.0
671,612 - RR42_RS03030 0.75 -0.4
671,612 - RR42_RS03030 0.75 -1.3
671,639 - RR42_RS03030 0.88 -0.4
671,639 - RR42_RS03030 0.88 +0.8
671,678 + -0.1
671,699 + -0.1
671,752 - -1.1
671,754 + +0.7
671,754 + +0.3
672,147 + RR42_RS03035 0.50 -2.8

Or see this region's nucleotide sequence