Experiment: R2A with Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01100 and RR42_RS01105 are separated by 94 nucleotides RR42_RS01105 and RR42_RS01110 are separated by 0 nucleotides RR42_RS01110 and RR42_RS01115 overlap by 4 nucleotides RR42_RS01115 and RR42_RS01120 are separated by 90 nucleotides RR42_RS01120 and RR42_RS01125 are separated by 294 nucleotides
RR42_RS01100: RR42_RS01100 - LysR family transcriptional regulator, at 256,406 to 257,341
_RS01100
RR42_RS01105: RR42_RS01105 - membrane protein, at 257,436 to 257,948
_RS01105
RR42_RS01110: RR42_RS01110 - hypothetical protein, at 257,949 to 258,176
_RS01110
RR42_RS01115: RR42_RS01115 - hypothetical protein, at 258,173 to 258,400
_RS01115
RR42_RS01120: RR42_RS01120 - RNA polymerase sigma factor, at 258,491 to 259,072
_RS01120
RR42_RS01125: RR42_RS01125 - acetylglutamate kinase, at 259,367 to 260,284
_RS01125
Position (kb) 258
259 Strain fitness (log2 ratio) -2
-1
0
1
2 at 257.273 kb on - strand at 257.273 kb on - strand at 257.273 kb on - strand at 257.273 kb on - strand at 257.350 kb on + strand at 257.350 kb on + strand at 257.360 kb on - strand at 257.392 kb on - strand at 257.706 kb on + strand, within RR42_RS01105 at 257.707 kb on - strand, within RR42_RS01105 at 257.707 kb on - strand, within RR42_RS01105 at 257.707 kb on - strand, within RR42_RS01105 at 257.707 kb on - strand, within RR42_RS01105 at 257.712 kb on - strand, within RR42_RS01105 at 257.712 kb on - strand, within RR42_RS01105 at 257.712 kb on - strand, within RR42_RS01105 at 257.757 kb on - strand, within RR42_RS01105 at 258.391 kb on + strand at 258.392 kb on - strand at 258.552 kb on + strand, within RR42_RS01120 at 258.553 kb on - strand, within RR42_RS01120 at 258.553 kb on - strand, within RR42_RS01120 at 258.571 kb on + strand, within RR42_RS01120 at 258.571 kb on + strand, within RR42_RS01120 at 258.572 kb on - strand, within RR42_RS01120 at 258.572 kb on - strand, within RR42_RS01120 at 258.599 kb on + strand, within RR42_RS01120 at 258.620 kb on + strand, within RR42_RS01120 at 258.620 kb on + strand, within RR42_RS01120 at 258.748 kb on + strand, within RR42_RS01120 at 258.749 kb on - strand, within RR42_RS01120 at 258.753 kb on + strand, within RR42_RS01120 at 258.753 kb on + strand, within RR42_RS01120 at 258.753 kb on + strand, within RR42_RS01120 at 258.754 kb on - strand, within RR42_RS01120 at 258.872 kb on - strand, within RR42_RS01120 at 258.872 kb on - strand, within RR42_RS01120 at 258.906 kb on + strand, within RR42_RS01120 at 258.906 kb on + strand, within RR42_RS01120 at 258.906 kb on + strand, within RR42_RS01120 at 258.907 kb on - strand, within RR42_RS01120 at 258.980 kb on - strand, within RR42_RS01120 at 259.114 kb on + strand at 259.114 kb on + strand at 259.114 kb on + strand at 259.114 kb on + strand at 259.115 kb on - strand at 259.115 kb on - strand at 259.115 kb on - strand at 259.115 kb on - strand at 259.115 kb on - strand at 259.158 kb on + strand at 259.158 kb on + strand at 259.181 kb on + strand at 259.247 kb on - strand at 259.279 kb on + strand at 259.336 kb on + strand at 259.336 kb on + strand at 259.336 kb on + strand at 259.336 kb on + strand at 259.336 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.001 mg/ml remove 257,273 - +1.7 257,273 - +1.8 257,273 - -0.7 257,273 - -0.2 257,350 + +0.4 257,350 + +0.1 257,360 - +0.7 257,392 - -0.1 257,706 + RR42_RS01105 0.53 +0.6 257,707 - RR42_RS01105 0.53 +0.4 257,707 - RR42_RS01105 0.53 +1.1 257,707 - RR42_RS01105 0.53 -0.4 257,707 - RR42_RS01105 0.53 +1.7 257,712 - RR42_RS01105 0.54 +1.1 257,712 - RR42_RS01105 0.54 +0.9 257,712 - RR42_RS01105 0.54 -1.1 257,757 - RR42_RS01105 0.63 +1.5 258,391 + -1.0 258,392 - +0.7 258,552 + RR42_RS01120 0.10 +0.5 258,553 - RR42_RS01120 0.11 +0.6 258,553 - RR42_RS01120 0.11 +0.9 258,571 + RR42_RS01120 0.14 -0.1 258,571 + RR42_RS01120 0.14 +0.1 258,572 - RR42_RS01120 0.14 -0.7 258,572 - RR42_RS01120 0.14 -1.8 258,599 + RR42_RS01120 0.19 +0.4 258,620 + RR42_RS01120 0.22 +0.1 258,620 + RR42_RS01120 0.22 +0.3 258,748 + RR42_RS01120 0.44 +0.4 258,749 - RR42_RS01120 0.44 -1.6 258,753 + RR42_RS01120 0.45 -0.6 258,753 + RR42_RS01120 0.45 +0.3 258,753 + RR42_RS01120 0.45 -0.5 258,754 - RR42_RS01120 0.45 +1.0 258,872 - RR42_RS01120 0.65 +0.6 258,872 - RR42_RS01120 0.65 -0.9 258,906 + RR42_RS01120 0.71 -0.3 258,906 + RR42_RS01120 0.71 -1.1 258,906 + RR42_RS01120 0.71 -1.0 258,907 - RR42_RS01120 0.71 +0.2 258,980 - RR42_RS01120 0.84 +0.1 259,114 + +1.1 259,114 + -0.1 259,114 + -0.0 259,114 + -1.1 259,115 - -0.1 259,115 - -0.2 259,115 - -0.0 259,115 - -1.3 259,115 - -0.2 259,158 + -2.0 259,158 + +0.8 259,181 + -0.4 259,247 - -0.5 259,279 + -0.7 259,336 + -0.1 259,336 + -0.4 259,336 + +0.7 259,336 + -1.9 259,336 + -0.4
Or see this region's nucleotide sequence