Experiment: R2A with Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01410 and RR42_RS01415 are separated by 200 nucleotides RR42_RS01415 and RR42_RS01420 are separated by 8 nucleotides
RR42_RS01410: RR42_RS01410 - NAD-dependent dehydratase, at 318,260 to 319,285
_RS01410
RR42_RS01415: RR42_RS01415 - glutathione S-transferase, at 319,486 to 320,175
_RS01415
RR42_RS01420: RR42_RS01420 - 2', 3'-cyclic nucleotide 2'-phosphodiesterase, at 320,184 to 321,419
_RS01420
Position (kb) 319
320
321 Strain fitness (log2 ratio) -2
-1
0
1
2 at 318.714 kb on - strand, within RR42_RS01410 at 318.714 kb on - strand, within RR42_RS01410 at 318.893 kb on + strand, within RR42_RS01410 at 318.893 kb on + strand, within RR42_RS01410 at 318.893 kb on + strand, within RR42_RS01410 at 318.894 kb on - strand, within RR42_RS01410 at 318.894 kb on - strand, within RR42_RS01410 at 318.959 kb on + strand, within RR42_RS01410 at 318.983 kb on + strand, within RR42_RS01410 at 318.984 kb on - strand, within RR42_RS01410 at 318.984 kb on - strand, within RR42_RS01410 at 318.985 kb on + strand, within RR42_RS01410 at 318.985 kb on + strand, within RR42_RS01410 at 318.985 kb on + strand, within RR42_RS01410 at 318.985 kb on + strand, within RR42_RS01410 at 318.986 kb on - strand, within RR42_RS01410 at 318.986 kb on - strand, within RR42_RS01410 at 318.986 kb on - strand, within RR42_RS01410 at 319.351 kb on - strand at 319.351 kb on - strand at 319.382 kb on + strand at 319.382 kb on + strand at 319.383 kb on - strand at 319.513 kb on + strand at 319.513 kb on + strand at 319.513 kb on + strand at 319.643 kb on + strand, within RR42_RS01415 at 319.643 kb on + strand, within RR42_RS01415 at 319.644 kb on - strand, within RR42_RS01415 at 319.699 kb on + strand, within RR42_RS01415 at 320.005 kb on + strand, within RR42_RS01415 at 320.005 kb on + strand, within RR42_RS01415 at 320.005 kb on + strand, within RR42_RS01415 at 320.005 kb on + strand, within RR42_RS01415 at 320.006 kb on - strand, within RR42_RS01415 at 320.038 kb on + strand, within RR42_RS01415 at 320.193 kb on + strand at 320.193 kb on + strand at 320.859 kb on + strand, within RR42_RS01420 at 321.005 kb on + strand, within RR42_RS01420
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml remove 318,714 - RR42_RS01410 0.44 +0.1 318,714 - RR42_RS01410 0.44 -1.2 318,893 + RR42_RS01410 0.62 -0.2 318,893 + RR42_RS01410 0.62 +0.4 318,893 + RR42_RS01410 0.62 +0.3 318,894 - RR42_RS01410 0.62 +0.3 318,894 - RR42_RS01410 0.62 -0.1 318,959 + RR42_RS01410 0.68 -0.9 318,983 + RR42_RS01410 0.70 +1.9 318,984 - RR42_RS01410 0.71 -0.1 318,984 - RR42_RS01410 0.71 -0.2 318,985 + RR42_RS01410 0.71 -1.2 318,985 + RR42_RS01410 0.71 -2.2 318,985 + RR42_RS01410 0.71 -2.3 318,985 + RR42_RS01410 0.71 +0.8 318,986 - RR42_RS01410 0.71 -0.8 318,986 - RR42_RS01410 0.71 +0.6 318,986 - RR42_RS01410 0.71 -0.2 319,351 - -0.4 319,351 - -1.5 319,382 + +2.1 319,382 + +0.3 319,383 - +0.7 319,513 + +0.1 319,513 + +0.2 319,513 + +0.1 319,643 + RR42_RS01415 0.23 +0.1 319,643 + RR42_RS01415 0.23 -0.7 319,644 - RR42_RS01415 0.23 -0.3 319,699 + RR42_RS01415 0.31 -1.9 320,005 + RR42_RS01415 0.75 -2.1 320,005 + RR42_RS01415 0.75 -1.7 320,005 + RR42_RS01415 0.75 +0.6 320,005 + RR42_RS01415 0.75 -0.2 320,006 - RR42_RS01415 0.75 -0.7 320,038 + RR42_RS01415 0.80 -0.0 320,193 + +0.7 320,193 + +1.6 320,859 + RR42_RS01420 0.55 +0.3 321,005 + RR42_RS01420 0.66 +1.1
Or see this region's nucleotide sequence