Experiment: R2A with Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01195 and RR42_RS01200 are separated by 64 nucleotides RR42_RS01200 and RR42_RS01205 are separated by 29 nucleotides RR42_RS01205 and RR42_RS01210 are separated by 145 nucleotides
RR42_RS01195: RR42_RS01195 - excinuclease ATPase, at 273,798 to 274,271
_RS01195
RR42_RS01200: RR42_RS01200 - hypothetical protein, at 274,336 to 275,145
_RS01200
RR42_RS01205: RR42_RS01205 - transposase, at 275,175 to 275,579
_RS01205
RR42_RS01210: RR42_RS01210 - n-acetyl-gamma-glutamyl-phosphate reductase, at 275,725 to 276,672
_RS01210
Position (kb) 275
276 Strain fitness (log2 ratio) -2
-1
0
1 at 274.200 kb on + strand, within RR42_RS01195 at 274.200 kb on + strand, within RR42_RS01195 at 274.201 kb on - strand, within RR42_RS01195 at 274.224 kb on + strand at 274.305 kb on - strand at 274.305 kb on - strand at 274.305 kb on - strand at 274.305 kb on - strand at 274.305 kb on - strand at 275.617 kb on + strand at 275.617 kb on + strand at 275.703 kb on - strand at 275.703 kb on - strand at 275.710 kb on - strand at 275.718 kb on - strand at 275.966 kb on + strand, within RR42_RS01210 at 275.966 kb on + strand, within RR42_RS01210 at 275.967 kb on - strand, within RR42_RS01210 at 276.230 kb on + strand, within RR42_RS01210 at 276.399 kb on - strand, within RR42_RS01210 at 276.450 kb on + strand, within RR42_RS01210 at 276.451 kb on - strand, within RR42_RS01210
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml remove 274,200 + RR42_RS01195 0.85 +1.4 274,200 + RR42_RS01195 0.85 +0.1 274,201 - RR42_RS01195 0.85 -1.0 274,224 + +0.8 274,305 - -0.6 274,305 - +0.6 274,305 - -0.3 274,305 - -0.1 274,305 - +0.1 275,617 + +0.8 275,617 + +1.7 275,703 - -0.4 275,703 - -1.6 275,710 - +0.5 275,718 - +0.5 275,966 + RR42_RS01210 0.25 -0.3 275,966 + RR42_RS01210 0.25 -1.2 275,967 - RR42_RS01210 0.26 -0.5 276,230 + RR42_RS01210 0.53 -1.3 276,399 - RR42_RS01210 0.71 -1.8 276,450 + RR42_RS01210 0.76 +0.5 276,451 - RR42_RS01210 0.77 -0.3
Or see this region's nucleotide sequence