Experiment: KB with Dimethyl Sulfoxide 1 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4474 and Psyr_4475 overlap by 4 nucleotides Psyr_4475 and Psyr_4476 overlap by 8 nucleotides Psyr_4476 and Psyr_4477 are separated by 153 nucleotides
Psyr_4474: Psyr_4474 - hypothetical protein, at 5,333,982 to 5,335,508
_4474
Psyr_4475: Psyr_4475 - NAD-dependent epimerase/dehydratase, at 5,335,505 to 5,336,434
_4475
Psyr_4476: Psyr_4476 - dTDP-4-dehydrorhamnose reductase, at 5,336,427 to 5,337,311
_4476
Psyr_4477: Psyr_4477 - MscS Mechanosensitive ion channel, at 5,337,465 to 5,339,783
_4477
Position (kb) 5336
5337
5338 Strain fitness (log2 ratio) -2
-1
0
1 at 5335.499 kb on + strand at 5335.499 kb on + strand at 5335.499 kb on + strand at 5336.681 kb on - strand, within Psyr_4476 at 5336.681 kb on - strand, within Psyr_4476 at 5336.681 kb on - strand, within Psyr_4476 at 5336.878 kb on - strand, within Psyr_4476 at 5336.878 kb on - strand, within Psyr_4476 at 5337.128 kb on - strand, within Psyr_4476 at 5337.128 kb on - strand, within Psyr_4476 at 5337.128 kb on - strand, within Psyr_4476 at 5337.131 kb on - strand, within Psyr_4476 at 5337.131 kb on - strand, within Psyr_4476 at 5337.131 kb on - strand, within Psyr_4476 at 5337.131 kb on - strand, within Psyr_4476 at 5337.131 kb on - strand, within Psyr_4476 at 5337.131 kb on - strand, within Psyr_4476 at 5337.131 kb on - strand, within Psyr_4476 at 5337.131 kb on - strand, within Psyr_4476 at 5337.466 kb on + strand at 5337.467 kb on - strand at 5337.522 kb on + strand at 5337.522 kb on + strand at 5337.523 kb on - strand at 5337.523 kb on - strand at 5337.632 kb on - strand at 5337.632 kb on - strand at 5337.709 kb on + strand, within Psyr_4477 at 5337.710 kb on - strand, within Psyr_4477 at 5337.728 kb on + strand, within Psyr_4477 at 5337.729 kb on - strand, within Psyr_4477 at 5337.729 kb on - strand, within Psyr_4477 at 5337.886 kb on + strand, within Psyr_4477 at 5337.886 kb on + strand, within Psyr_4477 at 5337.886 kb on + strand, within Psyr_4477 at 5337.887 kb on - strand, within Psyr_4477 at 5337.887 kb on - strand, within Psyr_4477 at 5337.887 kb on - strand, within Psyr_4477
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Dimethyl Sulfoxide 1 vol% remove 5,335,499 + +0.5 5,335,499 + -1.3 5,335,499 + +0.3 5,336,681 - Psyr_4476 0.29 -0.7 5,336,681 - Psyr_4476 0.29 -1.2 5,336,681 - Psyr_4476 0.29 -1.0 5,336,878 - Psyr_4476 0.51 -0.7 5,336,878 - Psyr_4476 0.51 -0.7 5,337,128 - Psyr_4476 0.79 +0.1 5,337,128 - Psyr_4476 0.79 +0.3 5,337,128 - Psyr_4476 0.79 -0.3 5,337,131 - Psyr_4476 0.80 -1.2 5,337,131 - Psyr_4476 0.80 -0.2 5,337,131 - Psyr_4476 0.80 +0.3 5,337,131 - Psyr_4476 0.80 -2.0 5,337,131 - Psyr_4476 0.80 -1.2 5,337,131 - Psyr_4476 0.80 -2.3 5,337,131 - Psyr_4476 0.80 -1.2 5,337,131 - Psyr_4476 0.80 -1.5 5,337,466 + +0.5 5,337,467 - +1.7 5,337,522 + +0.5 5,337,522 + -0.7 5,337,523 - -0.1 5,337,523 - +0.3 5,337,632 - +1.3 5,337,632 - +0.2 5,337,709 + Psyr_4477 0.11 -1.0 5,337,710 - Psyr_4477 0.11 +0.8 5,337,728 + Psyr_4477 0.11 -0.8 5,337,729 - Psyr_4477 0.11 -0.1 5,337,729 - Psyr_4477 0.11 -0.0 5,337,886 + Psyr_4477 0.18 -0.2 5,337,886 + Psyr_4477 0.18 -0.5 5,337,886 + Psyr_4477 0.18 -0.2 5,337,887 - Psyr_4477 0.18 -0.6 5,337,887 - Psyr_4477 0.18 -0.6 5,337,887 - Psyr_4477 0.18 +0.7
Or see this region's nucleotide sequence