Experiment: KB with Dimethyl Sulfoxide 1 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2198 and Psyr_2199 are separated by 33 nucleotides Psyr_2199 and Psyr_2200 overlap by 4 nucleotides Psyr_2200 and Psyr_2201 are separated by 22 nucleotides Psyr_2201 and Psyr_2202 are separated by 2 nucleotides
Psyr_2198: Psyr_2198 - Urease, beta subunit:Urease, gamma subunit, at 2,556,211 to 2,556,906
_2198
Psyr_2199: Psyr_2199 - Urease accessory protein UreF, at 2,556,940 to 2,557,671
_2199
Psyr_2200: Psyr_2200 - UreE urease accessory, N-terminal, at 2,557,668 to 2,558,108
_2200
Psyr_2201: Psyr_2201 - amino acid/amide ABC transporter ATP-binding protein 2, HAAT family, at 2,558,131 to 2,558,820
_2201
Psyr_2202: Psyr_2202 - ABC transporter, at 2,558,823 to 2,559,560
_2202
Position (kb)
2557
2558
2559 Strain fitness (log2 ratio)
-2
-1
0
1 at 2556.866 kb on + strand at 2556.929 kb on + strand at 2556.930 kb on - strand at 2557.801 kb on + strand, within Psyr_2200 at 2557.849 kb on + strand, within Psyr_2200 at 2557.850 kb on - strand, within Psyr_2200 at 2558.474 kb on + strand, within Psyr_2201 at 2558.486 kb on + strand, within Psyr_2201 at 2558.486 kb on + strand, within Psyr_2201 at 2558.487 kb on - strand, within Psyr_2201 at 2558.609 kb on + strand, within Psyr_2201 at 2558.792 kb on + strand at 2558.792 kb on + strand at 2558.792 kb on + strand at 2558.792 kb on + strand at 2558.793 kb on - strand at 2558.885 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Dimethyl Sulfoxide 1 vol% remove 2,556,866 + -2.0 2,556,929 + -2.1 2,556,930 - -0.4 2,557,801 + Psyr_2200 0.30 -0.1 2,557,849 + Psyr_2200 0.41 -1.6 2,557,850 - Psyr_2200 0.41 -0.4 2,558,474 + Psyr_2201 0.50 +0.5 2,558,486 + Psyr_2201 0.51 +0.1 2,558,486 + Psyr_2201 0.51 +0.1 2,558,487 - Psyr_2201 0.52 +0.2 2,558,609 + Psyr_2201 0.69 -0.4 2,558,792 + -1.2 2,558,792 + +1.0 2,558,792 + -1.0 2,558,792 + -0.0 2,558,793 - -0.5 2,558,885 - +1.3
Or see this region's nucleotide sequence