Experiment: KB with Dimethyl Sulfoxide 1 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1921 and Psyr_1922 are separated by 15 nucleotides Psyr_1922 and Psyr_1923 are separated by 43 nucleotides Psyr_1923 and Psyr_1924 overlap by 26 nucleotides Psyr_1924 and Psyr_1925 are separated by 29 nucleotides
Psyr_1921: Psyr_1921 - Alpha/beta hydrolase fold protein, at 2,194,112 to 2,195,221
_1921
Psyr_1922: Psyr_1922 - UBA/THIF-type NAD/FAD binding fold protein, at 2,195,237 to 2,196,166
_1922
Psyr_1923: Psyr_1923 - hypothetical protein, at 2,196,210 to 2,197,433
_1923
Psyr_1924: Psyr_1924 - hypothetical protein, at 2,197,408 to 2,198,067
_1924
Psyr_1925: Psyr_1925 - diaminobutyrate aminotransferase apoenzyme, at 2,198,097 to 2,199,377
_1925
Position (kb)
2196
2197
2198 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2195.219 kb on + strand at 2195.219 kb on + strand at 2195.220 kb on - strand at 2195.292 kb on - strand at 2195.292 kb on - strand at 2195.292 kb on - strand at 2195.445 kb on + strand, within Psyr_1922 at 2195.445 kb on + strand, within Psyr_1922 at 2195.446 kb on - strand, within Psyr_1922 at 2195.480 kb on + strand, within Psyr_1922 at 2195.533 kb on + strand, within Psyr_1922 at 2195.609 kb on - strand, within Psyr_1922 at 2195.610 kb on + strand, within Psyr_1922 at 2195.610 kb on + strand, within Psyr_1922 at 2195.610 kb on + strand, within Psyr_1922 at 2195.610 kb on + strand, within Psyr_1922 at 2195.611 kb on - strand, within Psyr_1922 at 2195.611 kb on - strand, within Psyr_1922 at 2195.611 kb on - strand, within Psyr_1922 at 2195.611 kb on - strand, within Psyr_1922 at 2195.675 kb on + strand, within Psyr_1922 at 2195.675 kb on + strand, within Psyr_1922 at 2195.676 kb on - strand, within Psyr_1922 at 2195.676 kb on - strand, within Psyr_1922 at 2195.676 kb on - strand, within Psyr_1922 at 2195.693 kb on + strand, within Psyr_1922 at 2195.694 kb on - strand, within Psyr_1922 at 2195.717 kb on - strand, within Psyr_1922 at 2195.777 kb on + strand, within Psyr_1922 at 2195.777 kb on + strand, within Psyr_1922 at 2195.777 kb on + strand, within Psyr_1922 at 2195.777 kb on + strand, within Psyr_1922 at 2195.777 kb on + strand, within Psyr_1922 at 2195.777 kb on + strand, within Psyr_1922 at 2195.777 kb on + strand, within Psyr_1922 at 2195.777 kb on + strand, within Psyr_1922 at 2195.778 kb on - strand, within Psyr_1922 at 2195.778 kb on - strand, within Psyr_1922 at 2195.778 kb on - strand, within Psyr_1922 at 2195.885 kb on + strand, within Psyr_1922 at 2195.885 kb on + strand, within Psyr_1922 at 2195.885 kb on + strand, within Psyr_1922 at 2195.886 kb on - strand, within Psyr_1922 at 2195.950 kb on + strand, within Psyr_1922 at 2195.950 kb on + strand, within Psyr_1922 at 2195.951 kb on - strand, within Psyr_1922 at 2196.036 kb on - strand, within Psyr_1922 at 2196.065 kb on + strand, within Psyr_1922 at 2196.065 kb on + strand, within Psyr_1922 at 2196.065 kb on + strand, within Psyr_1922 at 2196.065 kb on + strand, within Psyr_1922 at 2196.065 kb on + strand, within Psyr_1922 at 2196.066 kb on - strand, within Psyr_1922 at 2196.066 kb on - strand, within Psyr_1922 at 2196.066 kb on - strand, within Psyr_1922 at 2196.066 kb on - strand, within Psyr_1922 at 2196.066 kb on - strand, within Psyr_1922 at 2196.066 kb on - strand, within Psyr_1922 at 2196.066 kb on - strand, within Psyr_1922 at 2196.066 kb on - strand, within Psyr_1922 at 2196.066 kb on - strand, within Psyr_1922 at 2196.066 kb on - strand, within Psyr_1922 at 2196.066 kb on - strand at 2196.066 kb on - strand, within Psyr_1922 at 2196.068 kb on + strand, within Psyr_1922 at 2196.068 kb on + strand, within Psyr_1922 at 2196.068 kb on + strand, within Psyr_1922 at 2196.069 kb on - strand, within Psyr_1922 at 2196.085 kb on + strand at 2196.085 kb on + strand at 2196.085 kb on + strand at 2196.086 kb on - strand at 2196.086 kb on - strand at 2196.100 kb on + strand at 2196.100 kb on + strand at 2196.100 kb on + strand at 2196.101 kb on - strand at 2196.101 kb on - strand at 2196.101 kb on - strand at 2196.104 kb on + strand at 2196.104 kb on + strand at 2196.104 kb on + strand at 2196.245 kb on + strand at 2196.245 kb on + strand at 2196.245 kb on + strand at 2196.245 kb on + strand at 2196.246 kb on - strand at 2196.246 kb on - strand at 2196.246 kb on - strand at 2196.246 kb on - strand at 2196.246 kb on - strand at 2196.295 kb on + strand at 2196.296 kb on - strand at 2196.304 kb on + strand at 2196.304 kb on + strand at 2196.304 kb on + strand at 2196.405 kb on + strand, within Psyr_1923 at 2196.405 kb on + strand, within Psyr_1923 at 2196.405 kb on + strand, within Psyr_1923 at 2196.405 kb on + strand, within Psyr_1923 at 2196.406 kb on - strand, within Psyr_1923 at 2196.406 kb on - strand, within Psyr_1923 at 2196.459 kb on + strand, within Psyr_1923 at 2196.505 kb on - strand, within Psyr_1923 at 2196.505 kb on - strand, within Psyr_1923 at 2196.522 kb on + strand, within Psyr_1923 at 2196.522 kb on + strand, within Psyr_1923 at 2196.523 kb on - strand, within Psyr_1923 at 2196.540 kb on + strand, within Psyr_1923 at 2196.540 kb on + strand, within Psyr_1923 at 2196.540 kb on + strand, within Psyr_1923 at 2196.540 kb on + strand, within Psyr_1923 at 2196.595 kb on - strand, within Psyr_1923 at 2196.595 kb on - strand, within Psyr_1923 at 2196.604 kb on + strand, within Psyr_1923 at 2196.604 kb on + strand, within Psyr_1923 at 2196.605 kb on - strand, within Psyr_1923 at 2196.605 kb on - strand, within Psyr_1923 at 2196.605 kb on - strand, within Psyr_1923 at 2196.605 kb on - strand, within Psyr_1923 at 2196.605 kb on - strand, within Psyr_1923 at 2196.638 kb on + strand, within Psyr_1923 at 2196.638 kb on + strand, within Psyr_1923 at 2196.640 kb on + strand, within Psyr_1923 at 2196.640 kb on + strand at 2196.641 kb on - strand, within Psyr_1923 at 2196.641 kb on - strand, within Psyr_1923 at 2196.771 kb on + strand, within Psyr_1923 at 2196.771 kb on + strand, within Psyr_1923 at 2196.771 kb on + strand, within Psyr_1923 at 2196.771 kb on + strand, within Psyr_1923 at 2196.771 kb on + strand, within Psyr_1923 at 2196.771 kb on + strand, within Psyr_1923 at 2196.772 kb on - strand, within Psyr_1923 at 2196.772 kb on - strand, within Psyr_1923 at 2196.772 kb on - strand, within Psyr_1923 at 2196.773 kb on + strand, within Psyr_1923 at 2196.774 kb on - strand, within Psyr_1923 at 2196.774 kb on - strand, within Psyr_1923 at 2196.774 kb on - strand, within Psyr_1923 at 2196.774 kb on - strand, within Psyr_1923 at 2196.774 kb on - strand, within Psyr_1923 at 2196.774 kb on - strand, within Psyr_1923 at 2196.871 kb on + strand, within Psyr_1923 at 2196.934 kb on + strand, within Psyr_1923 at 2196.935 kb on - strand, within Psyr_1923 at 2196.938 kb on + strand, within Psyr_1923 at 2196.938 kb on + strand, within Psyr_1923 at 2196.938 kb on + strand, within Psyr_1923 at 2196.938 kb on + strand, within Psyr_1923 at 2196.938 kb on + strand, within Psyr_1923 at 2196.938 kb on + strand, within Psyr_1923 at 2196.939 kb on - strand, within Psyr_1923 at 2196.939 kb on - strand, within Psyr_1923 at 2196.939 kb on - strand, within Psyr_1923 at 2196.939 kb on - strand, within Psyr_1923 at 2196.939 kb on - strand, within Psyr_1923 at 2196.939 kb on - strand, within Psyr_1923 at 2197.052 kb on + strand, within Psyr_1923 at 2197.053 kb on - strand, within Psyr_1923 at 2197.057 kb on + strand, within Psyr_1923 at 2197.094 kb on - strand, within Psyr_1923 at 2197.185 kb on - strand, within Psyr_1923 at 2197.226 kb on + strand, within Psyr_1923 at 2197.279 kb on + strand, within Psyr_1923 at 2197.331 kb on - strand at 2197.381 kb on - strand at 2197.383 kb on - strand at 2197.386 kb on + strand at 2197.386 kb on + strand at 2197.386 kb on + strand at 2197.386 kb on + strand at 2197.386 kb on + strand at 2197.386 kb on + strand at 2197.387 kb on - strand at 2197.387 kb on - strand at 2197.461 kb on + strand at 2197.461 kb on + strand at 2197.469 kb on + strand at 2197.469 kb on + strand at 2197.470 kb on - strand at 2197.526 kb on - strand, within Psyr_1924 at 2197.526 kb on - strand, within Psyr_1924 at 2197.526 kb on - strand, within Psyr_1924 at 2197.526 kb on - strand, within Psyr_1924 at 2197.526 kb on - strand, within Psyr_1924 at 2197.539 kb on - strand, within Psyr_1924 at 2197.600 kb on + strand, within Psyr_1924 at 2197.800 kb on + strand, within Psyr_1924 at 2197.855 kb on + strand, within Psyr_1924 at 2197.884 kb on + strand, within Psyr_1924 at 2197.885 kb on - strand, within Psyr_1924 at 2197.902 kb on - strand, within Psyr_1924 at 2197.977 kb on + strand, within Psyr_1924 at 2197.978 kb on - strand, within Psyr_1924 at 2197.978 kb on - strand, within Psyr_1924 at 2198.047 kb on + strand at 2198.047 kb on + strand at 2198.047 kb on + strand at 2198.047 kb on + strand at 2198.048 kb on - strand at 2198.048 kb on - strand at 2198.048 kb on - strand at 2198.048 kb on - strand at 2198.066 kb on - strand at 2198.070 kb on + strand at 2198.118 kb on + strand at 2198.118 kb on + strand at 2198.119 kb on - strand at 2198.119 kb on - strand at 2198.253 kb on + strand, within Psyr_1925 at 2198.253 kb on + strand, within Psyr_1925 at 2198.253 kb on + strand, within Psyr_1925 at 2198.295 kb on + strand, within Psyr_1925 at 2198.296 kb on - strand, within Psyr_1925 at 2198.296 kb on - strand, within Psyr_1925 at 2198.296 kb on - strand, within Psyr_1925 at 2198.297 kb on + strand, within Psyr_1925 at 2198.297 kb on + strand, within Psyr_1925 at 2198.298 kb on - strand, within Psyr_1925 at 2198.378 kb on + strand, within Psyr_1925 at 2198.404 kb on - strand, within Psyr_1925 at 2198.404 kb on - strand, within Psyr_1925 at 2198.404 kb on - strand, within Psyr_1925 at 2198.404 kb on - strand, within Psyr_1925
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Dimethyl Sulfoxide 1 vol% remove 2,195,219 + +0.6 2,195,219 + -1.3 2,195,220 - +0.0 2,195,292 - +0.3 2,195,292 - -0.4 2,195,292 - +1.2 2,195,445 + Psyr_1922 0.22 +0.2 2,195,445 + Psyr_1922 0.22 +1.6 2,195,446 - Psyr_1922 0.22 -1.7 2,195,480 + Psyr_1922 0.26 -1.5 2,195,533 + Psyr_1922 0.32 -0.3 2,195,609 - Psyr_1922 0.40 -1.3 2,195,610 + Psyr_1922 0.40 +0.0 2,195,610 + Psyr_1922 0.40 -0.1 2,195,610 + Psyr_1922 0.40 +0.9 2,195,610 + Psyr_1922 0.40 +1.9 2,195,611 - Psyr_1922 0.40 -2.3 2,195,611 - Psyr_1922 0.40 -2.1 2,195,611 - Psyr_1922 0.40 +0.1 2,195,611 - Psyr_1922 0.40 -2.0 2,195,675 + Psyr_1922 0.47 -0.4 2,195,675 + Psyr_1922 0.47 -2.6 2,195,676 - Psyr_1922 0.47 -0.9 2,195,676 - Psyr_1922 0.47 -2.2 2,195,676 - Psyr_1922 0.47 -2.6 2,195,693 + Psyr_1922 0.49 +0.0 2,195,694 - Psyr_1922 0.49 -0.5 2,195,717 - Psyr_1922 0.52 -2.6 2,195,777 + Psyr_1922 0.58 -0.8 2,195,777 + Psyr_1922 0.58 +0.1 2,195,777 + Psyr_1922 0.58 -0.4 2,195,777 + Psyr_1922 0.58 +0.9 2,195,777 + Psyr_1922 0.58 -1.4 2,195,777 + Psyr_1922 0.58 -0.8 2,195,777 + Psyr_1922 0.58 +0.0 2,195,777 + Psyr_1922 0.58 +0.7 2,195,778 - Psyr_1922 0.58 -0.3 2,195,778 - Psyr_1922 0.58 +0.4 2,195,778 - Psyr_1922 0.58 -0.0 2,195,885 + Psyr_1922 0.70 +0.1 2,195,885 + Psyr_1922 0.70 -0.0 2,195,885 + Psyr_1922 0.70 -0.5 2,195,886 - Psyr_1922 0.70 -0.7 2,195,950 + Psyr_1922 0.77 -0.0 2,195,950 + Psyr_1922 0.77 +0.3 2,195,951 - Psyr_1922 0.77 +0.6 2,196,036 - Psyr_1922 0.86 -0.9 2,196,065 + Psyr_1922 0.89 +0.4 2,196,065 + Psyr_1922 0.89 +0.3 2,196,065 + Psyr_1922 0.89 -0.1 2,196,065 + Psyr_1922 0.89 -0.4 2,196,065 + Psyr_1922 0.89 -1.4 2,196,066 - Psyr_1922 0.89 -0.3 2,196,066 - Psyr_1922 0.89 +0.3 2,196,066 - Psyr_1922 0.89 -0.3 2,196,066 - Psyr_1922 0.89 +0.1 2,196,066 - Psyr_1922 0.89 -0.4 2,196,066 - Psyr_1922 0.89 -0.2 2,196,066 - Psyr_1922 0.89 -0.9 2,196,066 - Psyr_1922 0.89 -1.5 2,196,066 - Psyr_1922 0.89 -2.2 2,196,066 - Psyr_1922 0.89 +0.4 2,196,066 - +0.6 2,196,066 - Psyr_1922 0.89 -1.3 2,196,068 + Psyr_1922 0.89 +2.3 2,196,068 + Psyr_1922 0.89 -0.7 2,196,068 + Psyr_1922 0.89 -0.1 2,196,069 - Psyr_1922 0.89 +0.9 2,196,085 + +0.2 2,196,085 + +0.3 2,196,085 + +1.0 2,196,086 - -0.3 2,196,086 - +0.2 2,196,100 + -0.6 2,196,100 + +0.0 2,196,100 + -0.7 2,196,101 - +0.6 2,196,101 - -0.5 2,196,101 - +1.3 2,196,104 + -0.7 2,196,104 + +0.0 2,196,104 + +1.3 2,196,245 + +0.8 2,196,245 + -0.9 2,196,245 + -0.6 2,196,245 + +0.1 2,196,246 - -0.3 2,196,246 - +0.6 2,196,246 - +0.1 2,196,246 - +1.2 2,196,246 - +0.5 2,196,295 + +1.3 2,196,296 - -0.5 2,196,304 + +0.9 2,196,304 + +2.9 2,196,304 + +0.9 2,196,405 + Psyr_1923 0.16 -0.3 2,196,405 + Psyr_1923 0.16 +0.6 2,196,405 + Psyr_1923 0.16 -0.4 2,196,405 + Psyr_1923 0.16 +0.9 2,196,406 - Psyr_1923 0.16 -0.1 2,196,406 - Psyr_1923 0.16 +1.2 2,196,459 + Psyr_1923 0.20 +0.4 2,196,505 - Psyr_1923 0.24 -0.1 2,196,505 - Psyr_1923 0.24 +1.5 2,196,522 + Psyr_1923 0.25 +1.9 2,196,522 + Psyr_1923 0.25 +0.1 2,196,523 - Psyr_1923 0.26 -1.6 2,196,540 + Psyr_1923 0.27 -0.1 2,196,540 + Psyr_1923 0.27 -2.6 2,196,540 + Psyr_1923 0.27 -1.0 2,196,540 + Psyr_1923 0.27 -1.1 2,196,595 - Psyr_1923 0.31 -0.9 2,196,595 - Psyr_1923 0.31 +0.9 2,196,604 + Psyr_1923 0.32 +0.9 2,196,604 + Psyr_1923 0.32 -1.7 2,196,605 - Psyr_1923 0.32 -0.1 2,196,605 - Psyr_1923 0.32 +0.3 2,196,605 - Psyr_1923 0.32 +0.0 2,196,605 - Psyr_1923 0.32 -0.5 2,196,605 - Psyr_1923 0.32 +0.6 2,196,638 + Psyr_1923 0.35 +0.2 2,196,638 + Psyr_1923 0.35 +0.6 2,196,640 + Psyr_1923 0.35 +0.2 2,196,640 + +0.3 2,196,641 - Psyr_1923 0.35 -0.7 2,196,641 - Psyr_1923 0.35 +1.2 2,196,771 + Psyr_1923 0.46 -0.3 2,196,771 + Psyr_1923 0.46 -0.7 2,196,771 + Psyr_1923 0.46 -0.1 2,196,771 + Psyr_1923 0.46 -0.4 2,196,771 + Psyr_1923 0.46 -0.8 2,196,771 + Psyr_1923 0.46 -0.2 2,196,772 - Psyr_1923 0.46 -1.1 2,196,772 - Psyr_1923 0.46 -1.0 2,196,772 - Psyr_1923 0.46 -1.2 2,196,773 + Psyr_1923 0.46 +1.0 2,196,774 - Psyr_1923 0.46 -2.7 2,196,774 - Psyr_1923 0.46 -1.1 2,196,774 - Psyr_1923 0.46 -0.1 2,196,774 - Psyr_1923 0.46 -2.2 2,196,774 - Psyr_1923 0.46 -1.2 2,196,774 - Psyr_1923 0.46 +0.6 2,196,871 + Psyr_1923 0.54 -1.0 2,196,934 + Psyr_1923 0.59 -2.2 2,196,935 - Psyr_1923 0.59 -0.8 2,196,938 + Psyr_1923 0.59 +0.0 2,196,938 + Psyr_1923 0.59 -1.4 2,196,938 + Psyr_1923 0.59 -0.7 2,196,938 + Psyr_1923 0.59 +0.2 2,196,938 + Psyr_1923 0.59 -1.7 2,196,938 + Psyr_1923 0.59 +0.2 2,196,939 - Psyr_1923 0.60 -1.3 2,196,939 - Psyr_1923 0.60 +0.7 2,196,939 - Psyr_1923 0.60 +0.6 2,196,939 - Psyr_1923 0.60 +0.9 2,196,939 - Psyr_1923 0.60 -1.4 2,196,939 - Psyr_1923 0.60 +0.6 2,197,052 + Psyr_1923 0.69 +1.6 2,197,053 - Psyr_1923 0.69 +1.1 2,197,057 + Psyr_1923 0.69 -0.4 2,197,094 - Psyr_1923 0.72 +0.2 2,197,185 - Psyr_1923 0.80 -0.3 2,197,226 + Psyr_1923 0.83 -0.4 2,197,279 + Psyr_1923 0.87 +0.4 2,197,331 - -2.9 2,197,381 - -1.9 2,197,383 - +2.9 2,197,386 + +0.9 2,197,386 + +0.9 2,197,386 + +1.3 2,197,386 + -0.4 2,197,386 + +0.6 2,197,386 + +0.9 2,197,387 - -1.4 2,197,387 - -2.4 2,197,461 + -1.3 2,197,461 + -0.1 2,197,469 + +0.6 2,197,469 + -1.2 2,197,470 - +0.2 2,197,526 - Psyr_1924 0.18 -1.0 2,197,526 - Psyr_1924 0.18 -0.9 2,197,526 - Psyr_1924 0.18 -2.6 2,197,526 - Psyr_1924 0.18 -3.4 2,197,526 - Psyr_1924 0.18 -1.9 2,197,539 - Psyr_1924 0.20 -1.1 2,197,600 + Psyr_1924 0.29 -0.4 2,197,800 + Psyr_1924 0.59 -0.4 2,197,855 + Psyr_1924 0.68 -0.8 2,197,884 + Psyr_1924 0.72 +1.2 2,197,885 - Psyr_1924 0.72 -0.9 2,197,902 - Psyr_1924 0.75 +1.2 2,197,977 + Psyr_1924 0.86 +1.2 2,197,978 - Psyr_1924 0.86 -0.1 2,197,978 - Psyr_1924 0.86 -0.6 2,198,047 + +1.6 2,198,047 + +0.6 2,198,047 + +1.8 2,198,047 + -0.8 2,198,048 - +1.0 2,198,048 - -0.4 2,198,048 - -0.1 2,198,048 - -0.4 2,198,066 - +1.6 2,198,070 + -0.1 2,198,118 + -0.6 2,198,118 + +0.5 2,198,119 - -1.3 2,198,119 - -1.1 2,198,253 + Psyr_1925 0.12 +0.0 2,198,253 + Psyr_1925 0.12 -0.0 2,198,253 + Psyr_1925 0.12 +0.1 2,198,295 + Psyr_1925 0.15 -2.3 2,198,296 - Psyr_1925 0.16 -1.4 2,198,296 - Psyr_1925 0.16 -2.7 2,198,296 - Psyr_1925 0.16 +0.6 2,198,297 + Psyr_1925 0.16 -1.0 2,198,297 + Psyr_1925 0.16 -0.4 2,198,298 - Psyr_1925 0.16 -2.4 2,198,378 + Psyr_1925 0.22 -2.7 2,198,404 - Psyr_1925 0.24 +2.4 2,198,404 - Psyr_1925 0.24 -0.3 2,198,404 - Psyr_1925 0.24 -1.6 2,198,404 - Psyr_1925 0.24 -1.0
Or see this region's nucleotide sequence