Experiment: R2A with Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01210 and RR42_RS01215 are separated by 78 nucleotides RR42_RS01215 and RR42_RS01220 are separated by 30 nucleotides RR42_RS01220 and RR42_RS01225 are separated by 30 nucleotides
RR42_RS01210: RR42_RS01210 - n-acetyl-gamma-glutamyl-phosphate reductase, at 275,725 to 276,672
_RS01210
RR42_RS01215: RR42_RS01215 - hypothetical protein, at 276,751 to 276,939
_RS01215
RR42_RS01220: RR42_RS01220 - exodeoxyribonuclease III, at 276,970 to 277,746
_RS01220
RR42_RS01225: RR42_RS01225 - orotate phosphoribosyltransferase, at 277,777 to 278,466
_RS01225
Position (kb) 276
277 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 275.966 kb on + strand, within RR42_RS01210 at 275.966 kb on + strand, within RR42_RS01210 at 275.967 kb on - strand, within RR42_RS01210 at 276.230 kb on + strand, within RR42_RS01210 at 276.399 kb on - strand, within RR42_RS01210 at 276.450 kb on + strand, within RR42_RS01210 at 276.451 kb on - strand, within RR42_RS01210 at 276.612 kb on - strand at 276.652 kb on + strand at 276.653 kb on - strand at 276.653 kb on - strand at 276.653 kb on - strand at 276.653 kb on - strand at 276.692 kb on - strand at 276.692 kb on - strand at 276.733 kb on + strand at 276.733 kb on + strand at 276.733 kb on + strand at 276.733 kb on + strand at 276.734 kb on - strand at 276.737 kb on + strand at 276.737 kb on + strand at 276.737 kb on + strand at 276.737 kb on + strand at 276.737 kb on + strand at 276.737 kb on + strand at 276.737 kb on + strand at 276.738 kb on - strand at 276.738 kb on - strand at 276.738 kb on - strand at 276.781 kb on - strand, within RR42_RS01215 at 276.784 kb on + strand, within RR42_RS01215 at 276.784 kb on + strand, within RR42_RS01215 at 276.785 kb on - strand, within RR42_RS01215 at 276.785 kb on - strand, within RR42_RS01215 at 276.785 kb on - strand, within RR42_RS01215 at 276.938 kb on - strand at 276.965 kb on - strand at 276.965 kb on - strand at 276.965 kb on - strand at 276.971 kb on + strand at 276.971 kb on + strand at 276.972 kb on - strand at 276.972 kb on - strand at 276.972 kb on - strand at 277.031 kb on + strand at 277.142 kb on + strand, within RR42_RS01220 at 277.142 kb on + strand, within RR42_RS01220 at 277.142 kb on + strand, within RR42_RS01220 at 277.142 kb on + strand, within RR42_RS01220 at 277.142 kb on + strand, within RR42_RS01220 at 277.143 kb on - strand, within RR42_RS01220 at 277.143 kb on - strand, within RR42_RS01220 at 277.143 kb on - strand, within RR42_RS01220 at 277.143 kb on - strand, within RR42_RS01220 at 277.143 kb on - strand, within RR42_RS01220 at 277.421 kb on + strand, within RR42_RS01220 at 277.421 kb on + strand, within RR42_RS01220 at 277.421 kb on + strand, within RR42_RS01220 at 277.422 kb on - strand, within RR42_RS01220 at 277.448 kb on + strand, within RR42_RS01220 at 277.463 kb on + strand, within RR42_RS01220 at 277.548 kb on + strand, within RR42_RS01220 at 277.548 kb on + strand, within RR42_RS01220 at 277.549 kb on - strand, within RR42_RS01220 at 277.568 kb on + strand, within RR42_RS01220 at 277.577 kb on + strand, within RR42_RS01220 at 277.577 kb on + strand, within RR42_RS01220 at 277.734 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml remove 275,966 + RR42_RS01210 0.25 -0.3 275,966 + RR42_RS01210 0.25 -1.2 275,967 - RR42_RS01210 0.26 -0.5 276,230 + RR42_RS01210 0.53 -1.3 276,399 - RR42_RS01210 0.71 -1.8 276,450 + RR42_RS01210 0.76 +0.5 276,451 - RR42_RS01210 0.77 -0.3 276,612 - -0.5 276,652 + +0.3 276,653 - -0.7 276,653 - +0.5 276,653 - +1.4 276,653 - -0.7 276,692 - +0.1 276,692 - +0.3 276,733 + +0.7 276,733 + +0.3 276,733 + +2.1 276,733 + +1.4 276,734 - +0.7 276,737 + +1.7 276,737 + +0.4 276,737 + -0.2 276,737 + -2.6 276,737 + -1.4 276,737 + +0.3 276,737 + -1.1 276,738 - -0.7 276,738 - -0.9 276,738 - -0.2 276,781 - RR42_RS01215 0.16 -0.7 276,784 + RR42_RS01215 0.17 -0.6 276,784 + RR42_RS01215 0.17 -0.7 276,785 - RR42_RS01215 0.18 -1.3 276,785 - RR42_RS01215 0.18 -1.2 276,785 - RR42_RS01215 0.18 -0.4 276,938 - +1.3 276,965 - -0.2 276,965 - +0.6 276,965 - -0.2 276,971 + +1.1 276,971 + +0.5 276,972 - +0.8 276,972 - +0.3 276,972 - -0.6 277,031 + +0.3 277,142 + RR42_RS01220 0.22 +0.8 277,142 + RR42_RS01220 0.22 -0.2 277,142 + RR42_RS01220 0.22 +0.5 277,142 + RR42_RS01220 0.22 -0.5 277,142 + RR42_RS01220 0.22 +0.7 277,143 - RR42_RS01220 0.22 +0.8 277,143 - RR42_RS01220 0.22 +0.1 277,143 - RR42_RS01220 0.22 +3.9 277,143 - RR42_RS01220 0.22 +1.3 277,143 - RR42_RS01220 0.22 -0.4 277,421 + RR42_RS01220 0.58 -0.1 277,421 + RR42_RS01220 0.58 +1.7 277,421 + RR42_RS01220 0.58 +0.5 277,422 - RR42_RS01220 0.58 -2.0 277,448 + RR42_RS01220 0.62 -0.7 277,463 + RR42_RS01220 0.63 -2.0 277,548 + RR42_RS01220 0.74 -0.8 277,548 + RR42_RS01220 0.74 +0.1 277,549 - RR42_RS01220 0.75 -1.3 277,568 + RR42_RS01220 0.77 +0.2 277,577 + RR42_RS01220 0.78 +0.7 277,577 + RR42_RS01220 0.78 +0.8 277,734 + +0.1
Or see this region's nucleotide sequence